Feasibility study of novel hand hygiene formulations against bacterial spore infection

项目总结： 芽胞形成细菌种类，如各种芽孢杆菌和梭状芽胞杆菌，都会产生孢子。 在不利条件下形成休眠孢子的过程。这些细菌孢子有 对食品工业构成威胁，因为它们会导致食物变质和食物中毒。在……里面 此外，还有一种机会性病原体艰难梭菌(Clostridium Difficile)，它是五种疾控中心之一- 列出的紧急威胁，导致艰难梭菌感染(CDI)在医疗保健和长期 定期护理系统。不幸的是，目前还没有散发性的手部卫生产品。 用肥皂和水洗手是唯一推荐的预防CDI的方法 卫生保健环境或在家里(疾控中心、艰难梭菌：防止艰难梭菌的传播)。这是众所周知的 肥皂和水并不能灭活细菌孢子，而是把它们从皮肤上去除。 进入排水系统。因此，手部卫生方法具有很高的杀孢子活性，但 无毒性，迫切需要更好地保护普通人群免受感染 由芽胞形成细菌引起的。基于专利和正在申请专利的技术， Camellix，LLC开发了含有表没食子儿茶素-3-羟基儿茶素的新型醇基制剂. 没食子酸酯棕榈酸酯(EC16)，一种从绿茶多酚中提取的化合物，显示出强大的 最初测试中的杀孢子活性。在目前的提案中，来自Camellix和Seton的调查人员 霍尔大学将：确定和验证新型酒精类化合物的杀孢子活性 含表没食子儿茶素没食子酸酯棕榈酸酯(EC16)的制剂对广谱的 细菌孢子，并可提供无毒性的新手部卫生方法(目标1)。这个 具有强大杀孢子活性的选定配方将接受细胞毒性测试， 目标1中确定的最终F1和F2与当前使用的稳定性和有效性 方法和产品(目标2)。在NIH STTR第二阶段拨款的支持下，Camellix目前正在 根据EPA、FDA和欧盟法规开发新型手/表面消毒剂和 标准，并取得预期的结果，这表明我们在 手部卫生产品开发。拟议的工作将导致一流的无毒手 抗病原性芽胞形成细菌的卫生原型。

Project Summary: Spore-forming bacterial species, such as various Bacilli and Clostridia, undergo a sporulation process under unfavorable conditions to form dormant spores. These bacterial spores have become a threat to the food industry as they can cause food spoilage and food poisoning. In addition, an opportunistic pathogen, Clostridium difficile (C. difficile), one of the five CDC- listed urgent threats, causes Clostridium difficile infection (CDI) in the healthcare and long- term care systems. Unfortunately, there are currently no sporicidal hand hygiene products. Hand wash with soap and water is the only recommended method to prevent CDI either in healthcare settings or at home (CDC, C. difficile: Prevent the spread of C. difficile). It is known that soap and water do not inactivate bacterial spores, but rather remove them from the skin into the water drainage system. Thus, hand hygiene methods with high sporicidal activity, yet without toxicity, are in urgent need to better protect the general population from infections caused by spore-forming bacteria. Based on patented and patent-pending technologies, Camellix, LLC has developed novel alcohol-based formulations containing epigallocatechin-3- gallate-palmitate (EC16), a compound derived from green tea polyphenols, which showed potent sporicidal activity in initial tests. In the current proposal, investigators from Camellix and Seton Hall University will: determine and validate the sporicidal activity of novel alcohol-based formulations containing epigallocatechin-3-gallate-palmitate (EC16) against a wide spectrum of bacterial spores and can provide novel hand hygiene methods without toxicity (Aim 1). The selected formulations with potent sporicidal activities will be undergoing test the cytotoxicity, stability, and efficacy of finalized F1 and F2 identified in Aim 1 in comparison to currently used methods and products (Aim 2). Supported by an NIH STTR Phase II grant, Camellix is currently developing novel hand/surface disinfectants according to EPA, FDA and EU regulations and standards, and achieving expected results, which demonstrates our expertise and experience in hand hygiene product development. The proposed work will lead to first-in-class nontoxic hand hygiene prototypes against pathogenic spore-forming bacteria.