Functional dissection of pectic rhamnogalacturonan-I (RG-I) in plant cell walls

植物细胞壁果胶鼠李半乳糖醛酸-I (RG-I) 的功能解剖

基本信息

  • 批准号:
    BB/K017489/1
  • 负责人:
  • 金额:
    $ 52.85万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2014
  • 资助国家:
    英国
  • 起止时间:
    2014 至 无数据
  • 项目状态:
    已结题

项目摘要

Plant cell walls are rigid structures at the surface of all plant cells and are responsible for generating the mechanical properties of growing plants and many products/materials derived from crops such as fruits, vegetables and textile fibres. Cell walls are mechanically strong biomaterials and are highly complex in terms of structure. They are comprised of varied configurations of a range of polysaccharides that include cellulose, hemicelluloses and pectic polysaccharides. The pectic components of plants cell walls are particularly structurally highly complex and are entwined within cell walls with the cellulose and other polysaccharides. The roles of pectin in cell wall assembly and the generation of cell wall properties leading to the properties of plant materials are not understood. A portion of the pectic polysaccharides are a structurally complex and highly variable set of polysaccharides that are known as rhamnogalacturonan-I (RGI) due to the presence of a structural backbone that contains the sugars rhamnose and galacturonic acid. This polysaccharide backbone has side chains rich in arabinose and galactose sugars. The individual structures of RG-I domains appear to vary from cell to cell and several lines of evidence suggest that they have a role in generating cell wall properties influencing factors such as the extensibility and firmness of cells and tissues. The project will develop a new set of tools to identify and track individual RGI molecules using the model plant system Arabidopsis. This work in generating understanding of the profiles of RGI components at a microscale level in a single leaf or root will open up a new area for plant biology with the prospect of controlling the mechanical and textural properties of plant materials. The project will use the analytic methods developed in the first part of the programme to study RGI structure and functions in the context of plant growth. Growing plants have to withstand a range of mechanical stresses - whether this is wind and rain that constantly stress shoots and leaves or roots growing through soil with varying degrees of compactness. Little is known about how cell walls and plant organs respond to these mechanical stresses although preliminary evidence indicates that RGI molecules are involved generating the mechanics of cells and organs and also involved in responses to stress. The tools and approaches for RGI analysis developed in the project will be used to study the variation and modification of RGI molecules subject to mechanical stresses during growth. An understanding of how RGI impacts on cell wall properties such as firmness and elasticity to cell walls will generate important knowledge to understand how cell wall properties are generated. The work will impact on our understanding and capacity for exploitation of a range of plant materials and products from texture of eaten products such as apple and tomato fruits to wheat grain to cotton fibres. It will also provide underpinning understanding of how crop plants respond to mechanical stress imposed by climatic factors and environmental stresses.
植物细胞壁是所有植物细胞表面的刚性结构,负责产生生长中的植物和许多来自水果、蔬菜和纺织纤维等作物的产品/材料的机械性能。细胞壁是一种机械强度很高的生物材料,结构非常复杂。它们由不同构型的多糖组成,包括纤维素、半纤维素和果胶多糖。植物细胞壁的果胶成分在结构上特别复杂,与纤维素和其他多糖在细胞壁中缠绕在一起。果胶在细胞壁组装中的作用以及导致植物材料特性的细胞壁特性的产生还不清楚。果胶多糖的一部分是一组结构复杂和高度可变的多糖组,被称为鼠李半乳糖苷-I(RGI),这是因为存在包含糖、鼠李糖和半乳糖醛酸的结构主干。这种多糖主链含有富含阿拉伯糖和半乳糖的侧链。RG-I结构域的个体结构似乎在不同的细胞之间有所不同,一些证据表明,它们在细胞壁属性的产生中起到作用,影响因素如细胞和组织的延展性和坚固性。该项目将开发一套新的工具,使用拟南芥模式植物系统来识别和跟踪单个RGI分子。这项工作在了解RGI组分在单个叶或根中的微观水平上的分布,将为植物生物学开辟一个新的领域,并有望控制植物材料的机械和质地特性。该项目将使用方案第一部分开发的分析方法,在植物生长的背景下研究RGI的结构和功能。生长中的植物必须承受一系列机械压力--无论是不断给枝叶施加压力的风和雨,还是通过不同紧实度的土壤生长的根。尽管初步证据表明RGI分子参与了细胞和器官的机械反应,也参与了对胁迫的反应,但人们对细胞壁和植物器官如何响应这些机械压力知之甚少。该项目开发的RGI分析工具和方法将用于研究RGI分子在生长过程中受到机械应力的变化和修饰。了解RGI如何影响细胞壁属性,如对细胞壁的坚固性和弹性,将产生重要的知识,以理解细胞壁属性是如何产生的。这项工作将影响我们对一系列植物材料和产品的理解和开发能力,从苹果和西红柿水果等食用产品的质地到小麦谷物再到棉纤维。它还将提供对作物如何响应气候因素和环境压力施加的机械压力的基础理解。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Xyloglucan is released by plants and promotes soil particle aggregation.
  • DOI:
    10.1111/nph.14897
  • 发表时间:
    2018-03
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Galloway AF;Pedersen MJ;Merry B;Marcus SE;Blacker J;Benning LG;Field KJ;Knox JP
  • 通讯作者:
    Knox JP
A soil-binding polysaccharide complex released from root hairs functions in rhizosheath formation
从根毛释放的土壤结合多糖复合物在根鞘形成中发挥作用
  • DOI:
    10.1101/2021.04.15.440065
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Galloway A
  • 通讯作者:
    Galloway A
Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: Evidence for sub-populations in fruit parenchyma systems.
  • DOI:
    10.1016/j.foodchem.2017.11.025
  • 发表时间:
    2018-04-25
  • 期刊:
  • 影响因子:
    8.8
  • 作者:
    Cornuault V;Posé S;Knox JP
  • 通讯作者:
    Knox JP
Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls.
  • DOI:
    10.1007/s00425-015-2375-4
  • 发表时间:
    2015-12
  • 期刊:
  • 影响因子:
    4.3
  • 作者:
    Cornuault V;Buffetto F;Rydahl MG;Marcus SE;Torode TA;Xue J;Crépeau MJ;Faria-Blanc N;Willats WG;Dupree P;Ralet MC;Knox JP
  • 通讯作者:
    Knox JP
Altered properties and structures of root exudate polysaccharides in a root hairless mutant of barley.
  • DOI:
    10.1093/plphys/kiac341
  • 发表时间:
    2022-09-28
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    Galloway, Andrew F.;Akhtar, Jumana;Burak, Emma;Marcus, Susan E.;Field, Katie J.;Dodd, Ian C.;Knox, Paul
  • 通讯作者:
    Knox, Paul
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Paul Knox其他文献

