CALCIUM ACTIVATED PROTEASE IN CATARACT FORMATION

白内障形成中的钙激活蛋白酶

• 批准号: 2834514
• 负责人: THOMAS R SHEARER
• 金额: $ 5.58万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2834514
• 关键词: SDS polyacrylamide gel electrophoresis; calcium; calpain; cataract; enzyme activity; enzyme induction /repression; enzyme mechanism; enzyme model; gene expression; human tissue; laboratory rat; lens; mass spectrometry; messenger RNA; protein sequence; proteolysis; tissue /cell culture

DESCRIPTION: The overall goal of the project has been to understand the role of calcium activated proteolysis in normal lens and lenses undergoing cataractogenesis. The current application concentrates on providing a detailed mechanism for activation of calpain II in lens through three aims: Aim 1 will characterize the sequence of the active form of calpain II in lens and consider the possible translocation and activation of calpain II on lens membranes. Aim 2 will characterize the influence of specific components of the lens insoluble fraction on calpain II activation. Specifically the influence of lens membrane components will be evaluated and the localization of calpain II to the insoluble pellet will be studied. A proteinaceous activator for calpain II in lens will be considered. Aim 3 will determine the influence of mRNA expression on calpain II activity in lens by measuring expression of calpain II mRNA.

描述：该项目的总体目标是了解 钙激活蛋白水解在正常透镜和晶状体中的作用 正在经历白内障形成 本申请集中于 提供了激活透镜中钙蛋白酶II的详细机制 通过三个目标：目标1将描述活性物质的序列 透镜中钙蛋白酶II的形式，并考虑可能的易位， 激活透镜膜上的钙蛋白酶II。 目标2将描述 透镜不溶部分的特定组分对 钙蛋白酶II激活。 特别是透镜膜的影响 将对这些成分进行评价，并将钙蛋白酶II定位于 将研究不溶性颗粒。 钙蛋白酶的蛋白质类激活剂 将考虑使用透镜中的II。 目标3将确定 通过测量表达量测定透镜中钙蛋白酶II活性的mRNA表达 钙蛋白酶II mRNA的表达。