IMMOBILIZED OXIDOREDUCTASE ENZYMES IN ORGANIC SYNTHESIS

有机合成中的固定化氧化还原酶

• 批准号: 3508047
• 负责人: JOHN P O'DALY
• 金额: $ 24.1万
• 依托单位: ALDERON BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3508047
• 关键词: alcohol dehydrogenase; bioreactors; catalyst; chemical synthesis; drug design /synthesis /production; gel; method development; nicotinamide adenine dinucleotide; nonwater solvent; organic chemicals; oxidoreductase; stereoisomer

DESCRIPTION: (Adapted from applicant's abstract) There is a need for new reagents for the production of optically pure pharmaceuticals. For this purpose, the investigators propose to develop for commercialization the new biphasic biocatalyst system for organic synthesis for which feasibility was successfully established in the Phase I work. The general objective is to develop the Phase I methodology in which cofactor dependent enzymes were used in organic solvents for the stereospecific production of high value compounds such as pharmaceuticals. The Phase I results demonstrated that horse liver alcohol dehydrogenase (HLADH), along with its cofactor nicotinamide adenine dinucleotide (NAD(H)), can be immobilized in a hydrogel, and operate in a bulk organic solvent to produce optically pure compounds from optically inactive precursors. The specific objective of Phase II will be to develop this new synthetic methodology into a general procedure for the low cost production of pharmaceutical and agricultural compounds using immobilized cofactor dependent oxido-reductase enzymes in organic solvents. The expected result is a commercially viable prototype immobilized oxido- reductase catalyst capable of operating in a bulk organic solvent. The three researchers submitting this proposal have collaborated successfully to establish the feasibility of an immobilized, cofactor-dependent, enzyme system for stereoselective hydrolysis and transesterification in organic solvent.

描述：（改编自申请人摘要）需要新的 用于生产光学纯药物的试剂。 为此 为了达到这个目的，研究人员建议开发商业化的 用于有机合成的新型两相生物催化剂体系， 在第一阶段的工作中成功地建立了可行性。 的 总体目标是制定第一阶段的方法， 在有机溶剂中使用辅因子依赖性酶， 高价值化合物的立体特异性生产， 大药厂 I期结果表明，马肝乙醇脱氢酶 （HLADH），沿着其辅因子烟酰胺腺嘌呤二核苷酸 NAD（H））可以固定在水凝胶中，并且在本体中操作。 用有机溶剂从光学纯化合物中制备光学纯化合物 无活性前体。 第二阶段的具体目标是 将这种新的合成方法发展成一种通用的方法， 低成本生产药用和农用化合物， 有机溶剂中固定化辅因子依赖性氧化还原酶 溶剂. 预期的结果是一个商业上可行的原型固定氧化- 能够在本体有机溶剂中操作的还原酶催化剂。 的 三位提交该提案的研究人员成功地合作， 为了建立一种固定化的，辅因子依赖的， 用于立体选择性水解和酯交换的酶系统， 有机溶剂