DEVELOPMENTAL REGULATION IN DROSOPHILA

我们最近发现，一个显着的内部重塑， 果蝇卵巢的结构发生在幼虫的末端 期 这是当卵巢划分成卵巢管是 由内部规则间隔的末端细丝的形成引发， 卵巢 3龄幼虫卵巢图 图中显示了成年卵巢管前端的丝形成 下面 我们的主要目标是研究卵巢的发育， 幼虫阶段，并了解如何终端丝出现。 这 工作将分为三个部分： 1. 野生型幼虫卵巢形态发生的详细分析 使用光学和电子显微镜，增强子陷阱标记和镶嵌 分析. 2. 分子和遗传特性的bric-a-brac和 fs（2）eoP.L3，末端正确形成所需的两个基因 细丝。 3. 筛选其他参与发育的基因， 幼虫子房 除了形成终端丝极叠层所需要的之外， 在腿和天线的开发过程中也需要砖块。 我们有 发现了一种简单的突变果蝇， 远端跗骨节到第1节的身份，这 转化取决于bric-a-brac基因的剂量。 在 此外，砖-a-brac是表示在一个不连续的梯度沿着 发育腿的近端-远端轴。 作为第二部分， 格兰特，我们建议确定如何表达的小块梯度 并确定在腿部发育过程中与小摆设相互作用的基因 发展

We have recently found that a remarkable remodeling of the internal structure of the Drosophila ovary takes place at the end of the larval period. This is when the partitioning of the ovary into ovarioles is initiated by the formation of regularly spaced terminal filaments inside the ovary. Drawings of the 3rd instar larval ovary after terminal filament formation and of the anterior tip of an adult ovariole are shown below. Our major goal is to study the development of the ovary during larval stages and to understand how the terminal filaments arise. This work will be divided into three parts: 1. Detailed analysis of the morphogenesis of wild type larval ovaries using light and electron microscopy, enhancer trap markers and mosaic analysis. 2. Molecular and genetic characterization of bric-a-brac and fs(2)eoP.L3, two genes required for the proper formation of terminal filaments. 3. A screen for other genes that are involved in the development of the larval ovary. In addition to being required for terminal filament stack formation, bric-a-brac is also required during leg and antenna development. We have discovered that a bric-a-brac mutant fly has a homeotic transformation of distal tarsal segments to a segment 1 identity, and that this transformation is dependent on the dosage of the bric-a-brac gene. In addition, bric-a-brac is expressed in a discontinuous gradient along the proximal-distal axis of the developing leg. As the second part of this grant, we propose to determine how the bric-a-brac gradient of expression is formed and to identify genes that interact with bric-a-brac during leg development.