Gnotobiology Core

知识生物学核心

• 批准号: 10227104
• 负责人: JUDITH S EISEN
• 金额: $ 29.32万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-06 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10227104
• 关键词: Adolescent; Adult; Animals; Bacteria; Collection; Derivation procedure; Engineering; Ensure; Generations; Genetic Engineering; Germ-Free; Gnotobiotic; Health; Housing; Individual; Knowledge; Larva; Life; Methodology; Methods; Microbe; Mission; Modeling; Program Research Project Grants; Quality Control; Research; Research Project Grants; Science; System; Time; Vertebrates; Zebrafish; bacterial genetics; design; experimental study; genetic manipulation; improved; innovation; microbial; microbiota; success; transmission process

PROJECT SUMMARY/ABSTRACT (GNOTOBIOLOGY CORE) Gnotobiology (meaning “known life”) is the science of animals that are devoid of microbes (germ-free) or associated with defined microbial consortia. Experiments involving gnotobiotic zebrafish are central to each of the Research Projects of this Program Project Grant. The Gnotobiology Core provides the expertise necessary to ensure that experiments described in this proposal are carried out with maximal efficiency and minimal unnecessary use of vertebrate animals. Beyond ensuring that established methods of germ-free derivation and gnotobiotic experimentation are implemented to guarantee the highest level of quality control, the Gnotobiology Core will carry out research that extends the limits of our current gnotobiotic husbandry methodologies. These research objectives include extending the period of time over which germ-free zebrafish can be maintained, developing new ways to co-house zebrafish to control the inter-individual transmission of their microbes, and increasing the range and type of experiments that can be performed with zebrafish- associated microbes through developing and implementing new genetic engineering strategies in bacteria. Achieving these objectives will significantly enhance the ability to carry out experiments described in the Research Projects.

项目总结/摘要（生物学核心） Gnotobiology（意思是“已知的生命”）是一门关于没有微生物（无菌）或 与确定的微生物联合体有关。涉及生物性斑马鱼的实验是每一项研究的核心。 本计划资助的研究项目。Gnotobiology核心提供必要的专业知识 为了确保本提案中描述的实验以最大的效率和最小的成本进行， 不必要地使用脊椎动物。除了确保已建立的无菌衍生方法和 实施gnotobiotic实验，以保证最高水平的质量控制， Gnotobiology Core将开展研究，扩展我们目前的Gnotobiology畜牧业的限制 方法论。这些研究目标包括延长无菌斑马鱼 可以保持，开发新的方法来共同饲养斑马鱼，以控制个体间的传播， 他们的微生物，并增加可以用斑马鱼进行的实验的范围和类型- 通过在细菌中开发和实施新的基因工程策略， 实现这些目标将大大提高进行实验的能力， 研究项目。