Clinical platform for high-throughput analyses of extracellular vesicles
细胞外囊泡高通量分析的临床平台
基本信息
- 批准号:10462501
- 负责人:
- 金额:$ 57.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-08-02 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAdoptedAdoptionBenchmarkingBiological AssayBiomedical EngineeringBiopsyBiotechnologyBlood CirculationBlood specimenCancer DetectionCancer DiagnosticsCancer PatientCellsClinicClinicalClinical ResearchConsumptionDana-Farber Cancer InstituteDetectionDevelopmentDevice DesignsDevicesDiagnosisDiagnosticDiseaseDrug resistanceEnrollmentEnsureEnzyme-Linked Immunosorbent AssayEvolutionGeneral HospitalsGoalsGoldHeterogeneityImmunotherapeutic agentIndustryIndustry StandardLaboratoriesLeadMalignant NeoplasmsMalignant neoplasm of ovaryMassachusettsMeasuresMedical DeviceMethodsMicrofabricationMolecularMolecular AnalysisMonitorNatureNucleic AcidsOutcomes ResearchParentsPatientsPeriodicityPharmaceutical PreparationsPlatinumPreparationProductionProtein AnalysisProteinsProtocols documentationQuality ControlRNAReaderReproducibilityResearchResourcesSamplingScreening for Ovarian CancerSignal TransductionStandardizationStructureSurface Plasmon ResonanceSystemSystems BiologyTechniquesTechnologyTestingTherapy trialThinkingTimeTissuesTranslatingTranslationsTreatment EfficacyTreatment outcomeTumor BurdenUltracentrifugationVariantVesicleWestern Blottingbasecancer biomarkerscancer carecancer cellcancer drug resistancecancer therapycirculating biomarkersclinical applicationclinical diagnosticsclinical translationclinically translatabledesigndiagnostic technologiesexosomeextracellular vesicleshigh throughput analysisin-vitro diagnosticsindustry partnerinhibitorinnovationinnovative technologiesinstrumentliquid biopsymultidisciplinarynanoplasmonicnext generationnovelnovel diagnosticspatient oriented researchpersonalized cancer therapyprecision medicinescreeningsensor technologysuccesstooltranslational impacttreatment responsetumortumor heterogeneity
项目摘要
Extracellular vesicles (EVs) have emerged as a promising surrogate for the tissue biopsy, potentially
enabling non-invasive, real-time cancer monitoring. Most cancer cells release large numbers of EVs into
circulation that carry molecular constituents of the parent tumor. Analyzing EVs could thus offer new avenues
to assess tumor burden and tumor heterogeneity. Adoption of EV analyses into a clinical workflow, however, is
impeded by the lack of standardized, practical methods. Conventional assay tools (e.g., ultracentrifugation,
Western blotting, ELISA) require large sample volumes and extensive processing; they are impractical for
clinical applications. Variations in sample handling and testing protocols often lead to inconsistent and variable
findings. The goal of this proposal is i) to address such technical challenges by advancing a robust platform for
EV protein analyses, and ii) to rigorously evaluate EVs' clinical value as cancer biomarkers. We formed a
strategic academic-industry partnership to achieve this goal: Exosome Diagnostics, an industry leader in
EV-based cancer diagnostics, offering ready capacity to develop and manufacture in-vitro diagnostic medical
devices; and the Center for Systems Biology at Massachusetts General Hospital, a pioneer in developing novel
analytical technologies for EV analyses. These teams will bring in their multidisciplinary expertise, innovative
technologies and complementary resources to carry out the following translational projects: First, we will build
a sensitive, high-throughput platform for EV protein screening. The system will adopt our recently developed
nPLEX (nano-plasmonic exosome) technology that is based on novel surface plasmon resonance (SPR)
through nanohole structures. Our initial study showed that nPLEX achieved >1000-fold higher sensitivity than
conventional methods and yet consumed scant sample volumes (0.1 μL). The new nPLEX system will have
expanded analytical capacity and scalability for commercial production. We will design a new SPR chip and a
detection instrument for massively parallel EV screening, and also establish standardized assay protocols.
Leveraging the developmental and regulatory expertise of Exosome Diagnostics, the resulting platform will be
ready for translation into clinical diagnostic laboratories. Second, we will perform a targeted clinical study,
particularly testing whether EV protein signatures can be used as a biomarker for cancer detection and
treatment monitoring. We will collect circulating EVs from ovarian cancer patients undergoing therapies, and
track serial changes of EV protein levels. We will ensure assay reliability and reproducibility to deliver
clinically translatable EV diagnostics. We will follow stringent quality control on device design and sample
processing, accrue well-annotated patient and control samples, and perform multisite testing. The technical
and scientific outcomes of this research could have a significant translational impact in cancer, establishing
a robust, highly specific assay to guide treatment decision and assess therapeutic efficacy. Exosome
Diagnostics will provide regulatory guidance as well as channels to market new tests and devices.
