NIM--A GENE AND MITOTIC REGULATION
NIM--基因和有丝分裂调节
基本信息
- 批准号:2181486
- 负责人:
- 金额:$ 23.24万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1989
- 资助国家:美国
- 起止时间:1989-07-01 至 1996-06-30
- 项目状态:已结题
- 来源:
- 关键词:Aspergillus Xenopus oocyte alleles cell cycle cyclins enzyme activity gene expression genetic promoter element genetic regulation high performance liquid chromatography immunoprecipitation microinjections molecular cloning nucleic acid sequence polymerase chain reaction posttranslational modifications protein kinase tissue /cell culture western blottings
项目摘要
Great progress has recently been made towards an understanding of the
regulation of the eukaryotic cell cycle at the molecular level. A
consensus has evolved indicating that activation of a single protein
kinase, p34(cdc2), is Sufficient to initiation mitosis or meiosis in all
eukaryotes. However, in Aspergillus nidulans it is necessary to activate
both the p34(cdc2) protein kinase and the NIMA protein kinase (product of
nimA gene) in order to initiate mitosis (Osmani et al., 1991a; 1991 b).
To fully understand the regulation of mitosis it is therefore imperative
to understand the role of the NIMA protein kinase. The work proposed is
aimed at further defining the role of nimA in the regulation of mitosis.
The work will provide knowledge, clones, and antibodies that will be of
use in understanding the uncontrolled cell cycle associated with cancer.
Regulation of NIMA expression in G2 is largely under post-transcriptional
control. Deletion analysis has defined a potential translational
regulatory sequence in nima mRNA 5' to the NIMA open reading frame. This
region is to be analyzed in detail and the mechanism of its regulation
determined. There appears to be a requirement for cyclin B during G2 for
the increased expression of NIMA activity. The level at which cyclin B
is required for nimA activity will be determined utilizing mutation of
cyclin B. The stability of NIMA will be analyzed because as cells
progress through mitosis NIMA activity drops and we will relate any
degradation of NIMA protein observed at mitosis to the degradation
pathway that destroys cyclin protein during mitosis. Several genes have
been isolated from species ranging from yeast to mice that encode
putative protein kinases with most similarity to nimA. These will be
tested for functional complementation of nimA5 in A. nidulans. We have
isolated a functional nimA homolog from Neurospora crassa. Using the
available nimA like sequences PCR primers are to be designed and used to
clone functional homologs of nimA from Xenopus. Antibodies, and
oligonucleotide direct ablation of nimA in Xenopus oocytes and extracts
derived from them will be used to relate nimA to maturation promotion
factor (MPF) and also to two other newly isolated non-p34(cdc2)
containing MPF activities. Finally, genetic screens are to be employed
to identify, and then isolate, other gene functions of Aspergillus
nidulans that are involved in the nimA cell cycle regulatory system.
最近,在了解人类的历史方面取得了很大进展
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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STEPHEN A OSMANI其他文献
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{{ truncateString('STEPHEN A OSMANI', 18)}}的其他基金
The role of the NIMA kinase in mitotic regulation
NIMA 激酶在有丝分裂调节中的作用
- 批准号:
7911004 - 财政年份:2009
- 资助金额:
$ 23.24万 - 项目类别:
THE NIMA PROTEIN KINASE IN MITOTIC REGULATION
有丝分裂调节中的 NIMA 蛋白激酶
- 批准号:
6519344 - 财政年份:1989
- 资助金额:
$ 23.24万 - 项目类别:
ROLE OF THE NIM-A GENE IN MITOTIC REGULATION
NIM-A 基因在有丝分裂调节中的作用
- 批准号:
3301218 - 财政年份:1989
- 资助金额:
$ 23.24万 - 项目类别:
The role of the NIMA kinase in mitotic regulation
NIMA 激酶在有丝分裂调节中的作用
- 批准号:
7490574 - 财政年份:1989
- 资助金额:
$ 23.24万 - 项目类别:
THE NIMA PROTEIN KINASE IN MITOTIC REGULATION
有丝分裂调节中的 NIMA 蛋白激酶
- 批准号:
6320385 - 财政年份:1989
- 资助金额:
$ 23.24万 - 项目类别:
ROLE OF THE NIM-A GENE IN MITOTIC REGULATION
NIM-A 基因在有丝分裂调节中的作用
- 批准号:
3301221 - 财政年份:1989
- 资助金额:
$ 23.24万 - 项目类别:
The role of the NIMA kinase in mitotic regulation
NIMA 激酶在有丝分裂调节中的作用
- 批准号:
7123471 - 财政年份:1989
- 资助金额:
$ 23.24万 - 项目类别:
The role of the NIMA kinase in mitotic regulation
NIMA 激酶在有丝分裂调节中的作用
- 批准号:
8206580 - 财政年份:1989
- 资助金额:
$ 23.24万 - 项目类别:
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