MAPPING SURFACE CONTACTS IN BIOMACROMOLECULE COMPLEXES

绘制生物大分子复合物中的表面接触图

• 批准号: 6179623
• 负责人: ALAN G MARSHALL
• 金额: $ 19万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-11-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6179623
• 关键词: carbon; cyclin dependent kinase; deuterium; epitope mapping; infrared spectrometry; interferometry; ion cyclotron resonance spectrometry; macromolecule; protein structure; stable isotope; structural biology; surface property; troponin; tumor suppressor proteins

DESCRIPTION: (Adapted from the applicant's abstract) This project aims to identify and map the surface contacts between two or non-covalently bound proteins by combining three general techniques which the P.I. states that he first devised: (a) isotopic amplifications by expression and purification of proteins doubly-depleted in C-13 and N-15; (b) hydrogen/deuterium exchange of individual proteins and protein complexes in solution followed by rapid quenching, enzymatic cleavage to produce dozens of peptide fragments, and prompt ultra high-resolution mass analysts to determine extend of deuterium incorporation (and thus solvent accessibility to) the backbone of amide protons for each fragment; and (c) various Fourier transform ion cyclotron resonance (FT-ICR) techniques (micro/LC/ESI, on-line desalting, external ion cyclotron accumulation, SWIFT ion selection, digital heterodyne/digital quadrature detection, charge state deconvolution and isotopic deconvolution) needed for unequivocal interpretation of the results. The INK4 family of tumor suppressor proteins (p15,p16,p18,p19) acts in part by forming non-covalent complexes with cyclin-dependent kinases (cdk, 34-37 kDa), and cyclin (34 kDa). Only the smallest of these is accessible by high-resolution NMR. The applicants shall begin by mapping the surface contacts in binary complexes of INK4 protein, cdk, and cyclin and various recombinant variants, and then extend the same approach to epitope mapping of antigen: antibody complexes, to the troponin family of muscle proteins.

描述：（改编自申请人摘要）本项目旨在

项目成果

Dispersion versus absorption (DISPA) method for automatic phasing of Fourier transform ion cyclotron resonance mass spectra.

用于傅里叶变换离子回旋共振质谱自动定相的色散与吸收 (DISPA) 方法。

• DOI: 10.1002/rcm.1290010209
• 发表时间: 1987
• 期刊: Rapid communications in mass spectrometry : RCM
• 作者: Craig,EC;Santos,I;Marshall,AG
• 通讯作者: Marshall,AG

Attomole biomolecule mass analysis by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance.

通过基质辅助激光解吸/电离傅立叶变换离子回旋共振进行阿托莫尔生物分子质量分析。

• DOI: 10.1021/ac00118a017
• 发表时间: 1995
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Solouki,T;Marto,JA;White,FM;Guan,S;Marshall,AG
• 通讯作者: Marshall,AG

Pulse timing and optical interface between a neodymium: yttrium aluminum garnet laser and a Fourier transform ion cyclotron resonance mass spectrometer.

钕：钇铝石榴石激光器和傅里叶变换离子回旋共振质谱仪之间的脉冲定时和光学接口。

• DOI: 10.1002/rcm.1290050309
• 发表时间: 1991
• 期刊: Rapid communications in mass spectrometry : RCM
• 作者: Liang,Z;Ricca,TL;Marshall,AG
• 通讯作者: Marshall,AG

Gas phase RNA and DNA ions 2. Conformational dependence of the gas-phase H/D exchange of nucleotide-5'-monophosphates.

气相 RNA 和 DNA 离子 2. 5-单磷酸核苷酸气相 H/D 交换的构象依赖性。

• DOI: 10.1016/s1044-0305(01)00260-4
• 发表时间: 2001
• 期刊: Journal of the American Society for Mass Spectrometry
• 影响因子: 3.2
• 作者: Freitas,MA;Marshall,AG
• 通讯作者: Marshall,AG

Laser desorption Fourier transform ion cyclotron resonance mass spectrometry vs. fast atom bombardment magnetic sector mass spectrometry for drug analysis.

用于药物分析的激光解吸傅里叶变换离子回旋共振质谱与快原子轰击扇形磁质谱。

• DOI: 10.1021/ac00291a043
• 发表时间: 1985
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Shomo2nd,RE;Marshall,AG;Weisenberger,CR
• 通讯作者: Weisenberger,CR