RAPID ENZYME IMMUNOASSAY FOR CANNABINOIDS IN BLOOD

人们的注意力主要集中在以下人群使用非法药物的问题上： 美国生活的方方面面。 最受欢迎的药物之一， 被我们社会滥用了二十多年的是大麻。 这种药物正在 被来自我们社会各个阶层的人们所使用， 白领工人，军人，专业运动员，以及 高中生 由于这个问题，许多公司，包括 美国军方现在开始要求涉嫌 吸毒者要接受药检。 多种方法可用于特异性和灵敏度测定 大麻及其主要代谢产物的含量 大部分 可用的分析（即，HPLC，GC-MS），需要昂贵的设备，经过培训 人员，并在确定大麻素是否已被消耗方面进展缓慢。 我们建议开发一种酶免疫测定法（EIA）， 检测血液中低浓度的主要大麻素。 通过手指针刺获得的血液可以直接通过我们的分析进行分析， 消除可能的样品污染。 这种定性分析将 作为一个很好的屏幕可能滥用大麻，但所有 阳性样本需要由更敏感和 定量分析（即，GC-MS）。 该方法简便、快速（小于10 分钟），非仪器性的，因此易于便携式的工作中使用 地方

Much attention has been focused on the use of illicit drugs by people from all facets of American life. One of the most popular drugs which has been abused for over two decades by our society is marijuana. The drug is being used by people from all segments of our society including blue and white-collar workers, military personnel, professional athletes, and by high school students. Because of this problem many companies, including the U.S. Military, are now beginning to demand that employees suspected of drug use undergo drug testing. A variety of methods are available for specific and sensitive determination of marijuana and its major metabolites in biological fluids. Most of the available assays (i.e., HPLC, GC-MS), require expensive equipment, trained personnel, and are slow in determining if cannabinoids have been consumed. We propose to develop an enzyme immunoassay (EIA) which will be useful for the detection of low concentrations of the major cannabinoids in blood. Blood obtained by a fingerprick can be annalyzed directly by our assay thus eliminating possible sample contamination. This qualitative assay would serve as an excellent screen for possible cannabinoid abuse, but all positive samples would require verification by more sensitive and quantitative assays (i.e., GC-MS). The assay is simple, fast (less than 10 minutes), noninstrumental and thus easily portable for use in the work place.