RAPID EIA FOR COTININE IN BIOLOGICAL FLUIDS

烟草制品的消费及其对健康的所有有害影响 我们民众的总体健康，过去是，现在仍然是一个主要的健康问题， 问题. 虽然吸烟的人数 在过去的十年里，吸烟者使用更多的香烟， 青少年不仅消费香烟， 无烟烟草 人们还关注的是， 被动吸烟对非吸烟者，如儿童和青少年， 工作场所和家里。 由于这些问题， 美国政府为获得 无烟社会的目标，这将需要具体的分析 监测和评价这些努力是否成功的程序。 多种方法可用于特异性和敏感性 烟草中尼古丁及其主要代谢物可替宁的测定 生物液体 大多数可用的测定（即，HPLC、GC、RIA） 需要昂贵的设备、训练有素的人员和实验室环境。 我们建议开发一种酶免疫测定法（EIA），这将是有用的 用于检测生物制剂中不同浓度的可替宁 流体. 该测定可适用于监测以下物质的作用： 烟草制品对儿童发育的影响以及 吸烟在民众中的地位。 该方法简便、快速（小于5 分钟），非仪器，因此易于便携式使用， 工作场所或医生办公室。

The consumption of tobacco products with all its deleterious effects upon the general health of our populace, was and still is a major health problem. Although the number of people consuming cigarettes has decreased over the last decade, smokers are using more cigarettes and adolescents are consuming not only cigarettes but also large quantities of smokeless tobacco. There is also concern with regard to effects of passive smoke on non-smokers such as children and adolescents both in the workplace and in the home. Because of these problems, there is a considerable effort being put forth by the U.S. Government to obtain the goal of a smoke-free society which will require specific analytical procedures to monitor and evaluate the success of such efforts. A variety of methods are available for the specific and sensitive determination of nicotine and its major metabolite, cotinine, in biological fluids. Most of the available assays (i.e., HPLC, GC, RIA) require expensive equipment, trained personnel, and a laboratory setting. We propose to develop an enzyme immunoassay (EIA) which will be useful for the detection of various concentrations of cotinine in biological fluids. This assay can be adapted for use in monitoring the effects of tobacco products on child development and for epidemiological studies on smoking status in the populace. The assay is simple, fast (less than 5 minutes), noninstrumental, and thus easily portable for use in the workplace or physicians office.