High Frame Rate 3-D Super Resolution Ultrasound Microvascular Imaging

高帧率 3D 超分辨率超声微血管成像

• 批准号: 10478978
• 负责人: Paul A Dayton
• 金额: $ 34.78万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-10 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10478978
• 关键词: 3-Dimensional; Acoustics; Address; Anatomy; Angiography; Atherosclerosis; Blood Vessels; Brain; Breast; Buffers; Cancer Detection; Cancer Model; Clinic; Clinical; Clinical Research; Collaborations; Computer software; Contrast Media; Data; Detection; Development; Diagnostic; Dimensions; Engineering; Exhibits; FDA approved; FPS-FES Oncogene; Fingerprint; Frequencies; Hour; Human; Image; Imaging Techniques; Imaging technology; Industrialization; Lesion; Malignant - descriptor; Malignant Neoplasms; Methods; Microscopy; Morphology; Motion; Motivation; Nature; North Carolina; Oncology; Paper; Performance; Prostate; Public Health; Publishing; Rattus; Research Personnel; Resolution; Rodent; Rodent Model; Safety; Scientist; Seminal; Sensitivity and Specificity; Slice; Specificity; System; Techniques; Technology; Testing; Three-Dimensional Image; Three-Dimensional Imaging; Thyroid Gland; Time; Tissues; Transducers; Translating; Ultrasonography; Universities; Work; angiogenesis; base; cancer imaging; clinical application; clinically relevant; clinically translatable; computerized data processing; contrast enhanced; contrast imaging; cost; data acquisition; data exchange; data streams; density; design; design and construction; human disease; human model; image processing; imaging approach; imaging modality; improved; in vivo; innovation; new growth; new technology; next generation; novel; novel imaging technique; novel strategies; optical imaging; portability; preclinical study; rapid growth; sarcoma; standard of care; targeted biomarker; treatment response; tumor; tumor growth; ultrasound; vasa vasorum; vector; wound healing

ABSTRACT Recently, the revolutionary technology of super localization microscopy in the optical imaging domain has been translated into the medical ultrasound domain. By localizing the centers of scattering contrast agents, a similar ultrasound localization microscopy technique, also referred to as contrast enhanced super-resolution (CESR) imaging, has been demonstrated with ultrasound. This novel technique enables imaging of microvessels at resolutions as small as ten micrometers, over an order of magnitude smaller than the ultrasound diffraction limit, and at depths much greater than traditionally limited by frequency. In order to achieve advances in all three of these seeming paradoxical dimensions - super-resolution contrast imaging requires that thousands of frames of data to be analyzed, making this technique much slower than standard ultrasound imaging. The result is that super-resolution imaging would be difficult if not impossible to translate to the clinic in its current form with current clinical hardware, especially if 3-D imaging is desired (which it is for microvascular morphological analysis), as a single 3-D image volume would take tens of minutes to acquire. However, there is a solution to this, which our group proposes to achieve in this project. Recent advances in ultrasound hardware have enabled ultra-high frame rate processing. Our academic and clinical teams at UNC Chapel Hill are partnering with Verasonics, Inc, a world leading industrial partner in next-generation ultrasound systems, to develop and translate the first high-frame rate 3-D super resolution imaging modality to the clinic. We will do this by first designing and constructing a 1024 channel ultra-high frame rate ultrasound system, designed to operate with a 32x32 matrix transducer. Ultra-fast processors, large RAM buffers, GPUs, and high- bandwidth data transfer hardware will be utilized to handle the massive data acquisition and processing. New software and implementation approaches designed at UNC, including our innovative adaptive multi-focus beamforming approach, will further increase sensitivity and resolution at clinically relevant depths, and enable full 3-D volume acquisitions at volume frame rates over 5000 FPS, suitable for fast 3-D super-resolution imaging in humans. Our approach will be validated in phantoms, rodent models of human disease, and in two different clinical applications where ultrasound specificity is limited, breast, and thyroid. Our motivation is to develop super-resolution imaging as a novel new approach for imaging angiogenesis – one of the hallmarks of cancer, as a new biomarker target for both diagnostics and assessment of response to therapy. The ability to differentiate lesions based on microvascular fingerprint, rather than tumor anatomy, would be a paradigm shift in ultrasound diagnostics, and will improve the specificity of ultrasound to malignancy, and advance clinically needed in breast, prostate, thyroid, and other oncological applications. However, the advancement of the proposed technology will undoubtedly open doors to other clinical applications as well, such as wound healing and vasa vasorum imaging in atherosclerosis.

