IMMOBILIZED FATTY ACIDS FOR REVERSIBLE ALBUMIN BINDING

描述：修订后的第一阶段申请建议评估四个 光活化脂肪酸类似物和三种光活化C16烷基 链衍生物因其能够衍生出四种代表性的 生物材料并随后吸附人白蛋白。 提案清单 四个具体目标。在第一个特定目标中，四个光激活 脂肪酸类似物和三个光活化C16烷基链 衍生物将被化学合成。 在具体目标 2 中，每个 上述化合物将在四种生物材料上进行评估，包括 硅橡胶、聚氨酯、聚乙烯和聚氯乙烯 关于它们在这些生物材料上的负载密度。 下一个 具体目标将解决每种物质的白蛋白吸附特性 上述涂层生物材料与非衍生化生物材料相比 生物材料。最后，每种衍生的生物材料将被 检查其 (1) 吸附血浆蛋白，(2) 粘附的能力 血小板，(3) 支持血小板活化。后面这些研究将 阐明离子基团和疏水基团是否都是必要的 对于观察到的白蛋白结合的增加以及是否增加 白蛋白结合部分归因于亲水性 聚乙二醇间隔基。

DESCRIPTION: This revised Phase I application proposes to evaluate four photoactivatable fatty acid analogs and three photoactivatable C16 alkyl chain derivatives for their ability to derivatize to four representative biomaterials and subsequently adsorb human albumin. The proposal lists four Specific Aims. In the first Specific Aim, four photoactivatable fatty acid analogs and three photoactivatable C16 alkyl chain derivatives will be chemically synthesized. In Specific Aim 2, each of the above compounds will be evaluated on four biomaterials including silicon rubber, polyurethane, polyethylene and polyvinyl chloride with respect to their loading densities on these biomaterials. The next Specific Aim will address the albumin adsorptive properties of each of the above coated biomaterials in comparison to non-derivatized biomaterials. Finally, each of the derivatized biomaterials will be examined for their abilities to (1) adsorb plasma proteins, (2) adhere platelets, and (3) support platelet activation. These latter studies will shed light on whether both ionic and hydrophobic groups are necessary for the observed increase in albumin binding and whether the increase in albumin binding is due, in part, to the use of a hydrophilic polyethylene glycol spacer.