Investigating the regulation of alternative splicing and its role in disease

rna结合蛋白（rbp）在细胞命运决定中的鉴定。为了系统地识别在正常生理和疾病中发挥重要作用的rbp，我们将在干细胞向神经元谱系分化的一系列过程中，将chymera介导的rbp遗传扰动与单细胞中表型丰富的转录组分析结果结合起来。此外，我们将通过联合靶向rbp和阐明复杂的基因调控网络来扩展这些研究。为此，我们将利用CHyMErA基因相互作用作图功能，设计一个库，将针对数百个rbp单独和组合。

Identification of RNA-binding proteins (RBPs) underlying cell fate decisions. To systematically identify RBPs with important roles in normal physiology and disease, we will couple CHyMErA-mediated genetic perturbations of RBPs with phenotypically rich transcriptome profiling read-outs in single cells during a series of stem cell differentiation towards the neuronal lineage. Furthermore, we will extend these studies by combinatorially targeting RBPs and elucidating complex gene regulatory networks. Towards this, we will exploit CHyMErA genetic interaction mapping capabilities and design a library that will target several hundreds of RBPs individually and combinatorially.