BIOLOGICAL VARIATION AMONG AFRICAN TRYPANOSOMES

非洲锥虫的生物学变异

• 批准号: 2076258
• 负责人: John M. Mansfield
• 金额: $ 15.75万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-15 至 1998-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2076258
• 关键词: Cercopithecidae; Trypanosoma brucei rhodesiense; biomarker; complementary DNA; gene expression; gene targeting; laboratory mouse; molecular cloning; northern blottings; nuclear runoff assay; nucleic acid sequence; pathologic process; phenotype; polymerase chain reaction; pulsed field gel electrophoresis; restriction fragment length polymorphism; southern blotting; tissue /cell culture; transfection; virulence

African trypanosomes are the causative agents of human sleeping sickness as well as fatal disease in other animals. Biological variation among genetically related African trypanosomes is the central theme of this proposal, with the primary focus on virulence. The hypothesis addressed is that African trypanosomes regulate the clonal expression of virulence during infection of a mammalian host by differential expression of specific genes. The experimental foundation for the proposed studies includes a well characterized and clinically relevant Trypanosoma brucei rhodesiense model system of virulence, and a panel of differentially expressed genes cloned from high and low virulence trypanosomes. In this proposal, candidate virulence-associated genes will be selected and tested for functional and genetic linkages to specific virulence phenotypes. The results will provide new and relevant information on trypanosome cell biology and mechanisms of pathogenesis that may lead to new avenues for treatment or control of trypanosomiasis.

非洲锥虫是人类昏睡病的病原体 以及其他动物的致命疾病。生物多样性 基因相关的非洲锥虫是这一主题的中心 建议，主要集中在毒力。假设解决了 非洲锥虫调节毒力克隆表达 在感染哺乳动物宿主期间， 特定基因 拟议研究的实验基础 包括一种充分表征的和临床相关的布氏锥虫， 罗得西亚菌模型系统的毒力，和一组差异 从高毒力和低毒力锥虫克隆表达基因。在这 建议，候选的毒力相关基因将被选择， 测试了与特定毒力的功能和遗传联系 表型 结果将提供新的相关信息， 锥虫细胞生物学和可能导致 治疗或控制锥虫病的新途径。