细胞外囊泡(EVs)已成为一种很有前途的组织活检替代物
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Rapid and simple single-chamber nucleic acid detection system prepared through nature-inspired surface engineering.
- DOI:10.7150/thno.57153
- 发表时间:2021
- 期刊:
- 影响因子:12.4
- 作者:Park J;Woo S;Kim J;Lee H;Yoo YE;Hong S
- 通讯作者:Hong S
Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device.
- DOI:10.1038/s41551-020-00654-0
- 发表时间:2020-12
- 期刊:
- 影响因子:28.1
- 作者:Cheong J;Yu H;Lee CY;Lee JU;Choi HJ;Lee JH;Lee H;Cheon J
- 通讯作者:Cheon J
3D tracking of extracellular vesicles by holographic fluorescence imaging.
- DOI:10.1126/sciadv.abc2508
- 发表时间:2020-11
- 期刊:
- 影响因子:13.6
- 作者:Liebel M;Ortega Arroyo J;Beltrán VS;Osmond J;Jo A;Lee H;Quidant R;van Hulst NF
- 通讯作者:van Hulst NF
Precise Nanosizing with High Dynamic Range Holography.
- DOI:10.1021/acs.nanolett.0c03699
- 发表时间:2021-01-13
- 期刊:
- 影响因子:10.8
- 作者:Ortiz-Orruño U;Jo A;Lee H;van Hulst NF;Liebel M
- 通讯作者:Liebel M
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Hakho Lee其他文献
Hakho Lee的其他文献
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{{ truncateString('Hakho Lee', 18)}}的其他基金
High-throughput Phenotyping of iPSC-derived Airway Epithelium by Multiscale Machine Learning Microscopy
通过多尺度机器学习显微镜对 iPSC 衍生的气道上皮进行高通量表型分析
- 批准号:
10659397 - 财政年份:2023
- 资助金额:
$ 57.29万 - 项目类别:
3D Fourier Imaging System for High Throughput Analyses of Cancer Organoids
用于癌症类器官高通量分析的 3D 傅里叶成像系统
- 批准号:
10577796 - 财政年份:2022
- 资助金额:
$ 57.29万 - 项目类别:
3D Fourier Imaging System for High Throughput Analyses of Cancer Organoids
用于癌症类器官高通量分析的 3D 傅里叶成像系统
- 批准号:
10358186 - 财政年份:2022
- 资助金额:
$ 57.29万 - 项目类别:
High-throughput Integrated Magneto-electrochemical Exosome (HiMEX) platform to identify neurodevelopmental markers associated with pre and postnatal oxycodone exposure
高通量集成磁电化学外泌体 (HiMEX) 平台,用于识别与产前和产后羟考酮暴露相关的神经发育标志物
- 批准号:
10017043 - 财政年份:2019
- 资助金额:
$ 57.29万 - 项目类别:
Clinical platform for high-throughput analyses of extracellular vesicles
细胞外囊泡高通量分析的临床平台
- 批准号:
9754806 - 财政年份:2018
- 资助金额:
$ 57.29万 - 项目类别:
Clinical platform for high-throughput analyses of extracellular vesicles
细胞外囊泡高通量分析的临床平台
- 批准号:
10224771 - 财政年份:2018
- 资助金额:
$ 57.29万 - 项目类别:
Clinical platform for high-throughput analyses of extracellular vesicles
细胞外囊泡高通量分析的临床平台
- 批准号:
9906460 - 财政年份:2018
- 资助金额:
$ 57.29万 - 项目类别:
Multiplexed exosome analyses with DNA barcoding
使用 DNA 条形码进行多重外泌体分析
- 批准号:
9266748 - 财政年份:2016
- 资助金额:
$ 57.29万 - 项目类别:
Multiplexed exosome analyses with DNA barcoding
使用 DNA 条形码进行多重外泌体分析
- 批准号:
9099367 - 财政年份:2016
- 资助金额:
$ 57.29万 - 项目类别:
MAGNETIC NANOSENSORS FOR BIOMEDICAL ANALYSES OF MICROVESICLES
用于微泡生物医学分析的磁性纳米传感器
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8458935 - 财政年份:2012
- 资助金额:
$ 57.29万 - 项目类别:
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