Modernization of Zebrafish Core Facility

斑马鱼核心设施的现代化

• 批准号: 10541090
• 负责人: Teresa V Bowman
• 金额: $ 38.8万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10541090
• 关键词: Adolescent; Adult; Aging; Animals; Biological; Biological Models; Biology of Aging; Biomedical Research; Breeding; Cancer Biology; Cell Culture Techniques; Chemicals; Complement; Complex; Core Facility; Custom; Development; Diet; Disease; Disease model; Dose; Drug Screening; Embryo; Embryonic Development; Ensure; Eye; Fertility; Fostering; Gases; Genes; Genetic; Genotype; Growth; Health; Hematopoiesis; Hepatology; Housing; Human; Immune response; Infrastructure; Institution; Knowledge; Life Support Systems; Maintenance; Malignant Neoplasms; Medicine; Microinjections; Modeling; Modernization; Modification; Monitor; Names; Nephrology; Neurosciences; Optics; Organism; Pathway interactions; Process; Production; Public Health; Reproducibility; Research; Research Personnel; Resolution; Scientist; Services; System; Toxicology; Transgenic Organisms; United States; United States National Institutes of Health; Update; Whole Organism; Xenograft procedure; Zebrafish; biological systems; cancer cell; clinical application; college; cost; dietary; drug testing; experimental study; feeding; flexibility; human disease; human model; in vivo; in vivo Model; interest; intravital imaging; mutant; novel; prevent; programs; real-time images; screening; translational study; tumor; tumor immunology

PROJECT ABSTRACT: Modernization of Zebrafish Core Facility The Zebrafish Core Facility at Albert Einstein College of Medicine (Einstein) provides investigators access to utilize zebrafish as an in vivo model for a variety of applications such as disease modeling, drug testing and screening, and whole organism intravital imaging. The facility currently offers services to maintain established transgenic and mutant zebrafish lines, provide 100’s of embryos per week, and to rapidly produce new transgenic or mutant animal strains. The first aquatic system of the facility housed was established in 2001 and is still in usage today. Much has changed in zebrafish maintenance and husbandry standards in the field. Thus, we have identified a major need to upgrade this aging system. We plan to update to a state-of-the-art life support system with pH, conductivity, and total dissolved gas monitoring and dosing systems, rotating microscreen drum filter and centralized sump, and new tanks with locking lids to prevent escaping zebrafish. In addition, zebrafish research programs have significantly expanded and diversified since 2001. Our current facility is poorly outfitted to expand into some of these new scientific directions. We have identified a need to provide Einstein researchers with access to technological advances to enable them to enrich their research programs in a variety of directions. The proposed addition of three stand-alone housing systems that permit environmental modifications unique to each housing system will provide added flexibility for new experimental approaches. For example, using zebrafish as personalized avatars for human cancer cell xenografts will allow rapid in vivo drug screening to identify tumor-specific vulnerabilities. Other examples are the exploration of how different diets impact immune responses and cancer growth or in vivo toxicology. The three new stand-alone systems with customized tanks and environmental controls proposed in the application will allow the facility users to perform these specialized experiments without impacting the larger breeding zebrafish colony housed in the main system. These advances will greatly expand the services available to Einstein zebrafish users.

项目摘要：斑马鱼核心设施的现代化 爱因斯坦医学院的斑马鱼核心设施为研究人员提供了 将斑马鱼作为体内模型用于各种应用，如疾病建模、药物测试和 筛查和整个生物体活体成像。该设施目前提供的服务是维护已建立的 转基因和突变斑马鱼品系，每周提供100个S胚胎，并迅速产生新的转基因 或突变的动物品系。该设施的第一个水上系统于2001年建立，目前仍在 今天的使用量。斑马鱼的维护和田间饲养标准发生了很大变化。因此，我们有 确定了升级这一老化系统的主要需求。我们计划升级到最先进的生命维持系统 配有pH、电导率和总溶解气体监测和添加系统，旋转微筛滤筒过滤器 和中央水池，以及带锁盖的新水箱，以防止斑马鱼逃脱。此外，斑马鱼 自2001年以来，研究项目显著扩大和多样化。我们目前的设施装备很差。 以扩展到其中一些新的科学方向。我们已经确定了为爱因斯坦研究人员提供 有机会获得技术进步，使他们能够在不同的方向丰富他们的研究方案。 建议增加三个独立的住房系统，以允许对 每个住房系统都将为新的试验方法提供更大的灵活性。例如，使用 斑马鱼作为人类癌细胞异种移植的个性化化身，将允许快速体内药物筛选 识别特定于肿瘤的漏洞。其他的例子是探索不同的饮食如何影响免疫力。 反应和癌症生长或体内毒理学。三个新的独立系统，带有定制的油箱 申请中提出的环境控制将允许设施用户执行这些专门的 在不影响主系统中更大的斑马鱼繁殖群体的情况下进行试验。这些进展 将极大地扩展爱因斯坦斑马鱼用户可用的服务。