ELECTRO-IMMUNOSENSOR FOR REGULATING/ACHIEVING FERTILITY

描述(改编自申请人的摘要)：主要目的 这个第一阶段的提案是开发和测试一种条状生物传感器电极 可检测尿液中两种不同的抗原(黄体生成素和卵泡刺激素)。该项目 是基于这个小组已经建立的几项技术(酶 技术研究集团，Inc.或ETRG)。首先，这个组织开创了 胶体金固定化抗人血吸虫抗体的技术 电极表面没有失去生物活性。第二，这个群体 已经确定一种抗体与马萝卜过氧化物酶偶联时 暴露在合适的衬底上会产生电子流， 可以通过传感电极进行测量。第三，这个小组将测试 通过使用两种不同的抗体-酶结合物 对于不同的工作电势，测量两个可能是可行的 通过改变氧化还原电位在溶液中分析，而不需要额外的 分析物分离。因此，在典型的反应中，抗原将结合到 固定化抗体-电极表面，第二抗体偶联到 一种酶将夹心抗原，它的存在是固定的 底物浓度越高，就会产生电流。该组织的活动 第二个抗体偶联酶将依赖于氧化还原电位 这个系统。 该生物传感器的原型是一个电化学池，它由一个工作的 涂有含有胶体的导电层的电极 金吸附抗黄体生成素和卵泡刺激素抗体，结合马的卵泡刺激素抗体 萝卜过氧化物酶，黄体生成素抗体偶联葡萄糖氧化酶，底物 两种酶，一个参比电极和一个对电极。这个 商用生物传感器将由一次性测试条组成，该测试条具有 含有被吸收的免疫成分的导电层，以及 用于测试条信号读出的电子元件。

DESCRIPTION (Adapted from the applicant's abstract): The main objective of this Phase I proposal is to develop and test a strip biosensor electrode which can detect two different antigens (LH and FSH) in urine. The project is based on several technologies already established by this group (Enzyme Technology Research Group, Inc. or ETRG). First, this group has pioneered the technique of using colloidal gold to immobilize antibody to an electrode surfaced without loss of biological activity. Second, this group has established that an antibody coupled to horse radish peroxidase when exposed to the appropriate substrate will generate an electron flow that can be measured by a sensing electrode. Third, this group will test the concept that with the use of two different antibody-enzyme conjugates each with different working potentials, it may be feasible to measure two analytes in solution by altering the redox potential without additional analyte separation.Thus, in a typical reaction, the antigen will bind to the immobilized antibody-electrode surface, the second antibody coupled to an enzyme will sandwich the antigen, and it the presence of a fixed substrate concentration, a current will be generated. The activity of the second antibody coupled enzyme will be dependent on the redox potential of the system. The prototype biosensor is an electrochemical cell consisting of a working electrode coated with an electrical conducting layer containing colloidal gold adsorbed antibodies to LH and FSH, FSH antibody coupled to horse radish peroxidase, LH antibody coupled to glucose oxidase, substrates for both enzymes, a reference electrode, and a counter electrode. The commercial biosensor will consist of a disposable test strip having an electrical conducting layer containing absorbed immunocomponents, and the electronic components used for the test strip signal readout.