C1 - Biospecimen Repository Core

C1 - 生物样本存储库核心

基本信息

批准号：
7976119
负责人：
CRISTINA R ANTONESCU
金额：
$ 7.59万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7976119
关键词：
Animal Model Animals Apoptosis Archives Area Biological Assay Cell Line Clinical Clinical Data Collection Complement Complement component C1s Core Facility DNA Data Databases Diagnosis Dideoxy Chain Termination DNA Sequencing Drug resistance Enrollment Evaluation Excision Fibrosis Formalin Freezing Genotype Goals Housing Human Image Immunohistochemistry In Situ Individual Laboratories Lesion Microscopic Molecular Monitor Morphology Mus Necrosis Needle biopsy procedure Normal tissue morphology Nucleic Acids Oncogenes Paraffin Embedding Pathologic Pharmaceutical Preparations Phosphotransferases Pilot Projects Play Preparation Procedures Protocols documentation Reproduction spores Research Research Personnel Research Project Grants Resources Role Sampling Scanning Serum Soft Tissue Neoplasms Specimen System Tissues Tumor Cell Line Update Xenograft Model chemotherapy design digital human tissue oncology peripheral blood programs repository response sarcoma soft tissue tumor

项目摘要

The Biospecimen Repository Core (CF-A) is designed to provide support to the basic translafional research efforts of the SPORE. The Core will play a central role in collecfing, annotafing, storing, distribufing, and tracking sarcoma and normal tissue blospecimens from pafients enrolled in research protocols. The four key focus areas forthe sarcoma SPORE Biospecimen Repository are high quality banked fissue (frozen and paraffin-embedded) and blood (serum and plasma); accurate and updated procedural, pathologic, and clinical annotafion; efficient and effective distribufion of blospecimens for use in research protocols; and a robust tracking system capable of monitoring blospecimens from collecfion through utilization. Integrating these four components is a major goal of the Biospecimen Repository Core. The Core will provide SPORE invesfigators with expert histopathologic evaluation of tumor samples, both from pafients enrolled on research protocols and from murine and xenograft models. The Core will provide assistance in performing and interprefing immunohistochemical studies, in selecfing fissue for microdissecfion and construcfion of arrays, and in collaborating with project leaders and CF-B. The core will also provide systematic assessment of tumor response to therapy in human tissues and in animal models of sarcomas, including interpretafion of immunohistochemical markers for drug response, such as changes in kinase phosphorylafion, inhibifion of proliferafion, and inducfion of apoptosis. The Core will provide mutafional annotafion for sarcoma tumors and cell lines, as needed by individual research projects.

Biospecimen存储库核心（CF-A）旨在为基本的翻译研究提供支持孢子的努力。核心将在集合，注释，存储，分发和跟踪来自研究方案的能力表的肉瘤和正常的组织Blospecimens。四个键肉瘤孢子生物循环库的焦点区域是高质量银行的Fissue（冷冻和石蜡包裹）和血液（血清和血浆）；准确，更新的程序，病理学和临床Annotafion;有效有效地分布散粒细胞，以用于研究方案；和强大的跟踪系统，能够通过利用来监测collection的Blospecimens。整合这四个组成部分是生物测量存储库核心的主要目标。核心将为孢子的入侵者提供对肿瘤样本的专家组织病理学评估从参与研究方案以及鼠和异种移植模型的能力水平。核心将提供协助执行和插入免疫组织化学研究，在Selecfing Fissue中阵列的微隔离和约束，并与项目负责人和CF-B合作。核心意愿还可以系统地评估人体组织中肿瘤对治疗的反应和动物模型肉瘤，包括对药物反应的免疫组织化学标记的解释，例如变化激酶磷酸化，抑制增殖和凋亡诱导。核心将提供根据各个研究项目的需要，用于肉瘤肿瘤和细胞系的肌肉肿瘤。