CURE AND PREVENTION OF RAT GLIOMA AND HEPATOCARCINOMA
CURE AND PREVENTION OF RAT GLIOMA AND HEPATOCARCINOMA
批准号:
2100536
负责人:
JOSEPH ILAN
金额:
$26.46万
依托单位国家:
美国
项目类别:
财政年份:
1994
资助国家:
美国
项目状态:
已结题
起止时间:
1994-02-15 至 1996-11-30
关键词:
SCID mouse antisense nucleic acid cancer prevention cell type glioma growth factor receptors hepatocellular carcinoma human tissue immune system immunocytochemistry insulinlike growth factor laboratory rat neoplasm /cancer immunology neoplasm /cancer radiation therapy neoplasm /cancer therapy neoplastic cell nonhuman therapy evaluation plasmids transfection
中文摘要
本项目考虑了诱导免疫原性参与
英文摘要
This project considers the involvement of induced immunogenicity in the
cure and prevention of rat glioblastoma in the brain as well as the cure
and prevention of hepatocarcinoma. We plan to inject parental glioma C6
cells into rat brain. After the tumor is established, transfected cells
expressing insulin-like growth factor-I (IGF-I) antisense RNA will be
injected either directly into the brain tumor or subcutaneously at a
distal site. Such treatment cures experimentally established subcutaneous
and brain glioblastoma tumors via an immune response. Therefore, brain
tumors will be analyzed by immunocytochemistry for the identification of
the immune system cell types participating in the cure in three additional
rodent glioblastoma cell lines with an established tumors in the syngeneic
animal in order to determine the generality of the phenomenon. The
possibility that irradiated cells (incapable of division) can activate the
immune system in other cell lines as they do with C6 cells will be studied
with all additional cell lines. Glioma cells transfected with antisense
vector blocking the expression of IGF type-I receptor will be generated
and injected to test whether the receptor participates in an intracrine
mechanism(s) underlying tumorigenicity and immunogenicity. The
interrelationships between the expression of IGF-I and the major
histocompatibility complex-I (MHC-I) will be followed. Other participants
in immune recognition, such as cell surface and soluble co-stimulatory
molecules, will be assessed. Severe Combined Immune Deficiency (SCID)
mice will be implanted with patients lymphocytes in mice carrying
glioblastoma tumor of the same donor. This system will he used to study
immunogenicity of transfected tumor cells expressing antisense IGF-I cDNA.
The second model system, hepatocell carcinoma (generated by the BRL-3A
cell line) will also be studied. These cells express IGF-II as does fetal
liver. They will be transfected with antisense episome-based IGF-II
plasmid and the tumorigenicity and ability to involve the immune response
in vivo will be studied. The potential role of IGF receptors in altered
tumor cell immunogenicity will be evaluated through antisense gene
transfer by a double transfection with IGF type-I and type-II receptors.
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GENE THERAPY FOR BRAIN TUMORS USING ANTISENSE CDNA TRANSCRIPTION GROW
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批准号:6305342
-
项目类别:
-
资助金额:$1.92万
-
财政年份:1999
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负责人:JOSEPH ILAN
-
依托单位:
GENE THERAPY FOR BRAIN TUMORS USING ANTISENSE CDNA TRANSCRIPTION GROW
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批准号:6264402
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项目类别:
-
资助金额:$1.92万
-
财政年份:1998
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负责人:JOSEPH ILAN
-
依托单位:
GENE THERAPY FOR BRAIN TUMORS USING ANTISENSE CDNA TRANSCRIPTION GROWTH FACTOR
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批准号:6246468
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项目类别:
-
资助金额:$1.98万
-
财政年份:1997
-
负责人:JOSEPH ILAN
-
依托单位:
CURE AND PREVENTION OF RAT GLIOMA AND HEPATOCARCINOMA
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批准号:2100535
-
项目类别:
-
资助金额:$25.44万
-
财政年份:1994
-
负责人:JOSEPH ILAN
-
依托单位:
CURE AND PREVENTION OF RAT GLIOMA AND HEPATOCARCINOMA
-
批准号:2100537
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项目类别:
-
资助金额:$27.67万
-
财政年份:1994
-
负责人:JOSEPH ILAN
-
依托单位:
EFFECTS OF IGF EXPRESSION ON FETAL GROWTH
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批准号:3325933
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项目类别:
-
资助金额:$18.63万
-
财政年份:1988
-
负责人:JOSEPH ILAN
-
依托单位:
EFFECTS OF IGF EXPRESSION ON FETAL GROWTH
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批准号:3325930
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项目类别:
-
资助金额:$18.96万
-
财政年份:1988
-
负责人:JOSEPH ILAN
-
依托单位:
EFFECTS OF IGF EXPRESSION ON FETAL GROWTH
-
批准号:3325932
-
项目类别:
-
资助金额:$17.91万
-
财政年份:1988
-
负责人:JOSEPH ILAN
-
依托单位:
EFFECTS OF IGF EXPRESSION ON FETAL GROWTH
-
批准号:3325934
-
项目类别:
-
资助金额:$19.37万
-
财政年份:1988
-
负责人:JOSEPH ILAN
-
依托单位:
EFFECTS OF IGF EXPRESSION ON FETAL GROWTH
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批准号:3325931
-
项目类别:
-
资助金额:$17.85万
-
财政年份:1988
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负责人:JOSEPH ILAN
-
依托单位:
GENE THERAPY FOR TUMORS USING ANTISENSE CDNA TRANSCRIPTION GROWTH FACTOR I
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批准号:3741194
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:JOSEPH ILAN
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依托单位:
海外基金