REGULATION OF CA PUMP BY THE PROTEIN KINASE PATHWAY

蛋白激酶途径对 CA 泵的调节

基本信息

  • 批准号:
    2024072
  • 负责人:
  • 金额:
    $ 6.18万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1997
  • 资助国家:
    美国
  • 起止时间:
    1997-08-01 至 2001-07-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION: This is a study of the protein kinase pathway by which extracellular agonists regulate the plasma membrane Ca2+ pump. The Ca2+ pump exists in the plasma membrane of heart, smooth muscle, kidney, brain, intestinal epithelium, erythrocyte and many other cell types. It is involved in control of intracellular Ca2+ levels in cells of all kinds and in movement of Ca2+ across epithelial cell layers such as the intestinal and kidney tubule epithelia. Studies on the phosphorylation of plasma membrane Ca2+ pump by protein kinases will be combined with studies on the effect of such phosphorylation on the activity of the plasma membrane Ca2+ pump. Information about the effect of protein kinases on this pump will provide an essential link in determining how agonists influence intracellular function by means of the Ca2+ signal. The pump consists of a single polypeptide chain of molecular weight about 135,000 (the exact molecular weight depends on the isoform). We have succeeded in expressing this pump in COS cells and have developed an assay system for determining activity of the expressed enzyme. We propose studies designed to determine the exact locus and nature of the control of the pump s activities by protein kinases. We will use truncated mutants and point mutants of the pump to determine the exact site of phosphorylation and will carry out activity assays under conditions almost identical to those of phosphorylation to determine how the phosphorylation at a given point activates the enzyme. We will raise antibodies against the phosphoenzymes, which should be very useful in determining the role of phosphorylation after stimulation of specific kinds of cells.
描述:这是一项蛋白激酶途径的研究

项目成果

期刊论文数量(0)
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专利数量(0)

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JOHN T PENNISTON其他文献

JOHN T PENNISTON的其他文献

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{{ truncateString('JOHN T PENNISTON', 18)}}的其他基金

ROLE OF THE PLASMA MEMBRANE CALCIUM PUMP IN HEARING
等离子膜钙泵在听力中的作用
  • 批准号:
    6523489
  • 财政年份:
    2000
  • 资助金额:
    $ 6.18万
  • 项目类别:
ROLE OF THE PLASMA MEMBRANE CALCIUM PUMP IN HEARING
等离子膜钙泵在听力中的作用
  • 批准号:
    6379511
  • 财政年份:
    2000
  • 资助金额:
    $ 6.18万
  • 项目类别:
ROLE OF THE PLASMA MEMBRANE CALCIUM PUMP IN HEARING
等离子膜钙泵在听力中的作用
  • 批准号:
    6189518
  • 财政年份:
    2000
  • 资助金额:
    $ 6.18万
  • 项目类别:
REGULATION OF CA PUMP BY THE PROTEIN KINASE PATHWAY
蛋白激酶途径对 CA 泵的调节
  • 批准号:
    6019263
  • 财政年份:
    1997
  • 资助金额:
    $ 6.18万
  • 项目类别:
REGULATION OF CA PUMP BY THE PROTEIN KINASE PATHWAY
蛋白激酶途径对 CA 泵的调节
  • 批准号:
    2750139
  • 财政年份:
    1997
  • 资助金额:
    $ 6.18万
  • 项目类别:
REGULATION OF CA PUMP BY THE PROTEIN KINASE PATHWAY
蛋白激酶途径对 CA 泵的调节
  • 批准号:
    6180679
  • 财政年份:
    1997
  • 资助金额:
    $ 6.18万
  • 项目类别:
KIDNEY CA++ PUMP OF PLASMA MEMBRANES
肾 CA 质膜泵
  • 批准号:
    660345
  • 财政年份:
    1995
  • 资助金额:
    $ 6.18万
  • 项目类别:
KIDNEY CA++ PUMP OF PLASMA MEMBRANES
肾 CA 质膜泵
  • 批准号:
    2144165
  • 财政年份:
    1994
  • 资助金额:
    $ 6.18万
  • 项目类别:
Properties of Calcium Pumping ATPase
钙泵 ATP 酶的特性
  • 批准号:
    6541634
  • 财政年份:
    1993
  • 资助金额:
    $ 6.18万
  • 项目类别:
KIDNEY CA++ PUMP OF PLASMA MEMBRANES
肾 CA 质膜泵
  • 批准号:
    2144166
  • 财政年份:
    1992
  • 资助金额:
    $ 6.18万
  • 项目类别:

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