Histology Core

肌肉骨骼组织的机械和生物功能是基于其结构， 组织和组成。维持组织完整性，损伤和修复的急性变化，以及 随着衰老和疾病的进行性和慢性变化，可以在亚细胞上评估和量化， 细胞、细胞外和组织水平。正常和病理人体组织， 动物模型被广泛用于研究这些过程的细胞、细胞外和组织决定因素。 因此，通过标准和高级染色对关键组织学变化进行定性和分析描述 技术，以及人体组织中重要组织形态学参数的评估和测量 动物模型是研究肌肉骨骼健康和疾病关键问题的基础。 本组织学核心的总体目标是开发和应用广泛的标准和 评估肌肉骨骼组织的创新组织学和组织形态计量学方法 结构和组成，并为新项目和合作提供培训和资金 使用这些方法。由于其结构和组成的异质性，肌肉骨骼组织 提出了独特的实验和分析挑战，需要定制的，敏感的和多样化的 组织学测定。PCMD组织学核心提供复杂的专业知识，以及一套标准和 新的工具和技术，这是专门为整个范围的肌肉骨骼组织， 包括大小动物的椎间盘、骨、软骨、纤维软骨、肌腱和肌肉 模型、人体组织样本和组织工程构建体。支持的传统技术 石蜡切片、冰冻切片、塑型、包埋、切片及组织化学染色 如成像和分析，以及更先进和复杂的技术，如免疫组织化学 和组织形态学。其他新的尖端技术将被添加到核心功能套件中， 这次更新。组织学核心还通过以下方式为PCMD成员和学员提供教育计划： 一系列机制，重点是标准和先进的组织学技术，及其应用， 在健康和疾病条件下的肌肉骨骼研究。关于服务 交付，核心提供全面和自助服务的选择，辅以研究设计咨询和数据 最大限度地发挥核心的影响力。一个受欢迎的和完全补贴的协议开发， 为用户提供优化服务，以设想和快速启动新的、特定于应用的组织学 技术.通过这次更新，组织学核心将继续作为一个独特的和不可或缺的 为宾夕法尼亚大学和更广泛的社区的研究人员提供资源，促进创新和高影响 肌肉骨骼研究，并刺激当前和新的PCMD成员之间的合作， 以前没有包括在他们的肌肉骨骼研究计划的组织学方法。

The mechanical and biological functions of musculoskeletal tissues are based on their structure, organization and composition. Maintenance of tissue integrity, acute changes with injury and repair, as well as progressive and chronic changes with aging and disease, can be evaluated and quantified on the sub-cellular, cellular, extracellular, and tissue level by histological techniques. Normal and pathological human tissues and animal models are widely used to study the cellular, extracellular, and tissue determinants of these processes. Thus, qualitative and analytical descriptions of key histological changes by standard and advanced staining techniques, and assessment and measurements of important histomorphological parameters in human tissue and animal models are at the foundation of examining critical questions in musculoskeletal health and disease. The overall objective of this Histology Core is to develop and apply a wide range of standard and innovative histological and histomorphometric approaches to evaluate musculoskeletal tissue structure and composition, and to provide training and funding for new projects and collaborations using these approaches. Due to their heterogeneous structures and compositions, musculoskeletal tissues present unique experimental and analytical challenges that require customized, sensitive and diverse histological assays. The PCMD Histology Core provides sophisticated expertise, and a suite of standard and novel tools and techniques, which are specially tailored for the complete range of musculoskeletal tissues, including intervertebral disc, bone, cartilage, fibrocartilage, tendon and muscle from large and small animal models, human tissue samples, and tissue-engineered constructs. Conventional techniques supported by the core include paraffin, frozen and plastic processing, embedding, sectioning and histochemical staining, as well as imaging and analysis, and more advanced and sophisticated techniques such as immunohistochemistry and histomorphometry. Additional new, cutting edge techniques will be added to the suite of core capabilities in this renewal. The Histology Core also provides educational programs for PCMD members and trainees through a range of mechanisms focusing on standard and advanced histological techniques, and their application to musculoskeletal research across a spectrum of healthy and disease conditions. With respect to service delivery, the core offers both full and self-service options, complemented by study design consultation and data interpretation to maximize the Core’s impact. A popular and fully subsidized protocol development and optimization service is offered to users to envision and jumpstart novel, application-specific histological techniques. Through this renewal, the Histology Core will continue to serve as a unique and indispensable resource for researchers at UPenn and in the wider community, catalyzing innovative and high impact musculoskeletal research, and stimulating collaborations between current and new PCMD members who may have not previously included histological approaches in their musculoskeletal research programs.