REGULATION OF PROTEIN SYNTHESIS MODULATION BY TRNA

TRNA 对蛋白质合成的调控

• 批准号: 3936826
• 负责人: GRACIELA C CANDELAS
• 金额: --
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3936826
• 关键词: cell free system; eukaryote; gene expression; genetic regulation; genetic transcription; genetic translation; nucleic acid sequence; protein biosynthesis; transfer RNA

Synthesis of a secretory protein and the points at which the process may be regulated are being investigated using a pair of spider fibroin glands as a model system. The two outstanding characteristics of the system are that the glands can be cultured in vitro, and that through simple manipulations their synthetic activity may be virtually turned off, and then stimulated into a massive production of the protein product. Having characterized the gland, its product and also the template molecule, we have, thus far, been elucidated. All of them exert their regulation on a translational level, one at elongation, the other at the readout now to move on in our characterization, into posttranscriptional regulation. This is justified, because within an early transient wave of macromolecular syntheses preceding the template production, di novo synthesis of small nuclear RNAs and positive strong signals for a U1 RNA probe have been both detected in the stimulated glands. These results indicate that within the orchestration of fibroin production, the small RNAs play a vital role. Small RNAs are currently in the limelight of gene expression because of their proven role(s) in the postranscriptional processing of transcripts in eukaryotes. We, therefore, aim to characterize the small RNAs, particularly the U ones, because of their well established roles as transcript maturators. In view of our preliminary observations, we will explore the system for tissue specific expressions of these molecules, and establish kinetic correlations between selective U gene expressions and elicited fibroin synthesis.

分泌蛋白的合成及其合成点 过程可能受到监管正在使用一对进行调查 蜘蛛丝蛋白腺作为模型系统。 两位杰出的 该系统的特点是可以培养腺体 体外，并通过简单的操作合成 活动可能实际上被关闭，然后被刺激进入 蛋白质产品的大规模生产。 已表征 腺体、它的产物以及模板分子，我们有， 至此，已经阐明。 他们都发挥着各自的监管作用 平移水平，一个在伸长率处，另一个在读数处 现在继续我们的表征，进入转录后 规定。 这是有道理的，因为在早期瞬态中 模板生产之前的大分子合成浪潮， 小核RNA的从头合成和阳性强信号 对于 U1 RNA 探针，在受刺激的情况下均已检测到 腺体。 这些结果表明，在编排的范围内 丝心蛋白的产生中，小RNA发挥着至关重要的作用。 小RNA 目前在基因表达领域备受关注，因为它们 在转录后处理中已证实的作用 在真核生物中。 因此，我们的目标是表征小 RNA， 尤其是美国大学，因为他们的角色已经确立 作为转录成熟剂。 根据我们的初步观察， 我们将探索这些组织特异性表达的系统 分子，并建立选择性 U 之间的动力学相关性 基因表达并引发丝素合成。