CF 1: Biospecimen Repository Core

Cf-1：生物菌素仓库 摘要 Biospecimen信息库核心(CF-1)旨在为基本和翻译 孢子的研究努力。CF-1将在收集、注释、存储、 对参加研究方案的患者的组织和血液生物标本进行分发和跟踪。 核心将为孢子调查人员提供肿瘤样本的组织病理学专家评估 既有来自研究方案登记的患者，也有来自小鼠和异种移植模型的。另外 它将帮助选择适当的福尔马林固定、石蜡包埋组织和速冻组织 研究和试点项目，以及创建用于孢子的组织微阵列(TMA) 调查员研究。核心将在表演和口译方面提供协助 免疫组织化学研究和荧光原位杂交(FISH)检测。它将提供 TMA图像的扫描和数字存档以及IHC的可靠解释、评分和存档 冰冻、石蜡包埋切片或TMA切片的结果。核心还将提供系统性的 在人类组织和肉瘤小鼠模型中对肿瘤治疗的反应的评估，包括 对药物反应的免疫组织化学标志物的解释，如激酶的变化 磷酸化、抑制增殖和诱导细胞凋亡。它还提供了一个集中的 使用IMPACT平台对肉瘤样本和细胞系进行突变注释的资源。 最后，它确保与临床肉瘤数据库的联系，与项目负责人、 生物信息学核心(CF-2 QIN/SOCCI)和管理核心(Singer)。这建立了一个实质性的 并使我们能够以具有成本效益的方式提供所需的服务。CF-1是 与MSKCC机构病理学核心紧密结合(由MSKCC癌症支持 中心核心拨款)和由行政核心管理的临床研究数据库(CRDB)。

CF-1: Biospecimen Repository ABSTRACT The Biospecimen Repository Core (CF-1) is designed to provide support to the basic and translational research efforts of the SPORE. The CF-1 will play a central role in collecting, annotating, storing, distributing, and tracking of tissue and blood biospecimens from patients enrolled in research protocols. The Core will provide SPORE investigators with expert histopathological evaluation of tumor samples both from patients enrolled on research protocols and from murine and xenograft models. Additionally it will assist in selecting appropriate formalin-fixed, paraffin-embedded tissue and snap-frozen tissue for research and pilot projects, as well as enable the creation of tissue microarrays (TMAs) for SPORE investigator studies. The Core will provide assistance in performing and interpreting immunohistochemical studies and fluorescence in situ hybridization (FISH) assays. It will provide scanning and digital archiving of TMA images and reliable interpretation, scoring, and archiving of IHC results from frozen, paraffin-embedded sections or TMA sections. The core will also provide systematic assessment of tumor response to therapy in human tissues and in sarcoma murine models, including interpretation of immunohistochemical markers for drug response, such as changes in kinase phosphorylation, proliferation inhibition, and induction of apoptosis. It also provides a centralized resource for mutational annotation of sarcoma samples and cell lines using the IMPACT platform. Finally, it ensures linkage to the clinical sarcoma database in collaboration with project leaders, the bioinformatics core (CF-2 Qin/Socci) and the administrative core (Singer). This establishes a substantial infrastructure and allows us to provide the required services in a cost-effective manner. The CF-1 is tightly integrated with the MSKCC Institutional Pathology Core (supported by the MSKCC Cancer Center Core Grant) and the Clinical Research Database (CRDB) managed by the administrative core.