CARRAGEENAN-STABILIZED COLLOIDAL GOLD BIOSENSOR

卡拉胶稳定胶体金生物传感器

• 批准号: 3507884
• 负责人: JOHN P O'DALY
• 金额: $ 25.07万
• 依托单位: ALDERON BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-15 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3507884
• 关键词: biomedical equipment development; biosensor device; carrageenan; catalyst; cholesterol; clinical biomedical equipment; colloids; electrochemistry; electrodes; enzyme mechanism; fluorescence spectrometry; gold; oxidoreductase; peroxidases

Enzymes immobilized on colloidal gold and coated with carrageenan offer an important new route to amperometric biosensor electrodes. Our objective is to fabricate these electrodes by deposition of colloidal gold containing the enzyme onto the surface of a conducting material such as carbon, followed by the attachment of a thin layer of carrageenan. Bioelectrodes can be used to produce second generation amperometric biosensors that are well suited to satisfy major needs in human health- care. Reliable amperometric biosensors for substances such as cholesterol will find extensive applications in the prevention, diagnosis and treatment of diseases. Major problems with current first generation commercial biosensor designs include instability, slow response, and interferences caused by the lack of direct, efficient coupling between the bioactive component and the transducer. Our successful technology based on the use of carrageenan based support materials and colloidal gold immobilized enzymes overcomes limitations of current designs and offers a viable route to new amperometric biosensors. Enzyme Technology Research Group, Ins., will do research and development on overall design, fabrication, and biocatalytic properties of the electrodes. A Duke University research team will assist in the evaluation of the eletrochemical properties of the electrodes.

酶固定在胶体金上，并用卡拉胶包被， 一个重要的新路线安培生物传感器电极。我们 目的是通过沉积胶体来制造这些电极 将含有酶的金沉积到导电材料的表面上， 作为碳，然后附着一层薄薄的角叉菜胶。 生物电极可用于产生第二代安培 非常适合满足人类健康主要需求的生物传感器- 在乎用于胆固醇等物质的可靠电流型生物传感器 将在预防、诊断和 治疗疾病。当前第一代的主要问题 商业生物传感器设计包括不稳定、响应慢， 由于缺乏直接的、有效的耦合， 生物活性成分和换能器。我们成功的技术 基于使用角叉菜胶基支持材料和胶体 金固定化酶克服了当前设计的局限性， 提供了一个可行的路线，以新的安培生物传感器。酶技术 研究小组，会做整体设计的研发， 电极的制造和生物催化性能。一个杜克 大学研究小组将协助评估 电极的电化学性质。