Properties of Calcium Pumping ATPase

钙泵 ATP 酶的特性

基本信息

  • 批准号:
    6541634
  • 负责人:
  • 金额:
    $ 31.46万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1993
  • 资助国家:
    美国
  • 起止时间:
    1993-07-01 至 2006-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Studies on the properties of the calcium ion pumping ATPase of plasma membranes will be continued. These calcium ion pumps exist in the plasma membranes of heart, smooth muscle, kidney, brain, intestinal epithelium, erythrocyte and many other cell types. They are involved in control of intracellular free calcium levels in cells of all kinds, and in movement of calcium ions across cell layers such as the intestinal and kidney tubule epithelia. Information about the regulation of these pumps may thus give information relevant to hypertension and kidney disease, among other conditions. The pump consists of a single polypeptide chain of molecular weight 127,000-137,000 (the exact molecular weight depends on the isoform). Four genes encode different isoforms, and additional forms are created by alternate splices. We have succeeded in expressing this pump in COS and insect cells and have developed assay systems for determining the activity of the expressed enzyme, its binding of calmodulin and of calcium ions. We propose studies designed to extend our knowledge of the function of the different isoforms of this enzyme, and of the calmodulin-binding and calcium ion-binding residues. We will make measurements of the nitrophenyl phosphatase activity, of the rates of the activation of the pump and of the binding of calmodulin. Measurements will also be made to observe the memory of the pump for its previous history of calcium ion exposure. These measurements will be made on appropriately altered forms of the pump and on the different isoforms.
描述(由申请人提供):将继续研究质膜钙离子泵ATP酶的性质。这些钙离子泵存在于心脏、平滑肌、肾、脑、肠上皮、红细胞和许多其他细胞类型的质膜中。它们参与控制各种细胞中的细胞内游离钙水平,并参与钙离子穿过细胞层如肠和肾小管上皮细胞的运动。因此,关于这些泵的调节的信息可以提供与高血压和肾脏疾病以及其他疾病相关的信息。 该泵由分子量为127,000 - 137,000的单一多肽链组成(确切分子量取决于同种型)。四个基因编码不同的同种型,另外的形式是由交替剪接产生的。我们已经成功地在COS和昆虫细胞中表达了这种泵,并开发了用于确定所表达的酶的活性、其与钙调素和钙离子的结合的测定系统。我们提出的研究旨在扩大我们的知识,这种酶的不同亚型的功能,钙调素结合和钙离子结合残基。我们将测量硝基苯磷酸酶活性、泵的激活速率和钙调蛋白的结合速率。还将进行测量,以观察胰岛素泵对先前钙离子暴露历史的记忆。这些测量将在适当改变的泵形式和不同的同种型上进行。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

JOHN T PENNISTON其他文献

JOHN T PENNISTON的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('JOHN T PENNISTON', 18)}}的其他基金

ROLE OF THE PLASMA MEMBRANE CALCIUM PUMP IN HEARING
等离子膜钙泵在听力中的作用
  • 批准号:
    6523489
  • 财政年份:
    2000
  • 资助金额:
    $ 31.46万
  • 项目类别:
ROLE OF THE PLASMA MEMBRANE CALCIUM PUMP IN HEARING
等离子膜钙泵在听力中的作用
  • 批准号:
    6379511
  • 财政年份:
    2000
  • 资助金额:
    $ 31.46万
  • 项目类别:
ROLE OF THE PLASMA MEMBRANE CALCIUM PUMP IN HEARING
等离子膜钙泵在听力中的作用
  • 批准号:
    6189518
  • 财政年份:
    2000
  • 资助金额:
    $ 31.46万
  • 项目类别:
REGULATION OF CA PUMP BY THE PROTEIN KINASE PATHWAY
蛋白激酶途径对 CA 泵的调节
  • 批准号:
    2024072
  • 财政年份:
    1997
  • 资助金额:
    $ 31.46万
  • 项目类别:
REGULATION OF CA PUMP BY THE PROTEIN KINASE PATHWAY
蛋白激酶途径对 CA 泵的调节
  • 批准号:
    6019263
  • 财政年份:
    1997
  • 资助金额:
    $ 31.46万
  • 项目类别:
REGULATION OF CA PUMP BY THE PROTEIN KINASE PATHWAY
蛋白激酶途径对 CA 泵的调节
  • 批准号:
    2750139
  • 财政年份:
    1997
  • 资助金额:
    $ 31.46万
  • 项目类别:
REGULATION OF CA PUMP BY THE PROTEIN KINASE PATHWAY
蛋白激酶途径对 CA 泵的调节
  • 批准号:
    6180679
  • 财政年份:
    1997
  • 资助金额:
    $ 31.46万
  • 项目类别:
KIDNEY CA++ PUMP OF PLASMA MEMBRANES
肾 CA 质膜泵
  • 批准号:
    660345
  • 财政年份:
    1995
  • 资助金额:
    $ 31.46万
  • 项目类别:
KIDNEY CA++ PUMP OF PLASMA MEMBRANES
肾 CA 质膜泵
  • 批准号:
    2144165
  • 财政年份:
    1994
  • 资助金额:
    $ 31.46万
  • 项目类别:
KIDNEY CA++ PUMP OF PLASMA MEMBRANES
肾 CA 质膜泵
  • 批准号:
    2144168
  • 财政年份:
    1992
  • 资助金额:
    $ 31.46万
  • 项目类别:

