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EARLY WEANING AND GUT DEVELOPMENT-ROLE OF ODC AND TYR-K

早期断奶和肠道发育 - ODC 和 TYR-K 的作用

基本信息

批准号：
2749391
负责人：
CHUAN-HAO LIN
金额：
$ 10.49万
依托单位：
WAYNE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-02-01 至 2002-07-31
项目状态：
已结题

项目摘要

Our preliminary studies in early weaned (15 d/o) neonatal rats showed the induction of proximal small intestinal and pancreatic ornithine decarboxylase (ODC) activities. The induction of ODC was 1-2 day earlier than the precocious expression of proximal showed for the first time the ontogeny of intestinal tyrosine kinases (Tyr-k) with parallelism between Tyr-k and growth and maturation of the rat small intestine. The overall aim of the current Grant Proposal is to define, at the cellular and gene expression level, the effect of early weaning on small intestinal and pancreatic development in neonatal rats. We will examine the role of these two proliferative enzymes-ODC and Tyr-K in the development of small intestine and pancreas after early weaning. Specifically we will: 1) Evaluate the growth and development parameters of small intestine and pancreas after early weaning and its relationship with ODC and Tyr-k. 2) Examine the essential roles of ODC and Tyr-K in the development of small intestine and pancreas after early weaning by administration of its respective inhibitor and assess the resultant effects of the development of these two organs. 3) Study (i) gene expression of ODC, Tyr-k (EGF-R Tyr-k and pp60c-src Tyr-k of small intestine, after early weaning. The findings from current proposed studies will enable us to understand the intracellular events and its regulatory mechanisms of developmental process of small intestine and pancreas in weaning and early weaning.

我们对早期断奶(15d/o)新生大鼠的初步研究表明近端小肠和胰腺鸟氨酸的诱导脱羧酶(ODC)活性。ODC的诱导时间提前1-2天近端的早熟表达首次显示出肠酪氨酸激酶(Tyr-k)的个体发育及其与酪氨酸酶的平行 Tyr-k与大鼠小肠生长成熟的关系。整体而言目前的赠款提案的目的是在细胞和基因上定义表达水平、早期断奶对小肠和新生大鼠胰腺的发育。我们将研究这一角色这两种增殖酶--ODC和Tyr-K在小鼠发育中的作用早期断奶后的肠道和胰腺。具体来说，我们将：1) 测定小鼠小肠的生长发育参数早期断奶后胰腺及其与ODC和Tyr-k的关系2) 探讨ODC和Tyr-K在Small发育中的重要作用 ITS对早期断奶后大鼠肠道和胰腺的影响分别抑制和评估发展的综合影响这两个器官。3)研究(I)ODC、Tyr-k(EGF-R)基因表达早期断奶后小肠组织中的Tyr-k和pp60c-src Tyr-k。这个目前拟议的研究结果将使我们能够了解发育相关的细胞内事件及其调控机制断奶和早期断奶过程中小肠和胰腺的变化。