EARLY WEANING AND GUT DEVELOPMENT-ROLE OF ODC AND TYR-K

早期断奶和肠道发育 - ODC 和 TYR-K 的作用

• 批准号: 2133948
• 负责人: CHUAN-HAO LIN
• 金额: $ 8.72万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2133948
• 关键词: amylases; enzyme activity; enzyme inhibitors; enzyme mechanism; gene expression; histogenesis; in situ hybridization; infant animal; laboratory rat; lipase; northern blottings; nuclear runoff assay; nutrient intake activity; ornithine decarboxylase; pancreas; polymerase chain reaction; protein tyrosine kinase; small intestines

Our preliminary studies in early weaned (15 d/o) neonatal rats showed the induction of proximal small intestinal and pancreatic ornithine decarboxylase (ODC) activities. The induction of ODC was 1-2 day earlier than the precocious expression of proximal showed for the first time the ontogeny of intestinal tyrosine kinases (Tyr-k) with parallelism between Tyr-k and growth and maturation of the rat small intestine. The overall aim of the current Grant Proposal is to define, at the cellular and gene expression level, the effect of early weaning on small intestinal and pancreatic development in neonatal rats. We will examine the role of these two proliferative enzymes-ODC and Tyr-K in the development of small intestine and pancreas after early weaning. Specifically we will: 1) Evaluate the growth and development parameters of small intestine and pancreas after early weaning and its relationship with ODC and Tyr-k. 2) Examine the essential roles of ODC and Tyr-K in the development of small intestine and pancreas after early weaning by administration of its respective inhibitor and assess the resultant effects of the development of these two organs. 3) Study (i) gene expression of ODC, Tyr-k (EGF-R Tyr-k and pp60c-src Tyr-k of small intestine, after early weaning. The findings from current proposed studies will enable us to understand the intracellular events and its regulatory mechanisms of developmental process of small intestine and pancreas in weaning and early weaning.

我们对早期断奶（15 d/o）新生大鼠的初步研究表明， 近端小肠和胰腺鸟氨酸诱导 脱羧酶（ODC）活性。 ODC诱导提前1-2天 比近端的早熟表达首次显示， 肠酪氨酸激酶（Tyr-k）的个体发生与 酪氨酸激酶与大鼠小肠的生长和成熟。 整体 目前的拨款提案的目的是定义，在细胞和基因 表达水平，早期断奶对小肠和 新生大鼠胰腺发育。 我们将研究 这两种增殖酶--ODC和Tyr-K在小 肠和胰腺的生长。 具体而言，我们将：1） 评价小肠生长发育参数， 早期断乳后胰腺组织中ODC和Tyr-k的变化及其与ODC和Tyr-k的关系。（二） 研究ODC和Tyr-K在小分子药物开发中的重要作用 肠和胰腺后，早期断奶，通过其管理 相应的抑制剂，并评估开发的结果影响 这两个器官。3)研究（i）ODC，Tyr-k（EGF-R）的基因表达 早期断奶后小肠的Tyr-k和pp 60 c-src Tyr-k。 的 目前拟议研究的结果将使我们能够了解 发育的细胞内事件及其调控机制 断奶和早期断奶时小肠和胰腺的过程。