Modernization of Zebrafish Core Facility

斑马鱼核心设施的现代化

• 批准号: 10541090
• 负责人: Teresa V Bowman
• 金额: $ 38.8万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10541090
• 关键词: Adolescent; Adult; Aging; Animals; Biological; Biological Models; Biology of Aging; Biomedical Research; Breeding; Cancer Biology; Cell Culture Techniques; Chemicals; Complement; Complex; Core Facility; Custom; Development; Diet; Disease; Disease model; Dose; Drug Screening; Embryo; Embryonic Development; Ensure; Eye; Fertility; Fostering; Gases; Genes; Genetic; Genotype; Growth; Health; Hematopoiesis; Hepatology; Housing; Human; Immune response; Infrastructure; Institution; Knowledge; Life Support Systems; Maintenance; Malignant Neoplasms; Medicine; Microinjections; Modeling; Modernization; Modification; Monitor; Names; Nephrology; Neurosciences; Optics; Organism; Pathway interactions; Process; Production; Public Health; Reproducibility; Research; Research Personnel; Resolution; Scientist; Services; System; Toxicology; Transgenic Organisms; United States; United States National Institutes of Health; Update; Whole Organism; Xenograft procedure; Zebrafish; biological systems; cancer cell; clinical application; college; cost; dietary; drug testing; experimental study; feeding; flexibility; human disease; human model; in vivo; in vivo Model; interest; intravital imaging; mutant; novel; prevent; programs; real-time images; screening; translational study; tumor; tumor immunology

PROJECT ABSTRACT: Modernization of Zebrafish Core Facility The Zebrafish Core Facility at Albert Einstein College of Medicine (Einstein) provides investigators access to utilize zebrafish as an in vivo model for a variety of applications such as disease modeling, drug testing and screening, and whole organism intravital imaging. The facility currently offers services to maintain established transgenic and mutant zebrafish lines, provide 100’s of embryos per week, and to rapidly produce new transgenic or mutant animal strains. The first aquatic system of the facility housed was established in 2001 and is still in usage today. Much has changed in zebrafish maintenance and husbandry standards in the field. Thus, we have identified a major need to upgrade this aging system. We plan to update to a state-of-the-art life support system with pH, conductivity, and total dissolved gas monitoring and dosing systems, rotating microscreen drum filter and centralized sump, and new tanks with locking lids to prevent escaping zebrafish. In addition, zebrafish research programs have significantly expanded and diversified since 2001. Our current facility is poorly outfitted to expand into some of these new scientific directions. We have identified a need to provide Einstein researchers with access to technological advances to enable them to enrich their research programs in a variety of directions. The proposed addition of three stand-alone housing systems that permit environmental modifications unique to each housing system will provide added flexibility for new experimental approaches. For example, using zebrafish as personalized avatars for human cancer cell xenografts will allow rapid in vivo drug screening to identify tumor-specific vulnerabilities. Other examples are the exploration of how different diets impact immune responses and cancer growth or in vivo toxicology. The three new stand-alone systems with customized tanks and environmental controls proposed in the application will allow the facility users to perform these specialized experiments without impacting the larger breeding zebrafish colony housed in the main system. These advances will greatly expand the services available to Einstein zebrafish users.

项目摘要：斑马鱼核心设施的现代化 阿尔伯特·爱因斯坦医学院（爱因斯坦）的斑马鱼核心设施为研究人员提供了访问 利用斑马鱼作为体内模型用于各种应用，例如疾病建模、药物测试和 筛查和全生物体活体成像。该设施目前提供服务，以维持既定的 转基因和突变斑马鱼系，每周提供100个胚胎，并迅速产生新的转基因斑马鱼。 或突变的动物品系。该设施的第一个水生系统建于2001年，至今仍在运行。 今天的使用在该领域，斑马鱼的维护和饲养标准发生了很大变化。因此，我们有 确定了升级这一老化系统的主要需求。我们计划升级到最先进的生命维持系统 带pH值、电导率和总溶解气体监测和计量系统，旋转微筛转鼓过滤器 和集中式水池，以及带锁盖的新水箱，以防止斑马鱼逃跑。此外，斑马鱼 自2001年以来，研究项目已显着扩展和多样化。我们现在的设施装备很差 扩展到这些新的科学方向。我们已经确定需要为爱因斯坦研究人员提供 获得技术进步，使他们能够在各个方向丰富他们的研究计划。 建议增加三个独立的住房系统，允许环境改造， 每一种住房制度都将为新的试验办法提供更多的灵活性。例如使用 斑马鱼作为人类癌细胞异种移植物的个性化化身将允许快速体内药物筛选， 识别肿瘤特异性脆弱性。其他的例子是探索不同的饮食如何影响免疫力， 反应和癌症生长或体内毒理学。三个新的独立系统与定制的坦克 应用中提出的环境控制将允许设施用户执行这些专门的 在不影响容纳在主系统中的较大的繁殖斑马鱼群体的情况下进行实验。这些进步 将大大扩展爱因斯坦斑马鱼用户可用的服务。