ELECTRO-IMMUNOSENSOR FOR REGULATING/ACHIEVING FERTILITY

需要廉价的测量设备来提供 免疫学特异性和电化学调节便利性 或实现生育能力。为此，我们建议开发和 评估一种新的电极测试条设计，该设计结合了以下优点 免疫测定和电化学反应。总体目标是 开发一种电化学家用免疫测定法，能够检测两种 不同的激素。 第一阶段的工作证明了使用这种双分析物的可行性， 无需分离的固相电化学酶免疫分析法来检测 尿液中的卵泡刺激素（FSH）和黄体生成素（LH）。 II 期研究将把这种免疫测定法开发成商业产品 无试剂试纸条。 所提出的装置由两个主要部分组成 元件：（1）具有导电性的一次性测试条 含有吸附的免疫成分的层，以及（2）可重复使用的 用于数据分析和信号读出的电子元件。 的 预期结果是一种廉价的酶免疫测定电极 生育力监测，基于耦合原理 对电极反应的免疫化学反应。 提交该提案的三位研究人员进行了合作 成功开发原型酶基生物传感器技术 将为本文所述的发展提供重要的准备 提案。 多分析物免疫传感器将提供额外的、 补充信息，使这些设备具有独特的 商业优势。

There is a need for inexpensive measurement devices that provide immunological specificity and electrochemical convenience for regulating or achieving fertility. For this purpose, we propose to develop and evaluate a new electrode test strip design that combines the advantages of immunoassay and electrochemical response. The general objective is to develop an electrochemical home immunoassay capable of detecting two different hormones. The Phase I work proved the feasibility of using this dual-analyte, separation-less, solid-phase electrochemical enzyme immunoassay to detect follicle stimulating hormone (FSH) and luteinizing hormone (LH) in urine. Phase II research will develop this immunoassay into a commercial reagentless test strip. The proposed device consists of two major elements: (1) a disposable test strip having an electrical conducting layer containing adsorbed immuno-components, and (2) the reusable electronic components used for data analysis and signal readout. The expected result is an inexpensive enzyme immunoassay electrode for fertility monitoring, based on the principle of coupling the immunochemical reaction to the electrode response. The three researchers submitting this proposal have collaborated successfully to develop prototype enzyme-based biosensor technology which will provide crucial preparation for the development described in this proposal. Multi-analyte immunosensors will provide additional, complimentary information so that these devices will have a distinct commercial advantage.