Regulation and Targeting of HIV-1 Integrase-RNA Interactions

HIV-1 整合酶-RNA 相互作用的调控和靶向

• 批准号: 10520066
• 负责人: Sebla B. Kutluay
• 金额: $ 47万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-01-01 至 2026-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10520066
• 关键词: Anti-Retroviral Agents; Antiviral Agents; Binding; Biochemical; Biological Assay; C-terminal; CRISPR screen; Capsid; Cells; Cementation; Clinical; Complement; Complex; Data; Defect; Dependence; Development; Distal; Drug resistance; Electron Microscopy; Electrostatics; Ensure; Enzymes; Evolution; Funding; Generations; Genome; Goals; Guide RNA; HIV-1; Highly Active Antiretroviral Therapy; Human Activities; In Vitro; Innate Immune Response; Integrase; Integrase Inhibitors; Kinetics; Knowledge; Life Cycle Stages; Location; Mediating; Molecular; Morphogenesis; Mutagenesis; Mutation; Nature; Nucleic Acids; Nucleocapsid; Outcome; Pathogenesis; Pathway interactions; Patients; Pharmaceutical Preparations; Play; Publishing; RNA; RNA Binding; RNA Degradation; RNA Recognition Motif; RNA-Directed DNA Polymerase; Regulation; Reporting; Resistance; Reverse Transcription; Role; Sampling; Small Interfering RNA; Specificity; Testing; Therapeutic; Vaccines; Variant; Viral; Viral Genome; Virion; Virus; Virus Replication; Work; empowerment; experimental study; inhibitor; mutant; novel; particle; physical separation; premature; therapeutic development; therapeutic target; tool; viral RNA

Abstract The emergence of drug resistant human immunodeficiency virus type-1 (HIV-1) variants and the lack of an effective vaccine require the development of novel anti-retroviral drugs. The catalytic activity of HIV-1 integrase (IN) has been successfully targeted by several highly effective and well tolerated IN strand transfer inhibitors (INSTIs). However, despite high barriers with the second-generation INSTIs, mutations conferring resistance to multiple INSTIs have been reported in clinical settings. Thus, targeting IN through an alternative mechanism can complement the existing therapeutic strategies and substantially increase the barrier to emergence of drug resistant HIV-1 variants upon INSTI treatment. IN has long been known to have an enigmatic non-catalytic function in the HIV-1 life cycle. Certain mutations in IN, collectively referred to as class II mutations, are reportedly pleiotropic and result in defects in viral particle assembly, maturation and reverse transcription. In a paradigm-shifting study, we have discovered that HIV-1 IN binds to the viral RNA genome (gRNA) in virions and that this interaction is critically important for accurate virion morphogenesis. Inhibition of IN-gRNA interactions through allosteric integrase inhibitors (ALLINIs) or class II IN substitutions results in the formation of aberrant “eccentric” particles with the gRNA is mislocalized between the empty capsid (CA) lattice and the viral envelope. Furthermore, we have shown that IN tetramerization is critical for RNA-binding and that a number of class II IN substitutions located throughout IN inhibit RNA binding through modulation of IN tetramerization. Finally, work from our lab demonstrated that premature degradation of the gRNA and its physical separation from the reverse transcriptase enzyme in virions underlies the reverse transcription defects of eccentric particles in target cells. Importantly, this untimely gRNA degradation is not due to inhibition of IN-gRNA interactions per se, but rather due to loss of protection with the CA lattice, as a similar outcome was observed upon CA destabilization. Together, these studies cemented the role of IN-gRNA interactions in virion maturation and demonstrated the critical importance of the CA lattice in protection of viral nucleic acids in target cells. Based on these novel findings and extensive preliminary data, we propose to elucidate the nature and rules of HIV-1 IN-gRNA interactions, how IN binding to the gRNA mediates proper assembly of the HIV-1 capsid lattice and how infected cells sense and respond to aberrant particles generated upon inhibition of IN-gRNA interactions and destabilization of the CA lattice. These studies will fill a critical gap in our understanding of the critical noncatalytic function of HIV-1 IN in particle maturation and the consequences of inhibiting these interactions. Together, this project will not only enhance our basic knowledge of HIV-1 replication but also aid in the development of novel antivirals that can complement INSTI-based therapies in clinical settings.

摘要