ELECTRO-IMMUNOSENSOR TO DETECT COCAINE & THC METABOLITE

测量设备，提供免疫学特异性和 用于大规模药物检测评估的电化学方便性 各种工作场所药物滥用的影响策略是必要的。我们建议 评估和开发一种新的测试条设计，该设计结合了 免疫分析和电化学反应的优点。这位将军 目的是建立一种电化学免疫分析方法，能够 检测小的(半抗原)抗原。 第一阶段目标的成功实现证明了 无需分离的固相电化学酶免疫分析法检测 尿液中的药物代谢物。第二阶段将把这项免疫分析发展成 商用无试剂试纸。建议的装置由两个 主要元素：(1)一种一次性试纸，带有一个电路板 包含吸附的免疫成分的导电层，以及(2) 用于从测试条读取信号的电子元件。这个 预期结果是一种用于药物使用诊断的酶免疫分析电极， 基于将免疫化学反应耦合到 电极响应。 提交这项提案的三名研究人员已经合作 成功开发出基于酶的生物传感器原型技术 将是中描述的研究和开发的关键组件 这项提议。实用型主要电化学传感器 人体尿液中的可卡因和THC代谢物将具有很大的商业价值 生存能力作为从事简单廉价药物使用筛查的人员 公共安全或其他高风险活动(例如，操作汽车， 公共交通和军事硬件)。

Measurement devices that provide immunological specificity and electrochemical convenience for large-scale drug testing to assess the impact of various work place drug abuse strategies are needed. We propose to evaluate and develop a new test strip design that combines the advantages of immunoassay and electrochemical response. The general objective is to develop an electrochemical immunoassay capable of detecting small (haptensized) antigens. The successfully achieved Phase I objective proved the principle of separationless, solid-phase electrochemical enzyme immunoassays to detect drug metabolites in urine. Phase II will develop this immunoassay into a commercial reagentless test strip. The proposed device consists of two major elements: (1) a disposable test strip having an electrical conducting layer containing adsorbed immuno-components, and (2) the electronic components used to read the signal from the test strip. The expected result is an enzyme immunoassay electrode for drug use diagnosis, based on the principle of coupling the immunochemical reaction to the electrode response. The three researchers submitting this proposal have collaborated successfully to develop prototype enzyme-based biosensor technology which will be a crucial component for the research and development described in this proposal. Practical electrochemical sensors for the major metabolites of cocaine and THC in human urine will have great commercial viability as simple inexpensive drug use screens of personnel engaged in public safety or other high-risk activities (e.g., operating automobiles, public transportation, and military hardware).