Core C: Molecular Targets

核心 C：分子靶点

基本信息

批准号：
10015221
负责人：
H-H. Sherry CHOW
金额：
$ 32.25万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-10 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10015221
关键词：
Actinic keratosis Address Apoptotic Architecture Biological Biological Assay Biological Markers Biological Specimen Banks Biopsy Biostatistics Core Cells Clinical Trials Collaborations Collection Exposure to Formalin Freezing Hematoxylin and Eosin Staining Method Histopathology Human Laboratories Light Maps Measurement Molecular Target Mus Paraffin Embedding Pathway interactions Phase Phosphoproteins Prevention Procedures Protein Microarray Assay Protein Microchips Proteins Protocols documentation Reproducibility Resource Development Resources Sampling Services Shipping Signal Transduction Signaling Protein Skin Skin Carcinoma Slide Specimen Stains Standardization Sunburn TLR4 gene The Sun Thick Tissue Microarray clinical development cost cost effective laser capture microdissection method development molecular marker mouse model novel programs sample collection skin squamous cell carcinoma sun damage tissue processing

项目摘要

ABSTRACT Core C (Chow, Petricoin) Molecular Targets The Molecular Targets Core is a multi-functional core. The Core provides a collection, tracking, storage, and distribution point for biological samples and is a resource and service for carrying out the necessary laboratory analyses proposed in this application. Furthermore, the Molecular Targets Core is an essential component of the Program Project in that it allows the Projects to address Specific Aims in a consistent, reproducible, and cost- effective manner with regards to the identification of molecular markers. The Core will interact in a significant manner with all Projects and the other cores. Additionally, the Core has put into place detailed forms and SOPs, and is an essential resource for development of clinical and murine protocols as well as sample management for a continually growing biospecimen repository. In collaboration with the Biostatistics Core (Core B), Molecular Targets Core will carry-out sample analyses and interact with each Project on the transfer and/or analysis of samples, as well as on the management and interpretation of the results. The Core provides immunohistochemical method development and analysis. The Core also provides Reverse Phase Protein Microarray (RPPA), a multiplexed quantitative measurement of multiple signaling proteins, many of which are phosphoproteins, from biological specimens. RPPA represents a novel means of quantitative measurement of hundreds of proteins from a single specimen.

抽象的核心C（Chow，Petricoin）分子靶标分子靶标的核心是多功能核心。核心提供了一个集合，跟踪，存储和生物样品的分配点，是进行必要实验室的资源和服务该应用程序中提出的分析。此外，分子靶标的核心是该计划项目允许这些项目以一致，可重现和成本 - 关于鉴定分子标记的有效方式。核心将在重要的与所有项目和其他核心的方式。此外，核心已将详细的表格和SOP置于并且是开发临床和鼠协议以及样本管理的重要资源对于不断增长的生物测量存储库。与生物统计学核心（核心B）合作，分子目标核心将进行样本分析，并与每个项目进行有关转移和/或分析的互动样本以及结果的管理和解释。核心提供免疫组织化学方法的开发和分析。核心还提供反相蛋白微阵列（RPPA），多重定量测量多种信号蛋白，其中许多是磷蛋白，来自生物标本。 RPPA代表了一种新颖的定量测量方法来自单个标本的数百种蛋白质。