Rebreathing improves accuracy of ventilatory monitoring
The passage of Bill 39 : reform and repression in British Columbia’s labour policy
  • DOI:
    10.14288/1.0099887
  • 发表时间:
    1974
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Paul Knox
  • 通讯作者:
    Paul Knox
The role of cell wall-based defences in the early restriction of non-pathogenic <em>hrp</em> mutant bacteria in Arabidopsis
  • DOI:
    10.1016/j.phytochem.2014.07.015
  • 发表时间:
    2015-04-01
  • 期刊:
  • 影响因子:
  • 作者:
    Kathy Mitchell;Ian Brown;Paul Knox;John Mansfield
  • 通讯作者:
    John Mansfield
Insecticidal activity of hypericin towardsManduca sexta larvae
  • DOI:
    10.1007/bf01015181
  • 发表时间:
    1989-03-01
  • 期刊:
  • 影响因子:
    1.800
  • 作者:
    Richard Samuels;Paul Knox
  • 通讯作者:
    Paul Knox

Paul Knox的其他文献

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{{ truncateString('Paul Knox', 18)}}的其他基金

The virtual seed (vSEED)
虚拟种子 (vSEED)
  • 批准号:
    BB/G024898/1
  • 财政年份:
    2009
  • 资助金额:
    $ 52.85万
  • 项目类别:
    Research Grant
Dissecting the role of carbohydrate binding modules in plant cell wall degradation
剖析碳水化合物结合模块在植物细胞壁降解中的作用
  • 批准号:
    BB/E014364/1
  • 财政年份:
    2007
  • 资助金额:
    $ 52.85万
  • 项目类别:
    Research Grant
Cell wall microstructure and plant cell separation
细胞壁微观结构与植物细胞分离
  • 批准号:
    BB/D00098X/1
  • 财政年份:
    2006
  • 资助金额:
    $ 52.85万
  • 项目类别:
    Research Grant
The Structures of and Processes of Building Provision: A Case Study of Master-Planned Communities
建筑供给的结构和过程:总体规划社区的案例研究
  • 批准号:
    9114399
  • 财政年份:
    1991
  • 资助金额:
    $ 52.85万
  • 项目类别:
    Standard Grant

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