A high etendue Raman spectrometer for protein and biological measurement with flourescence subtraction

用于蛋白质和生物测量（荧光减法）的高集光率拉曼光谱仪

• 批准号: 131695
• 金额: $ 3.15万
• 依托单位: IS-INSTRUMENTS LIMITED
• 依托单位国家: 英国
• 项目类别: Feasibility Studies
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=131695
• 关键词: etendue; Raman; spectrometer; protein; biological

n this project ISI will explore new innovative solutions in protein characterisation which is crucial to engineering methods to develop proteins. Raman systems have been successfully used to study a variety of biological samples, including transmission raman measurement targeted at the detection of cancer. However these target proteins and biological samples are often inhomogeneous in nature and can suffer from a high fluorescent background. This study will investigate the feasibility of producing a new, dual wavelength, high etendue Raman spectrometer that can remove the fluorescent background whilst making both transmission and spatially offset bulk measurements of these samples. This system should allow faster characterisation with better bulk measurement, improving the accuracy of the observation.

在这个项目中，ISI将探索蛋白质表征的创新解决方案，这对开发蛋白质的工程方法至关重要。拉曼系统已经成功地用于研究各种生物样品，包括针对癌症检测的透射拉曼测量。然而，这些靶蛋白和生物样品在性质上往往是不均匀的，并且可能受到高荧光背景的影响。本研究将探讨生产一种新的双波长、高通量拉曼光谱仪的可行性，该光谱仪可以去除荧光背景，同时对这些样品进行透射和空间偏移量测。该系统应该允许更快的表征和更好的批量测量，提高观察的准确性。