Defining the descending pain modulatory circuit

定义下行疼痛调节回路

• 批准号: 10461117
• 负责人: Mary Magdalen Heinricher
• 金额: $ 4.77万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2022-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10461117
• 关键词: Absence of pain sensation; Acute Pain; Adult; Area; Automobile Driving; Behavior; Behavioral; Brain; Brain Stem; Cells; Complement; Complex; Development; Electrophysiology (science); Equilibrium; Freund' s Adjuvant; Goals; Heterogeneity; Hyperalgesia; In Vitro; Inflammation; Inflammatory; Injections; Label; Laboratories; Link; Maps; Maternal Behavior; Measures; Mediating; Molecular; Neuraxis; Neurons; Nociception; Opioid; Opioid Analgesics; Output; Pain; Pain management; Pathway interactions; Peripheral; Persistent pain; Pharmacology; Physiological; Population; Property; Rattus; Reproduction; Role; Sensory; Slice; Spinal cord posterior horn; Stress; Synapses; Synaptic plasticity; System; Testing; Viral; Work; cell type; chronic pain; dorsal horn; experimental study; in vivo; insight; midbrain central gray substance; mu opioid receptors; neurotransmitter release; optogenetics; pain behavior; pain inhibition; recruit; response; transmission process

PROJECT SUMMARY The central nervous system has an intrinsic pain modulatory system that regulates nociceptive processing through descending projections from the brainstem to the spinal cord dorsal horn. The ventrolateral periaqueductal gray (vlPAG) integrates sensory information with input from higher cortical and subcortical areas, and sends projections to the rostral ventromedial medulla (RVM) that are relayed to the dorsal horn of the spinal cord. Both the vlPAG and RVM are heterogenous with respect to participating in multiple behavioral circuits. The proposed studies build on extensive previous work from the Heinricher laboratory that has defined the output from the RVM, showing that bidirectional pain control from this region is mediated by two physiologically defined cell classes, “ON-cells” and “OFF-cells,” that respectively facilitate and inhibit dorsal horn nociceptive transmission under different conditions. The Ingram laboratory has expertise studying opioid actions within the PAG and RVM, as well as adaptations in both areas with persistent inflammation. Proposed viral optogenetic strategies will map and define the vlPAG circuit that regulates RVM ON-cells involved in the facilitation of pain and elucidate underlying cellular mechanisms that shift the balance of RVM output from inhibition of pain to facilitation of pain with persistent inflammation. The combined expertise of the two laboratories will focus on identified PAG-RVM synapses using optogenetic stimulation of RVM terminals originating from the PAG. In vitro brain-slice recordings (Ingram lab) will examine the heterogeneity of PAG output to the RVM and PAG-RVM synapses, as well as cellular mechanisms of synaptic plasticity induced in persistent inflammation. These studies will use a fluorescent label for the μ-opioid receptor to differentiate presumed ON-cells from other classes in the slice to determine whether PAG terminals directly synapse on ON-cells, OFF-cells, or both, as well as what neurotransmitters are released. In vivo single-cell recording studies (Heinricher lab) will determine how inflammation-induced changes in PAG-RVM synapses control excitability of specific populations of RVM neurons and establish the link between these changes and pain behaviors. A better understanding of molecular, cellular, and circuit-level mechanisms that underlie pain is essential if we are to develop better treatments. By carefully mapping the descending projections from PAG to RVM during the development of persistent inflammation, and by tying these to defined RVM outputs and behavior, we can begin to determine the interactions in this complex network, and gain new insights into how pain-modulating systems are recruited and modulated in acute and chronic pain.

项目总结