Streptomyces bacteria: Antibiotic production in the wheat endosphere

链霉菌:小麦内圈的抗生素生产

基本信息

  • 批准号:
    BB/T015446/1
  • 负责人:
  • 金额:
    $ 64.04万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2020
  • 资助国家:
    英国
  • 起止时间:
    2020 至 无数据
  • 项目状态:
    已结题

项目摘要

Plants use photosynthesis to fix carbon dioxide from the air into glucose which they use as food. However, up to 40% of the carbon they fix is released into the soil from their roots and this appears to be a mechanism to attract beneficial bacteria from the surrounding soil. The bacteria use the root exudates as food and this helps the plants assemble a healthy microbiome. Some of these microbes protect the plants against disease and others help them get important nutrients from the soil. The root microbiome is therefore essential for plant health, but crop breeding over hundreds of years has selected traits such as bigger plants with more grain while perhaps neglecting the (unknown) effects on the microbiome. There is now growing interest in manipulating the microbiomes of crop plants such as wheat to make them more resistant to disease and to abiotic stresses like drought and salinity. Wheat is a staple crop for about 4 billion people and one of the most important crops in the world so increasing yields is essential if we are to support a growing world population. Our project focuses on a genus of soil bacteria called Streptomyces. The ~600 known species of Streptomyces make numerous secondary metabolites, and these account for half of all the known antibiotics. They are easy to isolate from soil but also form stable interactions with plant roots and can colonise the rhizosphere and endosphere of a wide range of different plants. We have found they are abundant inside wheat roots and that some strains can protect wheat against fungal diseases such as Take-all which can cause devastating crop losses. For one of these strains we have identified the molecules and genes responsible for this antifungal activity and shown it is increased two-fold when we add indole 3 acetic acid, a plant hormone present in wheat root exudates, to the growth medium. In this proposal we aim to look in more detail at the colonisation of wheat roots by soil dwelling, antibiotic-producing Streptomyces bacteria. We will sequence the genomes of 10 strains we have isolated from wheat plants which inhibit the take-all fungus on agar plates, identify the antifungals made by these strains and determine if they can protect wheat plants against take-all disease. We will also try to discover which molecules in wheat root exudates can feed these bacteria and switch on their production of secondary metabolites. Since Streptomyces bacteria form spores which can be dried and stored for long periods, we are interested in developing wheat seed coatings containing these spores so the bacteria grow into the roots of germinating wheat plants and protect them against disease. We will also test the role of the type VII secretion system in root colonisation by these strains since we have preliminary evidence that this system helps them outcompete other bacteria for wheat root colonisation. Our ultimate aim is to develop streptomycetes as seed coatings to protect wheat plants against fungal disease and to discover molecules from wheat root exudates that we can use to switch on secondary metabolite production in vitro because we know that 90% of the secondary metabolites they encode are not made under laboratory conditions. This could help us discover new and useful molecules from these bacteria.
植物利用光合作用将空气中的二氧化碳固定为它们用作食物的葡萄糖。然而,它们固定的高达40%的碳从它们的根部释放到土壤中,这似乎是一种从周围土壤中吸引有益细菌的机制。细菌利用根分泌物作为食物,这有助于植物组装健康的微生物群。其中一些微生物保护植物免受疾病侵袭,另一些微生物帮助植物从土壤中获得重要的养分。因此,根部微生物群对植物健康至关重要,但数百年来的作物育种选择了一些特征,如更大的植物具有更多的谷物,而可能忽略了对微生物群的(未知)影响。现在,人们越来越有兴趣操纵小麦等作物的微生物群,使它们更能抵抗疾病和干旱、盐碱等非生物胁迫。小麦是约40亿人的主要农作物,也是世界上最重要的作物之一,因此,如果我们要养活不断增长的世界人口,提高产量是至关重要的。我们的项目专注于一种名为链霉菌的土壤细菌属。已知的约600种链霉菌能产生大量的次生代谢产物,这些次生代谢产物占所有已知抗生素的一半。它们很容易从土壤中分离出来,但也能与植物根形成稳定的相互作用,并能在各种不同植物的根际和内圈定居。我们发现,它们在小麦根中含量丰富,一些菌株可以保护小麦免受全蚀病等真菌疾病的侵袭,这些疾病可能导致毁灭性的作物损失。对于其中一个菌株,我们已经确定了负责这种抗真菌活性的分子和基因,并表明当我们在生长介质中添加吲哚3乙酸时,这种抗真菌活性增加了两倍。吲哚3乙酸是一种存在于小麦根分泌物中的植物激素。在这项提案中,我们的目标是更详细地研究土壤中栖息的、产生抗生素的链霉菌对小麦根的定植。我们将对从小麦植株中分离出的10个抑制琼脂平板上全蚀病菌的菌株的基因组进行测序,鉴定这些菌株制造的抗真菌药物,并确定它们是否可以保护小麦植株免受全蚀病的侵染。我们还将试图发现小麦根分泌物中的哪些分子可以喂养这些细菌,并开启它们产生次生代谢物的过程。由于链霉菌形成的孢子可以干燥并长期储存,我们有兴趣开发包含这些孢子的小麦种衣剂,以便细菌生长到萌发的小麦植株的根中,并保护它们免受疾病的侵袭。我们还将测试VII型分泌系统在这些菌株根部定植中的作用,因为我们有初步证据表明,这个系统帮助它们在小麦根部定植方面胜过其他细菌。我们的最终目标是开发链霉菌作为种衣剂来保护小麦免受真菌疾病的侵染,并从小麦根分泌物中发现可以用来在体外启动次生代谢物产生的分子,因为我们知道它们编码的90%的次生代谢物不是在实验室条件下产生的。这可以帮助我们从这些细菌中发现新的有用的分子。

