Handheld 3D Bioprinting of Self-Healing Hydrogels for Vocal Fold Reconstruction

用于声带重建的自愈水凝胶的手持式 3D 生物打印

• 批准号: 10538010
• 负责人: Amir Kamal Miri Ramsheh
• 金额: $ 6.54万
• 依托单位: NEW JERSEY INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-05 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10538010
• 关键词: Handheld; 3D; Bioprinting; Self; Healing

PROJECT SUMMARY/ABSTRACT Between 3% and 9% of the general population have a voice problem at any given point in time. Such problems have serious psychological, functional and economic consequences, in particular for teachers and professional voice users. The most severe conditions affecting voice are when vocal fold mucosa is lost or replaced by scar. For large mucosa voids or scarring, we propose to deliver effective biomaterials into the vocal fold site using an endoscopy-fit handheld bioprinter. This approach may help regenerate a functional vocal fold tissue and restore voice production. Biomimetic self-healing hydrogels act like fluid flow during needle extrusion and rapidly solidify when the precursors mix, thus allowing in situ deposition of vocal fold implants via extrusion. We will optimize a self-healing hydrogel formulation that adheres and seals quickly to the host tissue, in which phenol-based components will be used as adhesives due to their biocompatibility and tunable adhesion strength. We will assess the biocompatibility of the proposed hydrogel using human vocal fold fibroblasts. The hydrogel will feed a custom-made handheld bioprinter with tunable length and coaxial geometry. We will explore bioprinting strategies to deposit such vocal fold implants via changing design parameters in the handheld bioprinter and extrusion of the proposed hydrogel to vocal fold sites. We will test our handheld bioprinter using ex vivo larynges, and will optimize the deposition parameters. In the last step, we will incorporate an animal model for in vivo assessment of our hydrogel and to justify the clinical efficiency of our proposed handheld bioprinter. Our overarching goal is to translate the proposed self-healing hydrogel system and the bioprinting platform into otolaryngology clinics in the United States when we successfully complete this grant.

项目摘要/摘要 在任何给定的时间点，总人口的3％至9％都有语音问题。这样的 问题具有严重的心理，功能和经济后果，尤其是教师和 专业的语音用户。影响声音的最严重条件是丢失声带粘膜或 用疤痕取代。对于大型粘膜空隙或疤痕，我们建议将有效的生物材料传递到 使用内窥镜拟合手持式生物生物器使用声带折叠位点。这种方法可能有助于再生功能 声带组织并恢复语音产生。仿生自我修复水凝胶的作用像流体流动 当前体混合时，针头挤出并快速凝固，从而可以原位沉积声。 通过挤压植入物。我们将优化一种自我修复的水凝胶配方，该配方迅速粘附和密封 宿主组织，由于其生物相容性，将基于苯酚的组件将其用作粘合剂 和可调的粘附力。我们将使用人类评估所提出的水凝胶的生物相容性 声带折叠成纤维细胞。水凝胶将以可调长度和 同轴几何。我们将探索生物打印策略，以通过更改来存放此类声折叠植入物 在手持生物运输媒体中设计参数，并将提议的水凝胶挤出到声带位点。我们 将使用离体喉部测试我们的手持式生物生产商，并将优化沉积参数。在 最后一步，我们将合并一个动物模型，用于体内水凝胶的体内评估，并证明临床合理 我们提议的手持生物生产商的效率。我们的总体目标是翻译拟议的自我修复 当我们 成功完成这项赠款。