KIDNEY CA++ PUMP OF PLASMA MEMBRANES

这个项目的总体目标是研究血浆的作用 膜钙泵在肾脏，尤其是肾脏中的钙转运 老鼠。单抗和基因克隆技术(聚合酶 连锁反应)将被用来探索不同的表达 沿小管的同工酶。很可能钙离子泵有多个 肾单位的作用，包括参与细胞内的控制 CA2信号及其在钙跨细胞运输中的作用(通过 其中肾脏从尿液中重新捕获钙)。黄瓜不同同工酶 钙泵可能参与不同的角色，并可能 在钙亲和力、调节性、 由蛋白激酶和对钙调蛋白的敏感性决定。确定了 钙泵异构体沿肾单位的分布将提供 了解肾脏对钙的处理所需的基础知识 健康和疾病。

The overall aim of this project is to investigate the role of the plasma membrane Ca2+ pump in Ca2+ transport in kidney, particularly the kidneys of rat. Both monoclonal antibodies and gene cloning techniques (polymerase chain reaction) will be used to explore the expression of different isozymes along the tubule. It is likely that the Ca2+ pump has multiple roles in the nephron, including its involvement in control of intracellular Ca2+ signalling and its role in transcellular transport of Ca2+ (by means of which the kidney recaptures Ca2+ from the urine). Different isozymes of the Ca2+ pump are likely to be involved in different roles, and probably differ from one another in properties such as calcium affinity, regulation by protein kinases and sensitivity to calmodulin. Determination of the distribution of Ca2+ pump isoforms along the nephron will provide fundamental knowledge needed to understand renal handling of calcium in health and disease.