Development of a Wake Forest Multi-Species NHP Biorepository to Support Interdisciplinary Aging Studies

开发维克森林多物种 NHP 生物样本库以支持跨学科衰老研究

• 批准号: 10468876
• 负责人: Laura A Cox
• 金额: $ 17.82万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10468876
• 关键词: Adult; Age; Aging; Alzheimer' s Disease; Animals; Biological; Biological Clocks; Blood; Blood specimen; Cardiovascular Diseases; Cardiovascular Models; Chronology; Clinical; Clinical Markers; Clinical Research; Computer software; Controlled Environment; DNA Methylation; Data; Data Management Resources; Data Storage and Retrieval; Databases; Dementia; Development; Diabetes Mellitus; Diet; Dietary Intervention; Disease; Equipment and supply inventories; Female; Funding; Future; Gene Expression; Genetic; Genetic Predisposition to Disease; Genotype; Goals; Health; Hormones; Human; Interdisciplinary Study; Intervention; Lead; Life Cycle Stages; Life Style; Liver; Longevity; Macaca fascicularis; Macaca mulatta; Measures; Metabolic; Metabolic Diseases; Modeling; Molecular; Molecular Profiling; Monkeys; Noise; Obesity; Paper; Papio; Pathway interactions; Phase; Phenotype; Physiological; Plasma; Primates; Process; Proteins; RNA; Radiation exposure; Records; Research; Research Personnel; Resources; Rhesus; Sampling; Stress; Systems Integration; Tissues; Translating; United States National Institutes of Health; Work; age related; base; biobank; clinical development; clinically relevant; cohort; comparative; data harmonization; data management; data sharing; database query; forest; genetic pedigree; human disease; immune function; male; nonhuman primate; normal aging; open source; phenotypic data; protein metabolite; sample archive; stressor; vervet

ASTRACT Non-human primates (NHP) such as baboon, cynomolgus macaque, rhesus macaque, and vervet are susceptible to the same age-related health challenges and diseases as humans. The overall aging trajectory in NHP is also influenced by stressors and lifestyle similar to humans. Research in NHP allows for controlled environments, providing longitudinal data with less “noise” than in human studies. The genetic, metabolic and physiologic similarities between NHP and humans make findings in NHP directly translatable to our understanding of human disease processes, including genetic predispositions and early molecular indicators of these processes. In the R21 phase of this project, we will 1) harmonize existing samples and data in four NHP species that can be leveraged to better understand molecular and cellular mechanisms underlying human aging, and 2) implement the Monkey Inventory and Data management of Samples (MIDAS), a LabKey Server data management system, for integration of omic data and pedigree data with clinical and research data from Aim 1. In the subsequent R33 phase, we will demonstrate the unique value of this harmonized, comparative cross- species resource for aging studies using integrated omics and clinical measures to quantify aging in liver and plasma across the lifespan in these four NHP. We will 1) determine the biological age trajectory for each NHP species from multiple chronological ages that capture human equivalent 18-80 years, by measuring molecules known to reflect biological age including gene expression, DNA methylation, and proteins in liver samples (n=48 for each species (F, 24; M,24)); and 2) identify circulating molecular signatures that correlate with liver specific signatures of aging that precede clinical markers of aging common to the four NHP species Alignment and harmonization of data and samples for these four NHP cohorts will provide a critical resource translating discoveries to humans, and supporting interdisciplinary studies of aging across the lifespan.

ASTRACT