KIDNEY CA++ PUMP OF PLASMA MEMBRANES

该项目的总体目标是研究等离子体的作用 肾脏中 Ca2 运输中的膜 Ca2 泵，特别是肾脏 老鼠。 单克隆抗体和基因克隆技术（聚合酶 链式反应）将被用来探索不同的表达 沿着肾小管的同工酶。 Ca2 泵可能有多个 在肾单位中的作用，包括其参与细胞内的控制 Ca2 信号传导及其在 Ca2 跨细胞转运中的作用（通过手段 其中肾脏从尿液中重新捕获 Ca2）。 不同的同工酶 Ca2 泵可能发挥不同的作用，并且可能 钙亲和力、调节性等特性各不相同 通过蛋白激酶和对钙调蛋白的敏感性。 测定 Ca2 泵亚型沿肾单位的分布将提供 了解肾脏对钙的处理所需的基础知识 健康和疾病。

The overall aim of this project is to investigate the role of the plasma membrane Ca2+ pump in Ca2+ transport in kidney, particularly the kidneys of rat. Both monoclonal antibodies and gene cloning techniques (polymerase chain reaction) will be used to explore the expression of different isozymes along the tubule. It is likely that the Ca2+ pump has multiple roles in the nephron, including its involvement in control of intracellular Ca2+ signalling and its role in transcellular transport of Ca2+ (by means of which the kidney recaptures Ca2+ from the urine). Different isozymes of the Ca2+ pump are likely to be involved in different roles, and probably differ from one another in properties such as calcium affinity, regulation by protein kinases and sensitivity to calmodulin. Determination of the distribution of Ca2+ pump isoforms along the nephron will provide fundamental knowledge needed to understand renal handling of calcium in health and disease.