IMMOBILIZED FATTY ACIDS FOR REVERSIBLE ALBUMIN BINDING

描述：这一修订后的第一阶段申请建议评估四个 光活化脂肪酸类似物和三个光活化C16烷基 链衍生品因其衍生能力而具有四个代表性 生物材料，然后吸附人类白蛋白。建议书清单 四个具体目标。在第一个具体目标中，四个可光激活 脂肪酸类似物和三个可光活化的C16烷链 衍生品将通过化学合成。在具体目标2中，每个目标 上述化合物将在四种生物材料上进行评估，包括 硅橡胶、聚氨酯、聚乙烯和聚氯乙烯 考虑到它们在这些生物材料上的装载密度。下一个 具体目标将解决每个白蛋白的吸附性能 上述涂层生物材料与未衍生生物材料的比较 生物材料。最后，每一种衍生生物材料都将 检查它们的能力：(1)吸附血浆蛋白，(2)黏附 血小板；(3)支持血小板活化。这些后一项研究将 阐明离子基团和疏水基团是否都是必要的 对于观察到的白蛋白结合增加以及是否增加 在白蛋白结合中，部分是由于使用了亲水性 聚乙二醇间隔物

DESCRIPTION: This revised Phase I application proposes to evaluate four photoactivatable fatty acid analogs and three photoactivatable C16 alkyl chain derivatives for their ability to derivatize to four representative biomaterials and subsequently adsorb human albumin. The proposal lists four Specific Aims. In the first Specific Aim, four photoactivatable fatty acid analogs and three photoactivatable C16 alkyl chain derivatives will be chemically synthesized. In Specific Aim 2, each of the above compounds will be evaluated on four biomaterials including silicon rubber, polyurethane, polyethylene and polyvinyl chloride with respect to their loading densities on these biomaterials. The next Specific Aim will address the albumin adsorptive properties of each of the above coated biomaterials in comparison to non-derivatized biomaterials. Finally, each of the derivatized biomaterials will be examined for their abilities to (1) adsorb plasma proteins, (2) adhere platelets, and (3) support platelet activation. These latter studies will shed light on whether both ionic and hydrophobic groups are necessary for the observed increase in albumin binding and whether the increase in albumin binding is due, in part, to the use of a hydrophilic polyethylene glycol spacer.