FT MASS SPECTROMETRY FOR BIOMOLECULE ANALYSIS

用于生物分子分析的 FT 质谱法

• 批准号: 2176254
• 负责人: ALAN G MARSHALL
• 金额: $ 27.29万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-11-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2176254
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; carbohydrate structure; chemical structure; computer simulation; interferometry; ion cyclotron resonance spectrometry; ionization; ions; lasers; macromolecule; magnetic field; mass spectrometry; oligosaccharides; protein structure; structural biology; superconductivity

DESCRIPTION: (Adapted from the applicant's abstract) It is proposed to design, construct, test, and systematically refine a state-of-the-art Fourier transform ion cyclotron resonance mass spectrometer built around a 9.4 tesla superconducting magnet. The instrument will optimized for unsurpassed sensitivity, mass resolution, and mass accuracy for singly- and multiply- charged macromolecular ions up to 150,000 dalton. The instrument will incorporate the following features: electrospray, Cs+ liquid secondary ion, and matrix-assisted laser desorption ion sources; a novel ion transfer and trapping method; stored wave form dual-channel broadband dipolar and quadrupolar excitation; fast data acquisition and processing for wide mass range at high mass resolving power; and rf- linearized ion trap for increased upper mass limit, more accurate mass measurement, and more accurate ion relative abundances, higher- resolution MS/MS performance, increased dynamic range, and linearized detection. Theoretical numerical simulations will be used to determine the mechanism(s) for ion trapping and mass spectral line- broadening for high-mass ions (particularly singly-charged ions), including applied static and rf electric field, fully mapped magnetic field, and coulomb forces between ions. The results of those tests will guide the design of ion injection, ion trapping, and excitation/detection techniques designed to optimize high-mass ion analysis. Applications for these technical developments include identification, sequencing, and structural analysis of phospholipids, oligosaccharides, proteins, oligonucleotides, and complex unseparated mixtures.

描述：(改编自申请人的摘要)建议 设计、构建、测试并系统地提炼最先进的 傅里叶变换离子回旋共振质谱仪 9.4特斯拉超导磁体。仪器将针对以下方面进行优化 无与伦比的灵敏度、质量分辨率和质量精度 以及高达15万道尔顿的多电荷大分子离子。这个 仪器将包括以下功能：电喷雾、Cs+ 液体二次离子和基质辅助激光解吸离子源； 一种新的离子转移和捕获方法；存储波形双通道 宽带偶极和四极激励；快速数据采集和 在高质量分辨率下进行宽质量范围的处理； 线性化离子陷阱，提高了质量上限，质量更准确 测量，以及更准确的离子相对丰度，更高- 分辨率MS/MS性能，提高动态范围，线性化 侦测。理论数值模拟将被用来确定 离子俘获和谱线展宽的机理(S) 高质量离子(特别是单电荷离子)，包括外加 静电场和射频电场、全映射磁场和库仑 离子之间的作用力。这些测试的结果将指导设计 离子注入、离子捕获和激发/检测技术 旨在优化高质量离子分析。这些项目的申请 技术发展包括识别、测序和结构 分析磷脂、低聚糖、蛋白质、寡核苷酸、 和复杂的未分离混合物。