A novel method for volumetric oxygen mapping in living retina
一种在活体视网膜中进行体积氧测绘的新方法
基本信息
- 批准号:10597011
- 负责人:
- 金额:$ 49.2万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-03-01 至 2025-02-28
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAddressAffectAge related macular degenerationAmericanBiochemistryBlood VesselsBlood capillariesBlood flowCardiovascular systemCell RespirationChoroidConsumptionDataData SetDetectionDiabetic RetinopathyDyesEyeFluorescence AngiographyFundusFutureGlaucomaGoalsHumanHypoxiaImageImaging DeviceImaging TechniquesInhalationLabelLasersLightLocationMachine LearningMapsMeasurementMeasuresMediatingMedicineMetabolicMethodologyMethodsMicroscopyMultimodal ImagingMusNobel PrizeOphthalmoscopesOphthalmoscopyOptical Coherence TomographyOpticsOxygenOxygen saturation measurementPartial PressurePathologicPathologyPhotonsPhysiologyPublic HealthResolutionRetinaRetinal DiseasesRoleScanningSignal PathwaySignal TransductionSpeedTimeTissuesTrainingTranslatingVascular SystemVisible Radiationadaptive opticsalgorithm trainingchoroidal circulationclinical applicationclinical translationdeep learningdesignhemodynamicshuman imagingimaging modalityimprovedin vivolearning networklearning strategylensmachine learning frameworkmultidisciplinarynovelnovel imaging techniqueoptical imagingphosphorescenceporphyrin aprogramsquantitative imagingresponseretina circulationretinal imagingsensorsuccesstooltwo photon microscopytwo-photon
项目摘要
PROJECT SUMMARY
It is widely accepted that oxygen deficiency is a culprit and a marker of several major retinal diseases,
including diabetic retinopathy, age-related macular degeneration, glaucoma etc. However, it remains to be
extremely challenging to measure oxygen in vivo in the eye, and no tools currently exist that can provide 3D
oxygen distributions in the retina with high spatial resolution at appropriate imaging speeds. The goal of this
project is to overcome these limitations and develop a new optical imaging technique for volumetric oxygen
mapping in retina. Our approach will leverage the recently developed potent oxygen probes (such as Oxyphor
2P), whose phosphorescence decay times serve as quantitative markers of local oxygen partial pressures (pO2)
in living tissues. To enable volumetric imaging with high throughput, we propose to develop a novel imaging
instrument, termed oblique scanning laser ophthalmoscope (oSLO). oSLO is based on the concept of single
lens scanned light sheet microscopy and enables volumetric phosphorescence lifetime imaging without time-
consuming plane-by-plane pixel-wise sectioning. Our new method should be able to achieve 10 kilohertz voxel
rate that is three orders of magnitude higher than the current state-of-the-art two-photon phosphorescence
lifetime microscopy (2PLM). In this project we will: (Aim 1) develop a phosphorescence lifetime-based oSLO
for volumetric pO2 mapping in living retina in mouse. The new design will allow parallel detection of signals at
depth from each scanned location, so that the need in conventional depth sectioning is eliminated and imaging
throughput is greatly increased. We will (Aim 2) dynamically image responses of retina and choroid to systemic
hypoxia challenge using Oxyphor 2P. We will (Aim 3) then bridge oSLO measurements and label-free
applications by multimodal imaging with visible light optical coherence tomography (vis-OCT). Using vascular
pO2 as the ground-truth, we will develop a deep spectral training (DSL) algorithm to supervise the inverse
calculation of vis-OCT for robust and reliable label-free retinal oximetry. This study will enable the first direct
quantitative imaging of interactions between the two circulatory systems in retina (i.e. retinal and choroidal
circulation), providing unprecedented information about retinal oxygen supply. IMPACT ON PUBLIC HEALTH:
Successful completion of this program will deliver a new ground-breaking methodology for mapping oxygen in
the retina that will greatly improve our understanding of retinal diseases.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Ji Yi其他文献
Ji Yi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Ji Yi', 18)}}的其他基金
Advancing visible light optical coherence tomography in glaucoma detection
推进可见光光学相干断层扫描在青光眼检测中的应用
- 批准号:
10567788 - 财政年份:2023
- 资助金额:
$ 49.2万 - 项目类别:
Mesoscopic microscopy for ultra-high speed and large-scale volumetric brain imaging
用于超高速和大规模脑体积成像的介观显微镜
- 批准号:
10634911 - 财政年份:2023
- 资助金额:
$ 49.2万 - 项目类别:
A novel method for volumetric oxygen mapping in living retina
一种在活体视网膜中进行体积氧测绘的新方法
- 批准号:
10361407 - 财政年份:2021
- 资助金额:
$ 49.2万 - 项目类别:
Sensitive detection of malignancy in primary acquired melanosis by advanced optical imaging
通过先进光学成像灵敏检测原发性获得性黑变病的恶性肿瘤
- 批准号:
10251585 - 财政年份:2020
- 资助金额:
$ 49.2万 - 项目类别:
Predictive markers for diabetic retinopathy via quantitative imaging of retinal capillary functions
通过视网膜毛细血管功能的定量成像预测糖尿病视网膜病变的标志物
- 批准号:
10461105 - 财政年份:2018
- 资助金额:
$ 49.2万 - 项目类别:
Predictive markers for diabetic retinopathy via quantitative imaging of retinal capillary functions
通过视网膜毛细血管功能的定量成像预测糖尿病视网膜病变的标志物
- 批准号:
10220441 - 财政年份:2018
- 资助金额:
$ 49.2万 - 项目类别:
Predictive markers for diabetic retinopathy via quantitative imaging of retinal capillary functions
通过视网膜毛细血管功能的定量成像预测糖尿病视网膜病变的标志物
- 批准号:
10250563 - 财政年份:2018
- 资助金额:
$ 49.2万 - 项目类别:
相似海外基金
Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
- 批准号:
MR/S03398X/2 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
Fellowship
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
- 批准号:
2338423 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
Continuing Grant
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
- 批准号:
EP/Y001486/1 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
Research Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
- 批准号:
MR/X03657X/1 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
- 批准号:
2348066 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
- 批准号:
AH/Z505481/1 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
EU-Funded
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
Standard Grant
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
- 批准号:
AH/Z505341/1 - 财政年份:2024
- 资助金额:
$ 49.2万 - 项目类别:
Research Grant