Gnotobiology Core

项目摘要/摘要(GNOTOBIOLOGY核心) 灵知生物学(意为“已知生命”)是一门研究没有微生物(无细菌)或 与确定的微生物联合体有关。涉及灵生斑马鱼的实验是每一个 本计划项目资助的研究项目。灵知生物学核心提供必要的专业知识 确保本提案中描述的实验以最高效率和最低限度地进行 不必要地使用脊椎动物。除了确保现有的无菌衍生方法和 灵知菌实验的实施是为了保证最高水平的质量控制， 灵知生物核心将开展研究，扩展我们目前灵知生物畜牧业的极限 方法论。这些研究目标包括延长无菌斑马鱼 可以维持，开发新的方法来共同饲养斑马鱼，以控制斑马鱼的个体间传播 他们的微生物，并增加了可以用斑马鱼进行的实验的范围和类型- 通过在细菌中开发和实施新的基因工程策略来促进相关微生物的发展。 实现这些目标将显著增强执行 研究项目。

PROJECT SUMMARY/ABSTRACT (GNOTOBIOLOGY CORE) Gnotobiology (meaning “known life”) is the science of animals that are devoid of microbes (germ-free) or associated with defined microbial consortia. Experiments involving gnotobiotic zebrafish are central to each of the Research Projects of this Program Project Grant. The Gnotobiology Core provides the expertise necessary to ensure that experiments described in this proposal are carried out with maximal efficiency and minimal unnecessary use of vertebrate animals. Beyond ensuring that established methods of germ-free derivation and gnotobiotic experimentation are implemented to guarantee the highest level of quality control, the Gnotobiology Core will carry out research that extends the limits of our current gnotobiotic husbandry methodologies. These research objectives include extending the period of time over which germ-free zebrafish can be maintained, developing new ways to co-house zebrafish to control the inter-individual transmission of their microbes, and increasing the range and type of experiments that can be performed with zebrafish- associated microbes through developing and implementing new genetic engineering strategies in bacteria. Achieving these objectives will significantly enhance the ability to carry out experiments described in the Research Projects.