U1 SNRNA GENE EXPRESSION AND TRANSCRIPTION

U1 SNRNA 基因表达和转录

• 批准号: 3734266
• 负责人: GRACIELA C CANDELAS
• 金额: --
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734266
• 关键词: Arachnida; RNA biosynthesis; cell free system; computer assisted sequence analysis; gene expression; genetic regulation; genetic transcription; messenger RNA; molecular cloning; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; protein biosynthesis; restriction mapping; secretory protein; small nuclear RNA

The long range aim of the project is to analyze the synthesis of a tissue- specific product using a suitable and fruitful model system. The immediate aim of this proposal is to investigate the timely tissue-specific accumulation of U1 small nuclear RNA (U1 snRNA) elicited by the stimulation into protein synthesis of fibroin producing glands (the model system). U1 snRNA plays a vital role in protein synthesis in the maturating messenger RNA. The reasons for these studies arises from evidence accrued thus far on the macromolecular syntheses which precede that of the secretory protein itself. Within these events, increased U1 snRNA accumulation occurs prior to the synthesis of mRNA. This accumulation, basis for the proposed project, is the result of an increase in the expression of the gene coding for U1 snRNA. Thus the study will be directed at the gene and its transcriptional activity. Isolation, cloning and characterization of the genes will lead to studies on transcription under cell-free conditions. Once the cell-free system is optimized, the testing of both cls and trans acting can be properly questioned, studies that will contribute towards the elucidation of the mechanism(s) underlying the event under analysis. The timeliness of this event is the fact that its production precedes that of its target, mRNA. U1 snRNA is a highly conserved molecule, thus information from our studies may be applicable to other forms of life, including humans. The relevance of the work lies in the conserved nature of U1 snRNA, the ubiquitousness of protein synthesis, and the role of U1 in the maturation of mRNA. Conservation is a high point of these molecules and its associated proteins (RNPs). Fungal snRNAs are bound specifically by Xenopus snRNP proteins. Epitopes recognized by several of the autoimmune antisera from lupus erythematosus patients have been conserved on snRNP proteins from humans to fungus.

该项目的长期目标是分析组织的合成- 具体产品使用合适的和富有成效的模型系统。 立即 该提案的目的是研究及时的组织特异性 刺激引起的U1小核RNA（U1 snRNA）的积累 转化为丝心蛋白产生腺体的蛋白质合成（模型系统）。 U1 snRNA在成熟信使的蛋白质合成中起重要作用 核糖核酸 这些研究的原因来自于迄今为止积累的证据 在分泌蛋白合成之前的大分子合成 本身 在这些事件中，U1 snRNA积累增加发生在 mRNA的合成。 这一积累，为拟议的基础 项目，是在基因编码的表达增加的结果， 对于U1 snRNA。 因此，这项研究将针对基因及其 转录活性 分离、克隆和鉴定 基因将导致在无细胞条件下的转录研究。 一旦无细胞系统得到优化， 表演可以适当地质疑，研究将有助于 阐明所分析事件的潜在机制。 的 这一事件的及时性是它的生产先于 它的靶基因mRNA U1 snRNA是一种高度保守的分子， 我们研究的信息可能适用于其他形式的生命， 包括人类 这项工作的相关性在于保守的性质， 蛋白质合成的普遍性，以及U1在 mRNA的成熟。 守恒是这些分子的一个最高点 及其相关蛋白（RNP）。 真菌snRNAs特异性结合 被非洲爪蟾snRNP蛋白质。 表位识别的几个 来自红斑狼疮患者的自身免疫性抗血清已经被保存， 从人类到真菌的snRNP蛋白。