C1 - Biospecimen Repository Core

生物样本库核心（CF-A）旨在为SPORE的基础转化研究工作提供支持。核心将在收集、注释、存储、分发和跟踪研究方案中招募的患者的肉瘤和正常组织活检标本方面发挥核心作用。肉瘤孢子生物标本库的四个关键重点领域是高质量的库存组织（冷冻和石蜡包埋）和血液（血清和血浆）;准确和更新的程序，病理和临床注释;用于研究方案的生物标本的高效和有效的分配;以及能够从收集到使用监测生物标本的强大跟踪系统。整合这四个组件是生物标本库核心的主要目标。核心将为SPORE研究者提供肿瘤样本的专家组织病理学评价，这些样本来自研究方案中招募的患者以及小鼠和异种移植模型。核心将在执行和解释免疫组织化学研究，选择组织进行显微切割和阵列的构建，并与项目负责人和CF-B合作方面提供帮助。该核心还将提供对人类组织和肉瘤动物模型中肿瘤对治疗的反应的系统评估，包括对药物反应的免疫组织化学标记物的解释，例如激酶磷酸化的变化，增殖的抑制和凋亡的诱导。该核心将根据个别研究项目的需要，为肉瘤肿瘤和细胞系提供突变注释。

The Biospecimen Repository Core (CF-A) is designed to provide support to the basic translational research efforts of the SPORE. The Core will play a central role in collecting, annotating, storing, distribufing, and tracking sarcoma and normal tissue blospecimens from patients enrolled in research protocols. The four key focus areas for the sarcoma SPORE Biospecimen Repository are high quality banked tissue (frozen and paraffin-embedded) and blood (serum and plasma); accurate and updated procedural, pathologic, and clinical annotation; efficient and effective distribution of blospecimens for use in research protocols; and a robust tracking system capable of monitoring blospecimens from collection through utilization. Integrating these four components is a major goal of the Biospecimen Repository Core. The Core will provide SPORE investigators with expert histopathologic evaluation of tumor samples, both from patients enrolled on research protocols and from murine and xenograft models. The Core will provide assistance in performing and interpreting immunohistochemical studies, in selecting tissue for microdissection and construction of arrays, and in collaborating with project leaders and CF-B. The core will also provide systematic assessment of tumor response to therapy in human tissues and in animal models of sarcomas, including interpretation of immunohistochemical markers for drug response, such as changes in kinase phosphorylation, inhibition of proliferation, and induction of apoptosis. The Core will provide mutational annotation for sarcoma tumors and cell lines, as needed by individual research projects.