CORNEAL EPITHELIAL CELL SURFACE GLYCOCONJUGATES

这项由两部分组成的研究将研究角膜感染的发病机制， 角膜上皮细胞迁移的机制。 (1)铜绿假单胞菌和S. Aureus到角膜： 本研究旨在进一步阐明细菌附着的机制 角膜上皮。 各种兔角膜的化学结构 上皮细胞表面鞘糖脂（GSL）结合P。 aeruginosa和S.金黄色葡萄球菌将被阐明。 我们将尝试确定， 用抗GSL单克隆抗体进行组织化学染色。 aeruginosa和S.在兔角膜上皮中发现了金黄色反应性GSL， 培养物（i）存在于正常人和兔角膜上皮上， 体内，（ii）掩蔽直至损伤导致暴露，或（iii）存在 仅在损伤的人和兔角膜上皮上。 实验也将 以确定是否可能（i）阻止遵守 各种抗GSL单克隆抗体对角膜损伤的影响 抗体、糖、寡糖、凝集素和糖苷酶，以及（ii） 不同糖类对角膜损伤后粘附细菌的抑制作用 部分。 希望这项研究有助于我们了解 细菌性角膜溃疡的发病机制。 (2)角膜上皮伤口愈合：本项目的目标是 进一步阐明角膜上皮细胞迁移的机制。 的 兔角膜移行特异性GSL的化学结构 将阐明上皮。 我们将尝试通过组织化学 技术是否迁移特异性GSL兔角膜。 它还将 确定是否有可能刺激角膜上皮 在器官培养中通过加入纯化的 向培养基中加入迁移特异性GSL。 实验也将 进行以确定蛋白酶是否在角膜上皮中发挥作用 通过暴露掩蔽的角膜上皮细胞表面GSL进行细胞迁移， 这些GSL通过与细胞膜相互作用来影响细胞迁移。 细胞外基质的大分子。 希望本研究 将有助于我们了解这些因素，调节角膜 上皮伤口愈合。

This two-part study will examine the pathogenesis of corneal infection and mechanisms of corneal epithelial cell migration. (1) Adherence of P. aeruginosa and S. Aureus to the Cornea: The goals of this study are to further elucidate the mechanism of bacterial attachment to corneal epithelium. The chemical structure of various rabbit corneal epithelial cell surface glycosphingolipids (GSL) which bind to P. aeruginosa and S. aureus will be elucidated. We will try to determine by histochemical techniques using anti-GSL monoclonal antibodies whether P. aeruginosa nd S. aureus-reactive GSL found in rabbit corneal epithelium in culture are (i) present on normal human and rabbit corneal epithelium in vivo, (ii) masked until injury causes them to be exposed, or (iii) present only on injured human and rabbit corneal epithelium. Experiments will also be performed to determine whether it is possible (i) to block the adherence of bacteria to the injured cornea by various anti-GSL monoclonal antibodies, sugars, oligosaccharides, lectins, and glycosidases and (ii) to elute the adherent bacteria from the injured corneas by various saccharide moieties. It is hoped that this study will contribute to our understanding of the pathogenesis of bacterial corneal ulcers. (2) Corneal Epithelial Wound Healing: The goal of this project are to clarify further the mechanism of corneal epithelial cell migration. The chemical structure of the migration-specific GSL of rabbit corneal epithelium will be elucidated. We will try to establish by histochemical techniques whether migration-specific GSL of rabbit corneas. It will also be determined whether it is possible to stimulate corneal epithelial migration of wounded corneas in organ culture by the addition of purified migration-specific GSL to the culture media. Experiments will also be performed to establish whether proteases play a role in corneal epithelial cell migration by exposing the masked corneal epitheal cell surface GSL and that these GSL influence cell migration by interacting with the macromolecules of the extracellular matrix. It is hoped that this study will contribute to our understanding of those factors that modulate corneal epithelial wound healing.