ELECTRO-IMMUNOSENSOR TO DETECT COCAINE & THC METABOLITE

提供免疫特异性的测量装置， 电化学方便大规模药物测试以评估 需要对各种工作场所药物滥用战略产生影响。 我们提出 评估和开发一种新的试纸设计， 免疫分析和电化学响应的优点。 总 目的是开发一种电化学免疫测定法， 检测小（半抗原大小）抗原。 第一阶段目标的成功实现证明了 固相电化学酶免疫分析检测 尿液中的药物代谢物。 第二阶段将把这种免疫测定法发展成一种 市售无试剂测试条。 所提出的装置由两个 主要元件：（1）具有电连接器的一次性测试条， 含有吸附的免疫组分的导电层，和（2） 用于从测试条读取信号的电子部件。 的 预期的结果是用于药物使用诊断的酶免疫测定电极， 基于将免疫化学反应偶联到 电极响应 提交这份提案的三位研究人员合作 成功地开发了原型酶基生物传感器技术， 将是中所述研究和开发的关键组成部分， 这个提议。 实用电化学传感器专业 可卡因和四氢大麻酚在人体尿液中的代谢产物将具有巨大的商业价值。 作为简单廉价药物使用的可行性， 公共安全或其他高风险活动（例如，操作汽车， 公共交通和军事硬件）。

Measurement devices that provide immunological specificity and electrochemical convenience for large-scale drug testing to assess the impact of various work place drug abuse strategies are needed. We propose to evaluate and develop a new test strip design that combines the advantages of immunoassay and electrochemical response. The general objective is to develop an electrochemical immunoassay capable of detecting small (haptensized) antigens. The successfully achieved Phase I objective proved the principle of separationless, solid-phase electrochemical enzyme immunoassays to detect drug metabolites in urine. Phase II will develop this immunoassay into a commercial reagentless test strip. The proposed device consists of two major elements: (1) a disposable test strip having an electrical conducting layer containing adsorbed immuno-components, and (2) the electronic components used to read the signal from the test strip. The expected result is an enzyme immunoassay electrode for drug use diagnosis, based on the principle of coupling the immunochemical reaction to the electrode response. The three researchers submitting this proposal have collaborated successfully to develop prototype enzyme-based biosensor technology which will be a crucial component for the research and development described in this proposal. Practical electrochemical sensors for the major metabolites of cocaine and THC in human urine will have great commercial viability as simple inexpensive drug use screens of personnel engaged in public safety or other high-risk activities (e.g., operating automobiles, public transportation, and military hardware).