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Matthew Hutchings其他文献

A Simple Setup for Transfer Hydrogenations in Flow Chemistry
流动化学中转移氢化的简单设置
  • DOI:
    10.1055/s-0035-1561624
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    2
  • 作者:
    Matthew Hutchings;T. Wirth
  • 通讯作者:
    T. Wirth
Safe Use of Nitromethane for Aldol Reactions in Flow
硝基甲烷在流动中安全使用羟醛反应
  • DOI:
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    2.7
  • 作者:
    Matthew Hutchings;T. Wirth
  • 通讯作者:
    T. Wirth

Matthew Hutchings的其他文献

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{{ truncateString('Matthew Hutchings', 18)}}的其他基金

Manipulating two-component systems to activate cryptic antibiotic pathways in filamentous actinomycete bacteria
操纵双组分系统激活丝状放线菌中的神秘抗生素途径
  • 批准号:
    BB/Y005724/1
  • 财政年份:
    2024
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Post-translation regulation of antibiotic production in Streptomyces: the loaded gun hypothesis.
链霉菌抗生素生产的翻译后调控:装弹枪假说。
  • 批准号:
    BB/W000628/1
  • 财政年份:
    2022
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Regulation, biosynthesis and mode of action of formicamycins, promising new antibiotics with a high barrier to resistanc
福米霉素的调控、生物合成和作用方式,有望成为具有高耐药屏障的新型抗生素
  • 批准号:
    BB/S00811X/2
  • 财政年份:
    2020
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Regulation, biosynthesis and mode of action of formicamycins, promising new antibiotics with a high barrier to resistanc
福米霉素的调控、生物合成和作用方式,有望成为具有高耐药屏障的新型抗生素
  • 批准号:
    BB/S00811X/1
  • 财政年份:
    2019
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Understanding and manipulating a conserved and essential transcription factor to activate antibiotic production in Streptomyces species
了解和操纵保守且必需的转录因子以激活链霉菌物种中的抗生素生产
  • 批准号:
    BB/P005292/1
  • 财政年份:
    2017
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Partner choice: How does a host select and control its microbiome?
合作伙伴选择:宿主如何选择和控制其微生物组?
  • 批准号:
    NE/M015033/1
  • 财政年份:
    2015
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Let the right ones in: Testing microeconomic models of screening in an ant-bacteria microbiome
让合适的人进来:测试抗菌微生物组筛选的微观经济模型
  • 批准号:
    NE/J01074X/1
  • 财政年份:
    2012
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Isolation and characterisation of novel antimycotics
新型抗真菌药的分离和表征
  • 批准号:
    G0801721/1
  • 财政年份:
    2009
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Processing of cell surface lipoproteins in Streptomyces coelicolor. A new paradigm?
天蓝色链霉菌细胞表面脂蛋白的加工。
  • 批准号:
    BB/F009429/1
  • 财政年份:
    2007
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant

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Segmented Filamentous Bacteria激活宿主免疫系统抑制其拮抗菌 Enterobacteriaceae维持菌群平衡及其机制研究
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水稻种子际固有细菌的群落多样性及其瞬时演替研究
  • 批准号:
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    2003
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相似海外基金

Streptomyces bacteria: antibiotic production in the wheat endosphere
链霉菌:小麦内圈产生抗生素
  • 批准号:
    BB/T015632/1
  • 财政年份:
    2021
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Grant
Control of antibiotic production and development in Streptomyces bacteria
链霉菌抗生素生产和发育的控制
  • 批准号:
    552591-2020
  • 财政年份:
    2020
  • 资助金额:
    $ 64.04万
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    University Undergraduate Student Research Awards
Exploring new avenues of growth and secondary metabolite production by antibiotic-producing Streptomyces bacteria
探索产抗生素链霉菌生长和次生代谢产物生产的新途径
  • 批准号:
    418836285
  • 财政年份:
    2019
  • 资助金额:
    $ 64.04万
  • 项目类别:
    Research Fellowships
Overcoming barriers to antibiotic production in Streptomyces bacteria
克服链霉菌细菌生产抗生素的障碍
  • 批准号:
    390484
  • 财政年份:
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  • 项目类别:
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Developing new tools for antibiotic discovery in Streptomyces bacteria (HUTCHINGS_U17DTP)
开发用于链霉菌抗生素发现的新工具 (HUTCHINGS_U17DTP)
  • 批准号:
    1937459
  • 财政年份:
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The regulation of development in the antibiotic-producing bacteria Streptomyces (BUTTNER_J17DTP)
抗生素生产菌链霉菌 (BUTTNER_J17DTP) 的发育调控
  • 批准号:
    1911023
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Cyclic di-GMP in the control of multicellular differentiation in antibiotic-producing bacteria Streptomyces.
环状二-GMP 控制抗生素生产细菌链霉菌的多细胞分化。
  • 批准号:
    282982160
  • 财政年份:
    2016
  • 资助金额:
    $ 64.04万
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Investigating how plants recruit antibiotic-producing Streptomyces bacteria to protect themselves against disease
研究植物如何招募产生抗生素的链霉菌来保护自己免受疾病侵害
  • 批准号:
    1799823
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Relationship of Antibiotic Production and Development in Streptomyces coelicolor
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  • 批准号:
    7939390
  • 财政年份:
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Regulation of Streptomyces differentiation and antibiotic production by SoxR
SoxR 对链霉菌分化和抗生素生产的调节
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