Maintenance and Incidence of ME/CFS following Mono

摘要 这一建议将使我们能够比较EBV和SARS-CoV-2免疫标志物和代谢组学在 来自我们独特数据集的样本。免疫和新陈代谢分析是有趣的发现工具和 我们将使用的综合方法的重要方面，具有广泛和灵活的研究潜力 病毒感染后的疲劳。我们还表明，患有IM后ME/CFS的大学生具有炎症性 个人资料，即使在基线时，也不同于那些从IM中恢复的人(Jason，Cotler等，2021)。此外, 代谢组学已经成为分析病毒后疲劳的一种新工具。多项研究报告称 与能量产生、氨基酸、核苷酸、氮、 病毒后疲劳患者的血脂和神经递质(Armstrong等人，2014；Germain，Ruppert， Levine和Hanson，2017；Nagy-Szakal等人，2018；Naviaux等人，2016)。我们的免疫学研究和 代谢组学希望确定PASC和ME/CFS的可能生物特征。我们的主要目标是 确定SARS CoV-2后PASC发生的免疫学和代谢危险因素 IM后感染与ME/CFS我们假设，改变的宿主免疫和代谢特征将是 与初次EBV和SARS-CoV-2感染后的病毒后疲劳相关，与健康对照 控制。此外，定性的深度访谈将探索人们对以下方面的看法：主要生活 就业状况的变化，躯体症状的变化，应对策略的变化， 身体和精神状态的变化，以及支持系统的演变，以确定哪些 这些因素对发展和维持PASC和ME/CFS也很重要。

Abstract This proposal would allow us to compare EBV and SARS-CoV-2 immune markers and metabolomics on the samples from our unique data sets. Immune and metabolomic analyses are interesting discovery tools and an important aspect of the integrative approach we will use, with their broad and flexible potential for studying post-viral fatigue. We have also shown that college students with post-IM ME/CFS have an inflammatory profile, even at baseline, different from those who recover from IM (Jason, Cotler, et al., 2021). In addition, metabolomics has emerged as a novel tool for analyzing post-viral fatigue. Multiple studies have reported detectable changes in metabolic pathways related to energy production, amino acids, nucleotides, nitrogen, lipids, and neurotransmitters in patients with post-viral fatigue (Armstrong et al., 2014; Germain, Ruppert, Levine, & Hanson., 2017; Nagy-Szakal et al., 2018; Naviaux et al., 2016). Our study of immunology and metabolomics hopes to identify possible biosignatures for PASC and ME/CFS. Our primary aims are to determine immunologic and metabolomic risk factors for the development of PASC following SARS CoV-2 infection and ME/CFS following IM. We hypothesize that altered host immune and metabolomic profiles will be associated with post-viral fatigue following primary EBV and SARS-CoV-2 infection, in contrast to healthy controls. In addition, qualitative in-depth interviews will explore perceptions of the following: major life changes, fluctuations in employment status, changes in somatic symptoms, changes in coping strategies, changes in physical and mental status, and the evolution of support systems, to determine which if any of these factors are also important for the development and maintenance of PASC and ME/CFS.