ELECTRO-IMMUNOSENSOR FOR REGULATING/ACHIEVING FERTILITY

描述（改编自申请人的摘要）：主要目的 第一阶段的建议是开发和测试一种条形生物传感器电极， 其可以检测尿液中的两种不同抗原（LH和FSH）。项目 基于该小组已经建立的几种技术（酶 技术研究集团或ETRG）。 首先，这个组织开创了 用胶体金标记抗人 电极表面无生物活性损失。 第二，这个群体 已经确定，当辣根过氧化物酶与抗体偶联时， 暴露于适当的衬底将产生电子流， 可以通过传感电极测量。 第三，该小组将测试 使用两种不同的抗体-酶缀合物， 在不同的工作电位下，测量两个 通过改变氧化还原电位， 因此，在典型的反应中，抗原将结合至分析物分离。 固定的抗体电极表面，第二抗体偶联到 一种酶会将抗原夹在中间， 当底物浓度增加时，会产生电流。 的活性 第二抗体偶联酶将依赖于 the system. 原型生物传感器是一种电化学电池， 涂覆有导电层的电极，所述导电层包含胶体 LH和FSH金吸附抗体，FSH抗体偶联马 萝卜过氧化物酶，LH抗体偶联葡萄糖氧化酶，底物 两种酶、参比电极和反电极。 的 商业生物传感器将由一次性测试条组成， 含有吸收的免疫成分的导电层， 用于测试条信号读出的电子部件。

DESCRIPTION (Adapted from the applicant's abstract): The main objective of this Phase I proposal is to develop and test a strip biosensor electrode which can detect two different antigens (LH and FSH) in urine. The project is based on several technologies already established by this group (Enzyme Technology Research Group, Inc. or ETRG). First, this group has pioneered the technique of using colloidal gold to immobilize antibody to an electrode surfaced without loss of biological activity. Second, this group has established that an antibody coupled to horse radish peroxidase when exposed to the appropriate substrate will generate an electron flow that can be measured by a sensing electrode. Third, this group will test the concept that with the use of two different antibody-enzyme conjugates each with different working potentials, it may be feasible to measure two analytes in solution by altering the redox potential without additional analyte separation.Thus, in a typical reaction, the antigen will bind to the immobilized antibody-electrode surface, the second antibody coupled to an enzyme will sandwich the antigen, and it the presence of a fixed substrate concentration, a current will be generated. The activity of the second antibody coupled enzyme will be dependent on the redox potential of the system. The prototype biosensor is an electrochemical cell consisting of a working electrode coated with an electrical conducting layer containing colloidal gold adsorbed antibodies to LH and FSH, FSH antibody coupled to horse radish peroxidase, LH antibody coupled to glucose oxidase, substrates for both enzymes, a reference electrode, and a counter electrode. The commercial biosensor will consist of a disposable test strip having an electrical conducting layer containing absorbed immunocomponents, and the electronic components used for the test strip signal readout.