DEVELOPMENTAL REGULATION IN DROSPHILLA

基因bric a brac（bab）是一种转录调节因子， 卵巢，腿和触角的正常发育所需的， 果蝇 我们的长期目标是 了解bab的功能，并使用bab作为进入 四肢和卵巢发育情况调查。我们建议继续我们的 通过以下具体目标开展研究： 1. bab的功能分析。 我们有广泛的知识， bab的基因结构、遗传学和表达模式。 我们有 确定了bab的靶基因（它结合自己的基因座），并表明， BAB可以抑制其自身的生产。 我们目前的目标是了解 bab在分子水平上是如何运作的 为此，我们将调查 babII蛋白与启动子结合的结果。 另外我们 将对babII蛋白进行结构/功能分析， BTB结构域和psq基序的功能。 2.在腿部发育过程中与bab相互作用的基因的筛选。Bab 提供了一个独特的工具，在了解过程中的模式形成 在果蝇的腿上，特别感兴趣的是 近端-远端轴。 bab被要求给三个中央 跗骨的片段他们的身份，因此，基因必须是 参与解释沿轴沿着的位置线索的过程。 了解bab如何与其他基因相互作用以解释位置 这些信息将使我们深入了解近端- 远轴形成。 使用遗传学方法，我们将识别基因 与宝宝互动。 这些基因将被研究，以确定其 腿发育的作用。 3. NTF（无末端）的遗传和分子特征 丝）基因。 我们最近发现了ntf基因， 确定的基因（除bab外）是终末 丝形成 我们的长期目标是了解 发育过程在卵巢发育期间起作用。 我们正在采取 一个遗传学的方法来实现这一目标，确定基因所需的， 卵巢发育，然后分析这些基因。 分析bab 告诉了我们很多关于卵巢发育的信息， ntf基因的表征应该同样提供信息。

The gene bric a brac (bab) is a transcriptional regulator that is required for the proper development of the ovary, leg and antenna in Drosophila melanogaster. Our long term goal is to have a molecular understanding of how bab functions, and to use bab as an entryway to the investigation of limb and ovary development. We propose to continue our studies by means of the following specific aims: 1. A functional analysis of bab. We have extensive knowledge of the gene structure, genetics and expression pattern of bab. We have identified a target gene for bab (it binds its own locus) and shown that bab can repress its own production. Our current goal is to understand how bab functions at a molecular level. To this we will investigate the consequence of babII protein binding to a promoter. In addition, we will do structure/function analysis of the babII protein, investigating the function of the BTB domain and psq motif. 2. A screen for genes that interact with bab during leg development. bab provides a unique tool in understanding the process of pattern formation in the leg of Drosophila, with specific interest in the formation of the proximal-distal axis. bab is required to give the three central segments of the tarsus their identity, therefore the gene must be involved in the process of interpreting positional cues along the axis. Understanding how bab interacts with other genes to interpret positional information will give insight into the molecular mechanism of proximal- distal axis formation. Using a genetic approach, we will identify genes that interact with bab. These genes will be studied to determine their role in leg development. 3. A genetic and molecular characterization of the ntf (no terminal filaments) gene. We have recently identified that ntf gene, the second gene identified (in addition to bab) that is required for terminal filament formation. Our long term goal is to understand how developmental processes work during ovary development. We are taking a genetic approach to this goal, identifying genes that are required for ovary development and then analyzing these genes. Analysis of bab has told us a great deal about ovary development, the molecular and genetic characterization of the ntf gene should be just as informative.