A high etendue Raman spectrometer for protein and biological measurement with flourescence subtraction

在这个项目中，ISI将探索蛋白质表征的新的创新解决方案，这对开发蛋白质的工程方法至关重要。拉曼系统已成功地用于研究各种生物样品，包括以检测癌症为目标的透射拉曼测量。然而，这些靶蛋白和生物样品通常在性质上是不均匀的，并且可能遭受高荧光背景。本研究将探讨生产一种新的，双波长，高光学扩展量的拉曼光谱仪，可以消除荧光背景，同时使这些样品的传输和空间偏移批量测量的可行性。该系统应允许更快的表征，更好的批量测量，提高观察的准确性。

n this project ISI will explore new innovative solutions in protein characterisation which is crucial to engineering methods to develop proteins. Raman systems have been successfully used to study a variety of biological samples, including transmission raman measurement targeted at the detection of cancer. However these target proteins and biological samples are often inhomogeneous in nature and can suffer from a high fluorescent background. This study will investigate the feasibility of producing a new, dual wavelength, high etendue Raman spectrometer that can remove the fluorescent background whilst making both transmission and spatially offset bulk measurements of these samples. This system should allow faster characterisation with better bulk measurement, improving the accuracy of the observation.