The RGG/RG motif as an RNA chaperone: advancing CRISPR-Cas RNA technology

RGG/RG 基序作为 RNA 伴侣：推进 CRISPR-Cas RNA 技术

• 批准号: DP200102737
• 负责人: Prof Jacqueline Wilce
• 金额: $ 29.49万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2020
• 资助国家: 澳大利亚
• 起止时间: 2020-01-01 至 2023-11-22
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP200102737
• 关键词: RGG; RG; motif; RNA; chaperone

This project investigates the way in which protein molecules interact effectively with RNA molecules and also aims to enhance the CRISPR-Cas13a system for RNA detection. Innovative approaches will be used to test the role of a particular protein motif, called the RGG/RG motif, in remodelling RNA structure and enhancing the Cas13a protein. This knowledge is expected to shift our understanding of protein-RNA interactions that are fundamental to almost every aspect of cell biology. The project is intended to benefit Australia through contributing to fundamental knowledge in the field, facilitating the development of new CRISPR-Cas biotechnologies for RNA detection and through the training of young researchers in frontier technologies.

该项目研究了蛋白质分子与RNA分子有效相互作用的方式，并旨在增强CRISPR-Cas 13 a系统的RNA检测能力。创新的方法将被用来测试一个特定的蛋白质基序，称为RGG/RG基序，在重塑RNA结构和增强Cas 13 a蛋白质的作用。这一知识有望改变我们对蛋白质-RNA相互作用的理解，而蛋白质-RNA相互作用几乎是细胞生物学各个方面的基础。该项目旨在通过促进该领域的基础知识，促进用于RNA检测的新CRISPR-Cas生物技术的开发，以及通过培训前沿技术的年轻研究人员，使澳大利亚受益。