IMMOBILIZED FATTY ACIDS FOR REVERSIBLE ALBUMIN BINDING

描述：此修订后的第一阶段申请建议评估四个 可光活化的脂肪酸类似物和三种可光活化的C16烷基 链衍生物的能力，衍生为四个代表性的 生物材料并随后吸附人白蛋白。 提案列出 四个具体目标。在第一个具体目标中，四个可光活化的 脂肪酸类似物和三个可光活化的C16烷基链 将化学合成衍生物。 在具体目标2中， 上述化合物将在四种生物材料上进行评价， 硅橡胶、聚氨酯、聚乙烯和聚氯乙烯， 关于它们在这些生物材料上的负载密度。 下一 Specific Aim将讨论每种蛋白的白蛋白吸附特性， 与未衍生的生物材料相比 生物材料。最后，每种衍生生物材料将被 检查其（1）吸附血浆蛋白，（2）粘附 血小板，和（3）支持血小板活化。这些研究将 阐明了离子基团和疏水基团是否都是必要的 对于观察到的白蛋白结合增加以及白蛋白结合增加是否 在白蛋白结合中，部分是由于使用亲水性 聚乙二醇间隔物。

DESCRIPTION: This revised Phase I application proposes to evaluate four photoactivatable fatty acid analogs and three photoactivatable C16 alkyl chain derivatives for their ability to derivatize to four representative biomaterials and subsequently adsorb human albumin. The proposal lists four Specific Aims. In the first Specific Aim, four photoactivatable fatty acid analogs and three photoactivatable C16 alkyl chain derivatives will be chemically synthesized. In Specific Aim 2, each of the above compounds will be evaluated on four biomaterials including silicon rubber, polyurethane, polyethylene and polyvinyl chloride with respect to their loading densities on these biomaterials. The next Specific Aim will address the albumin adsorptive properties of each of the above coated biomaterials in comparison to non-derivatized biomaterials. Finally, each of the derivatized biomaterials will be examined for their abilities to (1) adsorb plasma proteins, (2) adhere platelets, and (3) support platelet activation. These latter studies will shed light on whether both ionic and hydrophobic groups are necessary for the observed increase in albumin binding and whether the increase in albumin binding is due, in part, to the use of a hydrophilic polyethylene glycol spacer.