CARRAGEENAN-STABILIZED COLLOIDAL GOLD BIOSENSOR

胶体金固定化卡拉胶包被的酶 电流型生物传感器电极的一条重要新途径。我们的 目的是通过胶体沉积的方法制备这些电极 将含有该酶的金放在导电材料的表面，例如 作为碳，然后附着一层薄薄的卡拉胶。 生物电极可用于生产第二代安培计 非常适合满足人类健康主要需求的生物传感器- 关心。用于检测胆固醇等物质的可靠电流型生物传感器 将在预防、诊断和治疗中得到广泛应用 疾病的治疗。当前第一代产品的主要问题 商用生物传感器设计包括不稳定性、响应速度慢和 相互之间缺乏直接、有效的耦合所造成的干扰 生物活性成分和换能器。我们成功的技术 基于使用卡拉胶为基础的载体材料和胶体 黄金固定化酶克服了目前设计和开发的局限性 为新型电流型生物传感器提供了一条可行的途径。酶技术 研究小组，INS.将进行总体设计的研究和开发， 电极的制备和生物催化性能。一位公爵 大学研究小组将协助评估 电极的电化学性质。

Enzymes immobilized on colloidal gold and coated with carrageenan offer an important new route to amperometric biosensor electrodes. Our objective is to fabricate these electrodes by deposition of colloidal gold containing the enzyme onto the surface of a conducting material such as carbon, followed by the attachment of a thin layer of carrageenan. Bioelectrodes can be used to produce second generation amperometric biosensors that are well suited to satisfy major needs in human health- care. Reliable amperometric biosensors for substances such as cholesterol will find extensive applications in the prevention, diagnosis and treatment of diseases. Major problems with current first generation commercial biosensor designs include instability, slow response, and interferences caused by the lack of direct, efficient coupling between the bioactive component and the transducer. Our successful technology based on the use of carrageenan based support materials and colloidal gold immobilized enzymes overcomes limitations of current designs and offers a viable route to new amperometric biosensors. Enzyme Technology Research Group, Ins., will do research and development on overall design, fabrication, and biocatalytic properties of the electrodes. A Duke University research team will assist in the evaluation of the eletrochemical properties of the electrodes.