Flow Cytometry Training Workshop 2021-22

流式细胞术培训研讨会 2021-22

• 批准号: 10593370
• 负责人: ERICA ANDERSEN-NISSEN
• 金额: $ 2.5万
• 依托单位: FRED HUTCHINSON CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-06 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10593370
• 关键词: Flow; Cytometry; Training; Workshop; 2021

Project Summary Flow cytometry is a specialized technology that characterizes cells on a single-cell basis and is heavily used in the fields of immunology, infectious diseases and cancer biology. As the technology has advanced, the multiplexing capability has markedly expanded allowing for more markers to be examined simultaneously. These advances have increased the complexity of experimental design and of the expertise required to appropriately analyze and detect potential artifacts hidden in the resulting data. Advanced training is critical to allow these technologies to be appropriately implemented in resource-limited settings where exposure to scientists and mentors with the requisite expertise is limited. Although complete mastery can take years, the practical and theoretical tools needed to achieve competency can be taught in a relatively short workshop. The African Flow Cytometry Workshop has been held biennially since 2005 in Cape Town, South Africa, with the aim of enhancing both the theoretical and technical flow cytometry knowledge of African immunologists so that this cutting-edge technology can be applied to critical studies being conducted on the continent. This technology has unique capabilities to help address scientific questions of particular relevance in Africa given the high prevalence of disease caused by the three major global pathogens, HIV, tuberculosis and malaria. As flow cytometry instruments with greater capabilities have become more available in Africa, the number of scientists needing this advanced knowledge has also increased. Training opportunities that exist for African investigators remain very limited and can be very costly. The African Flow Cytometry Workshop is structured over five full days and consists of a combination of lectures and hands-on tutorials with homework assigned each evening and an initial and final exam. Attendance is limited to 20 participants, to ensure appropriate interaction between faculty and students and to facilitate peer-to-peer interactions. We have conducted eight previous workshops and this experience has demonstrated the ongoing need for this type of training as well as shown successful outcomes for many prior participants (Nemes, et al., Cytometry A, 2016). As in prior workshops, the selection process is conducted in a thoughtful way in order to choose participants across geographic regions in Africa who are likely to apply their training and train others in their home laboratory. In a survey we conducted of prior workshop participants, almost 90% of respondents reported having trained peers at their home institution after the workshop, often by adapting teaching materials from the workshop. In an effort to perpetuate the training, we have incorporated a strategy to invite select high- achieving past participants to join the workshop faculty, which has served to deepen their understanding of flow cytometry and provide them with leadership training. The workshop training is therefore maximized through a “train the trainer” model and through the development of a specialized network of African flow cytometry experts in the field of infectious disease.

项目摘要 流式细胞术是在单细胞基础上表征细胞的专门技术，并且被大量用于 免疫学、传染病和癌症生物学领域。随着技术的进步， 多路复用能力已显著扩展，允许同时检查更多的标记。这些 这些进步增加了实验设计的复杂性，也增加了适当地进行实验所需的专业知识的复杂性。 分析和检测隐藏在结果数据中的潜在伪影。高级培训是至关重要的， 在资源有限的环境中适当实施的技术， 具备必要专门知识的导师有限。虽然完全掌握可能需要数年时间，但实际和 可以在一个相对较短的讲习班中教授达到能力所需的理论工具。 非洲流式细胞术研讨会自2005年起每两年在南非开普敦举行一次， 目的是提高非洲免疫学家的理论和技术流式细胞术知识， 这种尖端技术可以应用于在非洲大陆进行的关键研究。这 鉴于非洲的特殊性，技术具有独特的能力来帮助解决与非洲特别相关的科学问题 艾滋病毒、结核病和疟疾这三大全球病原体造成的疾病发病率很高。如流量 在非洲，具有更大能力的细胞仪变得更容易获得， 对这些先进知识的需求也在增加。为非洲调查人员提供的培训机会 仍然非常有限，并且可能非常昂贵。 非洲流式细胞术研讨会为期五天，包括讲座 和实践辅导，每天晚上布置家庭作业，并进行初始和期末考试。出席人数有限 20名参与者，以确保教师和学生之间的适当互动，并促进对等 交互.我们已经举办了八次研讨会，这一经验表明， 需要这种类型的培训，并显示出许多先前参与者的成功结果（Nemes等人， Cytometry A，2016）。与以前的讲习班一样，选择过程是以深思熟虑的方式进行的，以便 选择非洲各地理区域的参与者，他们有可能应用他们的培训并培训其他人， 他们的家庭实验室在我们对之前的研讨会参与者进行的一项调查中，近90%的受访者 报告说，讲习班结束后，他们在本国机构培训了同龄人，通常是改编教材 从车间。为了使培训永久化，我们采取了一项战略，邀请一些高- 让过去的参与者加入研讨会的教师队伍，这有助于加深他们对心流的理解 并为他们提供领导力培训。因此，通过 “培训培训员”模式，并通过发展非洲流式细胞术专家专业网络， 在传染病领域。