REGULATION OF PROTEIN SYNTHESIS MODULATION BY TRNA

分泌型蛋白质的合成及其与蛋白质合成的关系 可能被监管的过程正在使用一对 以蜘蛛丝素腺体为模型系统。两大杰出人物 该系统的特点是腺体可以培养 在体外，通过简单的操作，他们的合成 活动可能被虚拟地关闭，然后被刺激成一种 蛋白质产品的大规模生产。有了特征 腺体，它的产物和模板分子，我们有， 到目前为止，已经被阐明。他们都把自己的规则用在 一种平移级别，一个在伸长时，另一个在读出时 现在继续我们的描述，进入转录后 监管。这是合理的，因为在早期的瞬变中 在模板生产之前的一波大分子合成， 小核RNA的双从头合成与正向强信号 对于一个U1 RNA探针都在受刺激的 腺体。这些结果表明，在 丝素蛋白的生产中，小RNA起着至关重要的作用。小RNA 目前处于基因表达的聚光灯下，因为他们的 已证实的角色(S)在文字记录的翻译后处理中的作用 在真核生物中。因此，我们的目标是描述小RNA的特征， 尤其是U型，因为他们已经确立了自己的角色 作为成绩单成熟者。根据我们的初步观察， 我们将探索这些基因的组织特异性表达系统 分子，并建立选择性使用之间的动力学关联 基因表达和诱导丝素蛋白合成。

Synthesis of a secretory protein and the points at which the process may be regulated are being investigated using a pair of spider fibroin glands as a model system. The two outstanding characteristics of the system are that the glands can be cultured in vitro, and that through simple manipulations their synthetic activity may be virtually turned off, and then stimulated into a massive production of the protein product. Having characterized the gland, its product and also the template molecule, we have, thus far, been elucidated. All of them exert their regulation on a translational level, one at elongation, the other at the readout now to move on in our characterization, into posttranscriptional regulation. This is justified, because within an early transient wave of macromolecular syntheses preceding the template production, di novo synthesis of small nuclear RNAs and positive strong signals for a U1 RNA probe have been both detected in the stimulated glands. These results indicate that within the orchestration of fibroin production, the small RNAs play a vital role. Small RNAs are currently in the limelight of gene expression because of their proven role(s) in the postranscriptional processing of transcripts in eukaryotes. We, therefore, aim to characterize the small RNAs, particularly the U ones, because of their well established roles as transcript maturators. In view of our preliminary observations, we will explore the system for tissue specific expressions of these molecules, and establish kinetic correlations between selective U gene expressions and elicited fibroin synthesis.