摘要 近年来，光学成像领域中革命性的超局域显微技术 已经被翻译到医学超声领域。通过定位散射造影剂的中心， 一种类似的超声定位显微技术，也称为对比度增强超分辨率 (CESR)成像，已用超声波演示。这项新技术使微血管成像成为可能。 分辨率小到10微米，比超声衍射小一个数量级 其深度远大于传统上受频率限制的深度。为了在这三个方面都取得进展 在这些看似矛盾的维度中-超分辨率对比度成像需要数千帧 这使得这项技术比标准的超声成像要慢得多。结果是， 超分辨率成像将是困难的，如果不是不可能的话，以目前的形式转换到临床上 临床硬件，特别是如果需要3D成像(用于微血管形态分析)，如 一个3D图像体积需要几十分钟才能获取。 然而，有一个解决方案，我们的小组建议在这个项目中实现。最新进展 在超声方面，硬件已经实现了超高帧速率处理。我们在北卡罗来纳大学的学术和临床团队 教堂山正在与Verasonics，Inc.合作，后者是下一代超声波领域的世界领先工业合作伙伴 系统，开发第一个高帧速率三维超分辨率成像设备并将其转化为临床。 我们将通过首先设计和构建1024通道超高帧速率超声系统来实现这一点， 设计为与32x32矩阵传感器一起运行。超高速处理器、大容量RAM缓冲区、GPU和高性能 利用带宽数据传输硬件处理海量数据采集和处理。新的 在北卡罗来纳大学设计的软件和实施方法，包括我们创新的自适应多焦点 波束形成方法，将进一步提高临床相关深度的灵敏度和分辨率，并使 全三维体采集，体帧速率超过5000 FPS，适合快速三维超分辨率成像 在人类身上。我们的方法将在幻影、人类疾病的啮齿动物模型和两种不同的 超声特异度有限的临床应用，乳房和甲状腺。 我们的动机是开发超分辨率成像作为一种新的血管生成成像方法 -癌症的特征之一，作为新的生物标记物目标，用于诊断和评估对 心理治疗。根据微血管指纹而不是肿瘤解剖学来区分病变的能力将 将是超声诊断的范式转变，并将提高超声对恶性肿瘤的特异性，以及 在乳腺、前列腺、甲状腺和其他肿瘤学应用方面的临床需要的进展。然而， 拟议技术的进步无疑也将为其他临床应用打开大门，例如 AS创面愈合和动脉粥样硬化的血管成像。

项目成果

Characterization of the Ultrasound Localization Microscopy Resolution Limit in the Presence of Image Degradation.

存在图像退化时超声定位显微镜分辨率极限的表征。

• DOI: 10.1109/tuffc.2021.3112074
• 发表时间: 2022
• 期刊: IEEE transactions on ultrasonics, ferroelectrics, and frequency control
• 作者: McCall,JacobR;Dayton,PaulA;Pinton,GianmarcoF
• 通讯作者: Pinton,GianmarcoF

Interactive, in-browser cinematic volume rendering of medical images.

医学图像的交互式浏览器内电影体积渲染。

• DOI: 10.1080/21681163.2022.2145239
• 发表时间: 2023
• 期刊: Computer methods in biomechanics and biomedical engineering. Imaging & visualization
• 作者: Xu,Jiayi;Thevenon,Gaspard;Chabat,Timothee;McCormick,Matthew;Li,Forrest;Birdsong,Tom;Martin,Ken;Lee,Yueh;Aylward,Stephen
• 通讯作者: Aylward,Stephen

Longitudinal 3-D Visualization of Microvascular Disruption and Perfusion Changes in Mice During the Evolution of Glioblastoma Using Super-Resolution Ultrasound.

使用超分辨率超声对胶质母细胞瘤进化过程中小鼠的微血管破坏和灌注变化进行纵向 3D 可视化。

• DOI: 10.1109/tuffc.2023.3320034
• 发表时间: 2023
• 期刊: IEEE transactions on ultrasonics, ferroelectrics, and frequency control
• 作者: McCall,JacobR;DeRuiter,Ryan;Ross,Mark;Santibanez,Francisco;Hingtgen,ShawnD;Pinton,GianmarcoF;Dayton,PaulA
• 通讯作者: Dayton,PaulA

Adaptive Multifocus Beamforming for Contrast-Enhanced-Super-Resolution Ultrasound Imaging in Deep Tissue.

用于深层组织对比增强超分辨率超声成像的自适应多焦点波束形成。

• DOI: 10.1109/tuffc.2018.2865903
• 发表时间: 2018
• 期刊: IEEE transactions on ultrasonics, ferroelectrics, and frequency control
• 作者: Espindola,David;Lin,Fanglue;Soulioti,DanaiE;Dayton,PaulA;Pinton,GianmarcoF
• 通讯作者: Pinton,GianmarcoF

Super-resolution Ultrasound Imaging.

• DOI: 10.1016/j.ultrasmedbio.2019.11.013
• 发表时间: 2020-04
• 期刊: Ultrasound in medicine & biology
• 影响因子: 2.9
• 作者: Christensen-Jeffries K;Couture O;Dayton PA;Eldar YC;Hynynen K;Kiessling F;O'Reilly M;Pinton GF;Schmitz G;Tang MX;Tanter M;van Sloun RJG
• 通讯作者: van Sloun RJG