相似海外基金

Bridging the Gap: Next-Gen Tools for Accurate Prediction of Disordered Protein Binding Sites
弥合差距:准确预测无序蛋白质结合位点的下一代工具
  • 批准号:
    24K15172
  • 财政年份:
    2024
  • 资助金额:
    $ 31.46万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Design of protein crystal templates with multiple binding sites for tracking metal complex reactions.
设计具有多个结合位点的蛋白质晶体模板,用于跟踪金属络合物反应。
  • 批准号:
    23K04928
  • 财政年份:
    2023
  • 资助金额:
    $ 31.46万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Dynamic changes in PIP2 binding sites and their impact on axonal targeting and function of epilepsy-associated KCNQ/Kv7 channels
PIP2 结合位点的动态变化及其对癫痫相关 KCNQ/Kv7 通道的轴突靶向和功能的影响
  • 批准号:
    10744934
  • 财政年份:
    2023
  • 资助金额:
    $ 31.46万
  • 项目类别:
Computational methods to identify small molecule RNA binding sites
识别小分子 RNA 结合位点的计算方法
  • 批准号:
    573688-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 31.46万
  • 项目类别:
    University Undergraduate Student Research Awards
Identification of potential drug binding sites within allosteric networks in cyclic nucleotide modulated channels
环核苷酸调节通道变构网络内潜在药物结合位点的鉴定
  • 批准号:
    10704557
  • 财政年份:
    2022
  • 资助金额:
    $ 31.46万
  • 项目类别:
Identification of potential drug binding sites within allosteric networks in cyclic nucleotide modulated channels
环核苷酸调节通道变构网络内潜在药物结合位点的鉴定
  • 批准号:
    10537846
  • 财政年份:
    2022
  • 资助金额:
    $ 31.46万
  • 项目类别:
Identifying new types of inhibitors in quinone binding sites in photosynthetic enzymes
鉴定光合酶醌结合位点的新型抑制剂
  • 批准号:
    2753921
  • 财政年份:
    2022
  • 资助金额:
    $ 31.46万
  • 项目类别:
    Studentship
Development of broad nanovaccines targeting diverse coronavirus receptor-binding sites
开发针对不同冠状病毒受体结合位点的广泛纳米疫苗
  • 批准号:
    10328140
  • 财政年份:
    2022
  • 资助金额:
    $ 31.46万
  • 项目类别:
Exploiting Water Network Perturbations in Protein Binding Sites
利用蛋白质结合位点的水网络扰动
  • 批准号:
    10621368
  • 财政年份:
    2021
  • 资助金额:
    $ 31.46万
  • 项目类别:
SBIR Phase I: Nonlinear optical method for identifying protein-ligand binding sites
SBIR 第一阶段:识别蛋白质-配体结合位点的非线性光学方法
  • 批准号:
    2111821
  • 财政年份:
    2021
  • 资助金额:
    $ 31.46万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了