Core C: Molecular Targets

摘要 核心C（Chow，Petricoin）分子靶标 分子靶标核心是一个多功能核心。核心提供了一个收集、跟踪、存储和 生物样品的分发点，是进行必要实验室工作的资源和服务 在本申请中提出的分析。此外，分子靶标核心是 计划项目，因为它允许项目以一致的、可复制的和成本- 分子标记鉴定的有效方法。核心将在一个重要的 所有项目和其他核心的方式。此外，核心小组还制定了详细的表格和标准作业程序， 是临床和鼠实验方案以及样品管理的重要资源 一个不断增长的生物标本库。与生物统计学核心（核心B）、分子生物学 目标核心将进行样本分析，并与每个项目就以下内容的转移和/或分析进行互动： 样品，以及对结果的管理和解释。核心提供 免疫组化方法开发和分析。核心还提供反相蛋白 微阵列（RPPA），一种多重定量测量多种信号蛋白，其中许多是 磷蛋白，从生物标本。RPPA代表了一种新的定量测量方法， 一个标本中的数百种蛋白质

ABSTRACT Core C (Chow, Petricoin) Molecular Targets The Molecular Targets Core is a multi-functional core. The Core provides a collection, tracking, storage, and distribution point for biological samples and is a resource and service for carrying out the necessary laboratory analyses proposed in this application. Furthermore, the Molecular Targets Core is an essential component of the Program Project in that it allows the Projects to address Specific Aims in a consistent, reproducible, and cost- effective manner with regards to the identification of molecular markers. The Core will interact in a significant manner with all Projects and the other cores. Additionally, the Core has put into place detailed forms and SOPs, and is an essential resource for development of clinical and murine protocols as well as sample management for a continually growing biospecimen repository. In collaboration with the Biostatistics Core (Core B), Molecular Targets Core will carry-out sample analyses and interact with each Project on the transfer and/or analysis of samples, as well as on the management and interpretation of the results. The Core provides immunohistochemical method development and analysis. The Core also provides Reverse Phase Protein Microarray (RPPA), a multiplexed quantitative measurement of multiple signaling proteins, many of which are phosphoproteins, from biological specimens. RPPA represents a novel means of quantitative measurement of hundreds of proteins from a single specimen.