KIDNEY CA++ PUMP OF PLASMA MEMBRANES

肾 CA 质膜泵

• 批准号: 2144166
• 负责人: JOHN T PENNISTON
• 金额: $ 19.01万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2144166
• 关键词: adenosinetriphosphatase; biological signal transduction; calcium flux; cell membrane; erythrocytes; genetic library; isozymes; laboratory rabbit; laboratory rat; molecular cloning; monoclonal antibody; polymerase chain reaction; protein structure; renal tubule; synthetic peptide

The overall aim of this project is to investigate the role of the plasma membrane Ca2+ pump in Ca2+ transport in kidney, particularly the kidneys of rat. Both monoclonal antibodies and gene cloning techniques (polymerase chain reaction) will be used to explore the expression of different isozymes along the tubule. It is likely that the Ca2+ pump has multiple roles in the nephron, including its involvement in control of intracellular Ca2+ signalling and its role in transcellular transport of Ca2+ (by means of which the kidney recaptures Ca2+ from the urine). Different isozymes of the Ca2+ pump are likely to be involved in different roles, and probably differ from one another in properties such as calcium affinity, regulation by protein kinases and sensitivity to calmodulin. Determination of the distribution of Ca2+ pump isoforms along the nephron will provide fundamental knowledge needed to understand renal handling of calcium in health and disease.

这个项目的总体目标是研究等离子体的作用 膜Ca 2+泵在肾脏Ca 2+转运中的作用 大鼠 单克隆抗体和基因克隆技术（聚合酶 链反应）将被用来探索不同的表达 沿着小管。 Ca 2+泵可能具有多个 在肾单位中的作用，包括其参与控制细胞内 Ca ~（2+）信号转导及其在Ca ~（2+）跨细胞转运中的作用（方法 其中肾脏从尿中重新捕获Ca 2+）。 不同的同工酶 Ca 2+泵可能参与不同作用， 在特性上彼此不同，例如钙亲和力、调节 和对钙调蛋白的敏感性。 测定 Ca 2+泵亚型沿着肾单位的分布将提供 了解肾脏对钙的处理所需的基础知识， 健康和疾病。