ELECTRO-IMMUNOSENSOR FOR REGULATING/ACHIEVING FERTILITY

需要廉价的测量装置，其提供 免疫特异性和电化学调节便利性 或获得生育能力。为此，我们建议制定和 评估结合以下优点的新电极试纸设计： 免疫测定和电化学响应。总的目标是 开发一种电化学家用免疫测定法， 不同的荷尔蒙 第一阶段的工作证明了使用这种双重分析物的可行性， 无分离固相电化学酶免疫分析检测 促卵泡激素（FSH）和促黄体生成激素（LH）。 第二阶段的研究将把这种免疫测定法发展成商业化的 无试剂测试条。 拟议的设备包括两个主要的 元件：（1）具有导电性的一次性测试条， 含有吸附的免疫组分的层，和（2）可重复使用的 用于数据分析和信号读出的电子元件。 的 预期的结果是一种廉价的酶免疫测定电极， 生育率监测，根据耦合的原则， 免疫化学反应对电极的反应。 提交这份提案的三位研究人员合作 成功地开发了原型酶基生物传感器技术， 将为本报告所述的发展提供重要的准备。 提议 多分析物免疫传感器将提供额外的， 免费的信息，使这些设备将有一个独特的 商业优势。

There is a need for inexpensive measurement devices that provide immunological specificity and electrochemical convenience for regulating or achieving fertility. For this purpose, we propose to develop and evaluate a new electrode test strip design that combines the advantages of immunoassay and electrochemical response. The general objective is to develop an electrochemical home immunoassay capable of detecting two different hormones. The Phase I work proved the feasibility of using this dual-analyte, separation-less, solid-phase electrochemical enzyme immunoassay to detect follicle stimulating hormone (FSH) and luteinizing hormone (LH) in urine. Phase II research will develop this immunoassay into a commercial reagentless test strip. The proposed device consists of two major elements: (1) a disposable test strip having an electrical conducting layer containing adsorbed immuno-components, and (2) the reusable electronic components used for data analysis and signal readout. The expected result is an inexpensive enzyme immunoassay electrode for fertility monitoring, based on the principle of coupling the immunochemical reaction to the electrode response. The three researchers submitting this proposal have collaborated successfully to develop prototype enzyme-based biosensor technology which will provide crucial preparation for the development described in this proposal. Multi-analyte immunosensors will provide additional, complimentary information so that these devices will have a distinct commercial advantage.