CALCIUM ACTIVATED PROTEASE IN CATARACT FORMATION

描述：该项目的总体目标是了解 钙激活蛋白在正常晶状体和晶状体中的作用 正在经历白内障形成的。当前的应用程序主要集中在 提供了晶状体中钙蛋白II激活的详细机制 通过三个目标：目标1将描述活动的序列 CaPin II在晶状体中的形式，并考虑可能的易位和 钙蛋白酶II在晶状体膜上的激活。目标2将描述 晶状体不溶率的特定成分对其性能的影响 钙蛋白酶II的激活。具体地说，晶状体膜的影响 将对组件进行评估，并将Calain II本地化到 将对不溶颗粒进行研究。一种钙蛋白酶的蛋白质激活剂 镜头中的II将被考虑。目标3将决定 测量表达对晶状体中钙蛋白酶II活性的mRNA表达 Calain II基因的表达。

DESCRIPTION: The overall goal of the project has been to understand the role of calcium activated proteolysis in normal lens and lenses undergoing cataractogenesis. The current application concentrates on providing a detailed mechanism for activation of calpain II in lens through three aims: Aim 1 will characterize the sequence of the active form of calpain II in lens and consider the possible translocation and activation of calpain II on lens membranes. Aim 2 will characterize the influence of specific components of the lens insoluble fraction on calpain II activation. Specifically the influence of lens membrane components will be evaluated and the localization of calpain II to the insoluble pellet will be studied. A proteinaceous activator for calpain II in lens will be considered. Aim 3 will determine the influence of mRNA expression on calpain II activity in lens by measuring expression of calpain II mRNA.