FT MASS SPECTROMETRY FOR BIOMOLECULE ANALYSIS

我们建议升级我们的尼科莱FTMS-1000傅里叶变换质量 将光谱仪的样品转移至具有8特斯拉磁体的尼科莱FTMS-2000。 本 真空系统和推车，电池组件和安装，以及真空泵将 换新版本，励磁放大器升级 更高的输出电压。 现有的控制台和数据系统将 保留。 新系统将提供定性和定量的信息 改进. 最重要的新特性将是 高质量分辨率的可靠气相色谱/FT/MS; 实验不能在FTMS-1000上进行，除非特别 选择样本 此外，几项量化改进也是如此。 大到提供质量上的新能力：（a）快速更换样品 (10与FTMS-1000上目前要求的最多2小时相比，最少2分钟）;（B） 改善的信噪比（至少5倍），由于离子 高压下形成，低压下检测;（c）增强 由于检测器处的压力较低，分辨率（至少为5倍）; (d)两英寸的样品池，而不是一英寸，导致加倍， 信噪比和质量分辨率加倍，以及改进的 由于库仑展宽减少而形成的线形状;（e）网格而不是实心 细胞板，在GC期间提供更有效的抽气电导 和/或改变样品;（f）降低检测器处的压力， 用于激光解吸/电离过程中的精确质量测定 实验 磁场强度越大，信噪比越高 （2.66X）和质量分辨率（2.66X），并减少数据采集时间（7.1X）。 分时“定制”激励（即，“随机”激励）将是 为FT/MS开发，提供软件可互换性：（a）平面 用于精确同位素比测量的激发功率，（B）加窗 用于增加动态范围或具有高质量分辨率的MS/MS的激发， 以及（c）第一同时多离子监测能力。 这些 方法将应用于合成和合成的质谱分析， 天然药物和代谢物。

We propose to upgrade our Nicolet FTMS-1000 Fourier transform mass spectrometer to a Nicolet FTMS-2000 with 8 tesla magnet. The present vacuum system and cart, cell assembly and mount, and vacuum pumps will be exchanged for new versions, and the excitation amplifier will be upgraded to higher output voltage. The existing console and data system will be retained. The new system will provide both qualitative and quantitative improvements. The most important new feature will be the capability for reliable gas chromatography/FT/MS at high mass resolution; those experiments cannot be performed on the FTMS-1000, except for specially chosen samples. In addition, several quantitative improvements are so great as to offer qualitatively new capability: (a) rapid sample changing (10 min compared to up to 2 hours presently required on the FTMS-1000); (b) improved signal-to-noise ratio (at least a factor of 5), due to ion formation at high pressure with detection at low pressure; (c) enhanced resolution (at least a factor of 5) due to lower pressure at the detector; (d) two-inch sample cell rather than one-inch, resulting in a doubling of signal-to-noise ratio and doubling of mass resolution, as well as improved line shape due to reduced Coulomb broadening; (e) mesh rather than solid cell plates, providing for more efficient pump-down conductance during GC and/or on changing samples; (f) reduced pressure at the detector, allowing for exact mass determination during laser desorption/ionization experiments. The higher magnetic field will increase signal-to-noise ratio (2.66X) and mass resolution (2.66X) and reduce data acquisition time (7.1X). Time-shared "tailored" excitation (i.e., "stochastic" excitation) will be developed for FT/MS, providing software-interchangeable: (a) flat excitation power for precise isotope-ratio measurement, (b) windowed excitation for increased dynamic range or MS/MS with high mass resolution, and (c) the first simultaneous multiple-ion monitoring capability. These methods will be applied toward mass spectrometric analysis of synthetic and natural drugs and metabolites.